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KAKUTANI Koji

    Pharmaceutical Research and Technology Institute　Professor
Last Updated :2024/04/25

Researcher Information

URL

J-Global ID

Research Interests

  • 静電気工学   Biological and Environmental Engineers   Plant Cell Biology   pharmacognosy   

Research Areas

  • Life sciences / Environmental and pharmaceutical development resources / Pharmacognosy

Published Papers

  • Yoshinori Matsuda; Koji Kakutani; Hideyoshi Toyoda
    American Journal of Civil Engineering and Architecture Science and Education Publishing Co., Ltd. 12 (1) 1 - 7 2328-398X 2024/01
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  • Koji Kakutani; Yoshinori Matsuda; Yoshihiro Takikawa; Hideyoshi Toyoda
    American Journal of Public Health Research Science and Education Publishing Co., Ltd. 11 (6) 211 - 218 2327-669X 2023/12
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  • Yoshinori Matsuda; Koji Kakutani; Hideyoshi Toyoda
    Agronomy MDPI AG 13 (7) 1954 - 1954 2023/07 
    This study developed an unattended electric weeder (UEW) to control floor weeds in an orchard greenhouse. The UEW was a motor-driven dolly equipped with a spark exposer. The spark exposer was constructed by applying an alternating voltage (10 kV) to a conductor net (expanded metal net). The charged conductor net (C-CN) discharged into the surrounding space. Wild oat and white clover were used as test weed species. Weed seedlings growing on the floor were grounded by the biological conductor and were subjected to a spark from the C-CN when they reached the discharge space. The spark-exposed seedlings were singed and shrunk instantaneously. In the present experiment, the UEW was remotely controlled to move on the soil-cover metal nets, which were laid on the floor to make a flat surface, in a stop-and-go manner, and to eject a spark to the weed seedlings that emerged from the floor. All of the mono- and dicotyledonous weed seedlings, which had been artificially sown on the floor, were completely eradicated using this method. Thus, this study provides an experimental basis for developing an unattended technique for controlling floor weeds in an orchard greenhouse.
  • Yutaka Kimura; Márk Z. Németh; Kana Numano; Asami Mitao; Tomomi Shirakawa; Diána Seress; Yoshihiro Takikawa; Koji Kakutani; Yoshinori Matsuda; Levente Kiss; Teruo Nonomura
    Agronomy MDPI AG 13 (5) 1204 - 1204 2023/04 
    In this study, we evaluated the effectiveness of hyperparasitic fungi in controlling powdery mildew (PM). In a greenhouse, we spray-inoculated single colonies of the melon PM-causing fungus Podosphaera xanthii strain KMP-6N at three different fungal developmental stages (i.e., 5, 10, and 15 days old) with spores of the hyperparasitic fungus Ampelomyces sp. strain Xs-q. After spray inoculation, we collected and counted KMP-6N conidia produced as asexual progeny from PM colonies using an electrostatic rotational spore collector. Collector insulator films were replaced at 24 h intervals until KMP-6N ceased to release additional progeny conidia. Conidial releases from each of the single Xs-q-inoculated KMP-6N colonies gradually reduced, then stopped within ca. 4 and 8 days of the first treatment in 5- and 10-day-old KMP-6N colonies, and within ca. 20 days of the second spray treatment in 15-day-old KMP-6N colonies, respectively. The total numbers of asexual progeny conidia collected from single 5-, 10-, and 15-day-old colonies were ca. 156, 1167, and 44,866, respectively. After electrostatic spore collection, conidiophores in Xs-q-uninoculated KMP-6N colonies appeared normal, whereas almost all conidiophores in 5- and 10-day-old Xs-q-inoculated KMP-6N colonies were completely deformed or collapsed due to the infection of the hyperparasitic fungus. This is the first study to apply electrostatic and digital microscopic techniques to clarify the impact of fungal hyperparasitism on mycohost survival, and, in particular, to assess quantitatively and visually the suppression of conidial release from any PM colonies infected with Ampelomyces.
  • Koji Kakutani; Yoshinori Matsuda; Hideyoshi Toyoda
    Agronomy MDPI AG 13 (2) 310 - 310 2023/01 
    A simple electrostatic apparatus that generates an arc discharge was devised to control adult houseflies emerging from a soil bed in a greenhouse. Adult houseflies emerging from a soil bed in a greenhouse are a potential vector of pathogenic Escherichia coli O157, carried by animal manure used for soil fertilization. A simple electrostatic apparatus that generates an arc discharge was devised to control these houseflies. The apparatus consisted of two identical metal nets; one was linked to a negative-voltage generator to create a negatively charged metal net (NC-MN), and the other was linked to a grounded line to create a grounded metal net (G-MN). A square insulator frame was placed between the two nets, separating them by 6 mm, and a plastic grating with multiple cells was placed beneath the G-MN to provide a climbing path (54 mm in height) to the arcing sites of the apparatus for adult houseflies emerging on the soil surface. Houseflies that climbed up the wall of the grating and reached the arcing zone were subjected to arc-discharge exposure from the NC-MN and thrown down onto the soil by the impact of the arcing. The impact was destructive enough to kill the houseflies. The structure of this apparatus is very safe and simple, enabling ordinary greenhouse workers to fabricate or improve it according to their own requirements. This study developed a simple and safe tool that provides a physical method to manage houseflies.
  • Shuka Ayabe; Yutaka Kimura; Naoki Umei; Yoshihiro Takikawa; Koji Kakutani; Yoshinori Matsuda; Teruo Nonomura
    Plants MDPI AG 11 (24) 3453 - 3453 2022/12 
    Powdery mildew fungi produce progeny conidia on conidiophores, and promote the spread of powdery mildew diseases by dispersal of the conidia from conidiophores in the natural environment. To gain insights and devise strategies for preventing the spread of powdery mildew infection, it is important to clarify the ecological mechanism of conidial dispersal from conidiophores. In this study, all of the progeny conidia released from single colonies of strawberry powdery mildew fungus (Podosphaera aphanis (Wallroth) U. Braun and S. Takamatsu var. aphanis KSP-7N) on true leaves of living strawberry plants (Fragaria × ananassa Duchesne ex Rozier cv. Sagahonoka) were consecutively collected over the lifetime of the colony with an electrostatic rotational spore collector (insulator drum) under greenhouse conditions, and counted under a high-fidelity digital microscope. The insulator drum consisted of a round plastic container, copper film, thin and transparent collector film, electrostatic voltage generator, and timer mechanism. When negative charge was supplied from the voltage generator to the copper film, the collector film created an attractive force to trap conidia. The electrostatically activated collector film successfully attracted progeny conidia released from the colony. Experiment was carried out at just one colony on one leaf for each month (in February, May, July, October, November, and December in 2021), respectively. Each collector film was exchanged for a new collector film at 24 h intervals until KSP-7N ceased to release progeny conidia from single colonies. Collection experiments were carried out to estimate the total number of conidia released from a single KSP-7N colony over a 35–45-day period after inoculation. During the fungal lifetime, KSP-7N released an average of 6.7 × 104 conidia from each of the single colonies at approximately 816 h. In addition, conidial release from KSP-7N colonies was largely affected by the light intensity and day length throughout a year; the number of conidia released from single KSP-7N colonies in night-time was clearly smaller than that in daytime, and the time of conidial release from single KSP-7N colonies was shorter by approximately 2 to 4 h in autumn and winter than in spring and summer. The ecological characteristics related to conidial releases from KSP-7N colonies will be helpful information for us to successfully suppress the spread of strawberry powdery mildews onto host plants under greenhouse conditions.
  • Yoshihiro Takikawa; Yoshinori Matsuda; Koji Kakutani; Teruo Nonomura; Hideyoshi Toyoda
    Horticulturae MDPI AG 8 (9) 764 - 764 2022/08 
    An unattended pest control system was developed to eliminate whiteflies (Bemisia tabaci) that settled on greenhouse tomato plants. The system exploited the whitefly’s habit of flying up from a plant that was mechanically tapped and then heading toward yellow objects. Remote-controlled dollies with arms that tapped plants and yellow-colored double-charged dipolar electric field screens (YDD-EFSs) (oppositely electrified transparent insulator tubes filled with yellow-colored water) attracted and trapped the whiteflies. The whiteflies flew up when the plants were mechanically tapped with the dolly’s arms during reciprocating movements and were subsequently trapped by YDD-EFSs that were automatically translocated to the target plants. The system was applied to rows of whitefly-infested tomato plants. Almost all whiteflies transferred to plants were successfully recovered by two dollies moving on either side of the plants, approaching all plants individually (via programmed movement). In summary, we present an efficient unattended method for controlling whiteflies on tomato plants in greenhouses.
  • Koji Kakutani; Yoshinori Matsuda; Teruo Nonomura; Hideyoshi Toyoda
    Horticulturae MDPI AG 8 (6) 543 - 543 2022/06 
    Electrostatic devices generating an electric field (EF) are promising tools for greenhouse tomato cultivation. In these devices, an EF is generated in the space surrounding an insulated conductor (IC) that is charged by a voltage generator. Thus, a physical force is exerted on any insect that enters the EF, as a negatively charged IC (NC-IC) pushes a negative charge (free electrons) out of the insect body. The insect is polarized positively to be attracted to the NC-IC, and a grounded metal net (G-MN) repels the insect. This dual function of the apparatus (insect capture and repulsion) is the core of the electrostatic pest-exclusion strategy. In this study, we applied various innovative EF-based devices to evaluate their efficacy in greenhouse tomato cultivation. Our objective was to determine the optimal apparatus for simple, inexpensive construction by greenhouse workers. The results of this study will contribute to the development of sustainable pest-management protocols in greenhouse horticulture.
  • Shota Iwasaki; Naoko Okada; Yutaka Kimura; Yoshihiro Takikawa; Tomoko Suzuki; Koji Kakutani; Yoshinori Matsuda; Yuling Bai; Teruo Nonomura
    Agriculture MDPI AG 12 (2) 254 - 254 2022/02 
    Type I trichomes of tomato leaves (Solanum lycopersicum Mill. cv. Moneymaker), as outgrowths of the plant epidermis, are suitable for monitoring infection processes of powdery mildew species using a high-fidelity digital microscope (DM) without fungal staining. On the trichomes, tomato powdery mildew (Pseudoidium neolycopersici L. Kiss) isolate KTP-03 produced a maximum of four vigorously elongated hyphae per conidium, which stopped growth approximately 12 days after inoculation. Single trichome cells, invaded by fungal hyphae at various fungal infection stages during the 12-day period after the inoculation of single conidia, were cut at the bases and directly collected with small precision scissors (i.e., microscissors) held by the manipulator under a DM. Subsequently, suc-polymerase chain reaction (PCR) (reverse transcription (RT)-PCR followed by nested (N)-PCR) was conducted to explore gene expression in the infected trichome. We selected intron-containing genes from tomatoes and powdery mildew fungi for the detection of constitutive gene transcripts, namely plasma membrane H+-ATPase (LHA2) and β-tubulin 2 (TUB2) genes. In suc-PCR, a single band from spliced mRNAs of both LHA2 and TUB2 genes were detected, suggesting that both genes were successfully transcribed in single KTP-03-infected trichomes. With combined primers for both LHA2 and TUB2 (multiplex RT-PCR/N-PCR), two bands were detected through the amplification of intron-spliced mRNAs of both genes. Therefore, our single-trichome cell PCR amplification method is effective for detecting the expression patterns of genes from both tomato and powdery mildew fungus. Combinations of digital microscopy, microscissors, and multiplex RT-PCR/N-PCR amplification techniques will be useful for simultaneously analysing the molecular interactions between plants and powdery mildew fungi at the level of single tomato leaf trichome cells. Also, this employed technique will be of benefit in other plant species and crops, possessing leaf trichome cells, to elucidate the molecular interactions between plants and pathogens.
  • Tomoko Suzuki; Shota Iwasaki; Hatsune Hisazumi; Ayumi Miyamoto; Hayato Ogami; Yoshihiro Takikawa; Koji Kakutani; Yoshinori Matsuda; Teruo Nonomura
    Agriculture MDPI AG 12 (2) 198 - 198 2022/01 
    Powdery mildew fungi infect plant leaves, reducing the yield of infected melon plants. Therefore, an eco-friendly method of controlling powdery mildew in melon plants needs to be developed. A previous study described how the morphological characteristics of the conidiophores of the melon powdery mildew fungus Podosphaera xanthii Pollacci (designated KMP-6N) grown under greenhouse (natural) conditions and red light-emitting diode (LED) irradiation differed from those grown under growth chamber conditions and blue LED irradiation. In the present study, conidiophores with unconstricted conidia under blue light were collected and inoculated onto host leaves through micromanipulation; the unconstricted conidia germinated and infected the leaves, producing vigorously elongated hyphae. The number of conidia collected, the initial times of conidial release from single colonies, and the number of conidia remaining in chains on conidiophores were examined with electrostatic techniques. Under red light, the number of collected conidia gradually increased with the light irradiation period. The initial conidial release occurred between 2 to 4 h; the number of conidia remaining on the conidiophores gradually decreased and, eventually, the conidiophore lengths became shorter. In contrast, under blue light, few conidia were collected at any given time; the number of conidia on the conidiophores gradually increased and, eventually, the conidiophore lengths became longer. Next, the effects of red and blue light on the spread of powdery mildew infection by placing a KMP-6N-infected melon seedling at the centre of a tray containing healthy melon seedlings were examined. Almost all healthy seedlings caused powdery mildew symptoms at ca. 21 days after red light irradiation, whereas only healthy seedlings near the infected seedlings showed symptoms after blue light irradiation. Thus, the spread of melon powdery mildew infection clearly differed between red and blue light irradiation. This is the first report describing the effects of red and blue light on the spread of P. xanthii infection from a single infected seedling to healthy host seedlings; their results provide insight into the ecological mechanisms of powdery mildew conidial scatter from conidiophores.
  • S. Iwasaki; S. Asano; K. Yoshida; S. Kitamura; A. Taira; Y. Honda; T. Suzuki; Y. Takikawa; K. Kakutani; Y. Matsuda; T. Nonomura
    Australasian Plant Pathology Springer Science and Business Media LLC 50 (5) 571 - 587 0815-3191 2021/09
  • Koji Kakutani; Yoshihiro Takikawa; Yoshinori Matsuda
    Insects MDPI AG 12 (6) 522 - 522 2021/06 [Refereed]
     
    We developed an arc discharge exposer (ADE) that kills rice weevils nesting in dried rice. The ADE features multiple identical metal plates, half of these are linked to a voltage generator and the others are grounded. The plates were arrayed in parallel and an electric field formed between them. Any insect entering the field was arced from the negatively charged plate and killed. The ADE was placed on a vessel containing pest-infested rice grains; the insects were lured out of the grains by mechanically vibrating the vessel. When rice grains move, insects tend to climb upward, thus, the weevils were effectively removed. Our electrostatic apparatus is easy to construct and could be used to control pests in stored rice.
  • Koji Kakutani; Yoshinori Matsuda; Teruo Nonomura; Yoshihiro Takikawa; Takeshi Takami; Hideyoshi Toyoda
    International Journal of Environmental Research and Public Health MDPI AG 18 (9) 4934 - 4934 2021/05 [Refereed]
     
    The purpose of this study was to develop a simple electrostatic apparatus to precipitate virus particles spread via droplet transmission, which is especially significant in the context of the recent coronavirus disease 2019 (COVID-19) pandemic. The bacteriophage φ6 of Pseudomonas syringae was used as a model of the COVID-19 virus because of its similar structure and safety in experiments. The apparatus consisted of a spiked, perforated stainless plate (S-PSP) linked to a direct-current voltage generator to supply negative charge to the spike tips and a vessel with water (G-water) linked to a ground line. The S-PSP and G-water surface were paralleled at a definite interval. Negative charge supplied to the spike tips positively polarised the G-water by electrostatic induction to form an electric field between them in which ionic wind and negative ions were generated. Bacteriophage-containing water was atomised with a nebuliser and introduced into the electric field. The mist particles were ionised by the negative ions and attracted to the opposite pole (G-water). This apparatus demonstrated a prominent ability to capture phage-containing mist particles of the same sizes as respiratory droplets and aerosols regardless of the phage concentration of the mist particles. The trapped phages were successfully sterilised using ozone bubbling. Thus, the present study provides an effective system for eliminating droplet transmission of viral pathogens from public spaces.
  • Koji Kakutani; Yoshinori Matsuda; Teruo Nonomura; Yoshihiro Takikawa; Kazumi Osamura; Hideyoshi Toyoda
    Agriculture MDPI AG 11 (2) 176 - 176 2021/02 
    The purpose of the study was to construct an electrostatic insect-capturing apparatus that could be applied to a drone (quadcopter). For this purpose, a double-charged dipolar electric field screen (DD-screen) was constructed using oppositely charged insulator tubes that was then attached to a drone. For charging, the inner surface of the tubes was coated with a conductive paste and then linked to a negative or positive voltage generator. The opposite charges of the tubes formed an electric field between them and created an attractive force to capture insects that entered the field. The DD-screen constructed here was sufficiently light to enable its attachment to a drone. The screen was hung from the drone perpendicular to the direction of drone movement, so as to receive the longitudinal airflow produced by the movement of the drone. It was positioned 1.8 m below the drone body to avoid the influence of the downward slipstream generated by the rotating propellers. Eventually, the drone was able to conduct a stable flight, with sufficient endurance, and captured airborne insects carried by an airflow of 8 m/s during the flight. This study, therefore, provides an experimental basis for establishing a new method for conducting trap-based monitoring of airborne insects during remote-controlled flight through operation of a DD-screen attached to a drone.
  • Márk Z Németh; Yuusaku Mizuno; Hiroki Kobayashi; Diána Seress; Naruki Shishido; Yutaka Kimura; Susumu Takamatsu; Tomoko Suzuki; Yoshihiro Takikawa; Koji Kakutani; Yoshinori Matsuda; Levente Kiss; Teruo Nonomura
    PloS one 16 (5) e0251444  2021 [Refereed]
     
    A total of 26 Ampelomyces strains were isolated from mycelia of six different powdery mildew species that naturally infected their host plants in Japan. These were characterized based on morphological characteristics and sequences of ribosomal DNA internal transcribed spacer (rDNA-ITS) regions and actin gene (ACT) fragments. Collected strains represented six different genotypes and were accommodated in three different clades of the genus Ampelomyces. Morphology of the strains agreed with that of other Ampelomyces strains, but none of the examined characters were associated with any groups identified in the genetic analysis. Five powdery mildew species were inoculated with eight selected Ampelomyces strains to study their mycoparasitic activity. In the inoculation experiments, all Ampelomyces strains successfully infected all tested powdery mildew species, and showed no significant differences in their mycoparasitic activity as determined by the number of Ampelomyces pycnidia developed in powdery mildew colonies. The mycoparasitic interaction between the eight selected Ampelomyces strains and the tomato powdery mildew fungus (Pseudoidium neolycopersici strain KTP-03) was studied experimentally in the laboratory using digital microscopic technologies. It was documented that the spores of the mycoparasites germinated on tomato leaves and their hyphae penetrated the hyphae of Ps. neolycopersici. Ampelomyces hyphae continued their growth internally, which initiated the atrophy of the powdery mildew conidiophores 5 days post inoculation (dpi); caused atrophy 6 dpi; and complete collapse of the parasitized conidiphores 7 dpi. Ampelomyces strains produced new intracellular pycnidia in Ps. neolycopersici conidiophores ca. 8-10 dpi, when Ps. neolycopersici hyphae were successfully destroyed by the mycoparasitic strain. Mature pycnidia released spores ca. 10-14 dpi, which became the sources of subsequent infections of the intact powdery mildew hyphae. Mature pycnidia contained each ca. 200 to 1,500 spores depending on the mycohost species and Ampelomyces strain. This is the first detailed analysis of Ampelomyces strains isolated in Japan, and the first timing and quantification of mycoparasitism of Ps. neolycopersici on tomato by phylogenetically diverse Ampelomyces strains using digital microscopic technologies. The developed model system is useful for future biocontrol and ecological studies on Ampelomyces mycoparasites.
  • Yoshinori Matsuda; Yoshihiro Takikawa; Koji Kakutani; Teruo Nonomura; Kiyotsugu Okada; Shin-ichi Kusakari; Hideyoshi Toyoda
    Agriculture 10 600 1 - 600 12 2020/12 [Refereed]
  • Yoshihiro Takikawa; Takeshi Takami; Koji Kakutani
    Insects MDPI AG 11 (9) 561 - 561 2020/08 [Refereed]
     
    In the present study, the relationship between body water loss and conductivity was examined in adult houseflies (Musca domestica). The events an insect experiences in an electric field are caused by the conductive nature of the insect body (i.e., movement of electricity within or its release from the insect). After houseflies were dehydrated, rehydrated, refrigerated, and frozen and thawed, they were placed in static and dynamic electric fields. Untreated houseflies were deprived of their free electrons to become positively charged and then attracted to the insulated negative pole in the static electric field and were exposed to corona and arc discharge from non-insulated negative pole in the dynamic electric field. There was no current in the bodies of dehydrated and frozen flies; hence, there was no attractive force or discharge exposure. In the remaining insects, the results were identical to those in the untreated control insects. These results indicated that the reduction of body water conductivity inhibited the release of electricity from the body in the static electric field and the discharge-mediated current flow through the body in the dynamic electric field. The insect was affected by the electric fields because of its conductivity mediated by body water.
  • T. Suzuki; Y. Nakao; R. Ura; M. Nakaba; T. Nishiyama; Y. Takikawa; Y. Matsuda; K. Kakutani; Y. Bai; T. Nonomura
    Australasian Plant Pathology Springer Science and Business Media LLC 49 (4) 451 - 460 0815-3191 2020/07 [Refereed]
  • Yoshinori Matsuda; Yoshihiro Takikawa; Koji Kakutani; Teruo Nonomura; Hideyoshi Toyoda
    Insects MDPI AG 11 (3) 187 - 187 2020/03 [Refereed]
     
    The present study was conducted to establish an electrostatic-based experimental system to enable new investigations of insect behavior. The instrument consists of an insulated conducting copper ring (ICR) linked to a direct current voltage generator to supply a negative charge to an ICR and a grounded aluminum pole (AP) passed vertically through the center of the horizontal ICR. An electric field was formed between the ICR and the AP. Rice weevil (Sitophilus oryzae) was selected as a model insect due to its habit of climbing erect poles. The electric field produced a force that could be imposed on the insect. In fact, the negative electricity (free electrons) was forced out of the insect to polarize its body positively. Eventually, the insect was attracted to the oppositely charged ICR. The force became weaker on the lower regions of the pole; the insects sensed the weaker force with their antennae, quickly stopped climbing, and retraced their steps. These behaviors led to a pole-ascending–descending action by the insect, which was highly reproducible and precisely corresponded to the changed expansion of the electric field. Other pole-climbing insects including the cigarette beetle (Lasioderma serricorne), which was shown to adopt the same behavior.
  • Yoshihiro Takikawa; Yoshinori Matsuda; Teruo Nonomura; Koji Kakutani; Shin-ichi Kusakari; Hideyoshi Toyoda
    Journal of Agricultural Science 12 (2) 50 - 60 1916-9752 2020/01 [Refereed]
  • Takikawa Y; Kakutani K; Matsuda Y; Nonomura T; Kusakari S; Toyoda H
    Journal of Agricultural Science 11 (18) 1 - 20 1916-9752 2019/11 [Refereed]
  • T. SuzukiR; NakamuraN; TakagiY. TakikawaK; KakutaniY. MatsudaK; MatsuiT. Nonomura
    Australasian Plant Pathology 48 (3) 297 - 307 2019/05 [Refereed]
  • Yoshinori Matsuda; Koji Kakutani; Teruo Nonomura; Yoshihiro Takikawa; Kiyotsugu Okada; Manabu Shibao; Kazuhiro Miyama; Shinya Yokoo; Shin-ichi Kusakari; Hideyoshi Toyoda
    Instruments MDPI AG 2 (3) 13 - 13 2018/07 [Refereed]
     
    An electrostatic apparatus was constructed to capture tobacco sidestream smoke. This apparatus consisted of a perforated polypropylene plate with metal spikes and a grounded metal net arrayed in parallel at a defined interval. Spikes were negatively charged to positively polarize the net and an electric field was formed between the opposite charges of the spike tips and the grounded net. Discharge from the spike tips occurred, which depended on the pole distance and the voltage applied to the spikes. At lower voltages (<12.1 kV) that do not cause arc discharge from the tips, a corona discharge occurred with the generation of an ionic wind from the spiked plate to the net. This discharge increased in direct proportion to the applied voltage and relative humidity, while a larger corona discharge generated a stronger ionic wind. The ionic wind involved negative ions and the number of negative ions in the wind increased with increasing applied voltage. The optimal voltage (10 kV) generated sufficient negative ions to ionize smoke particles in the electric field, before the ionized smoke particles were successfully captured by the oppositely charged metal net. Thus, this study provides an experimental basis for the practical application of an electrostatic-based method to prevent the production of tobacco sidestream smoke that leads to passive smoking by non-smokers.
  • Electrocution of mosquitoes in a piggery by a novel electrostatic window screen to minimize mosquito transmission of Japanese encephalitis virus
    Kakutani K; Matsuda Y; Takikawa Y; Nonomura T; Okada K; Shibao M; Kusakari S; Miyama K; Toyoda H
    International Journal of Scientific Research 7 (5) 47 - 50 2018/05 [Refereed]
  • Selective electrostatic eradication of Sitophilus oryzae nesting in stored rice.
    Yoshinori Matsuda; Yoshihiro Takikawa; Teruo Nonomura; Koji Kakutani; Kiyotsugu Okada; Manabu Shibao; Shin-ichi Kusakari; Kazuhiro Miyama; Hideyoshi Toyoda
    Journal of Food Technology and Preservation 2 (1) 15 - 20 2018/04 [Refereed]
  • Tomoko Suzuki; Shougo Nishimura; Kazuhiro Yagi; Ryousuke Nakamura; Yoshihiro Takikawa; Yoshinori Matsuda; Koji Kakutani; Teruo Nonomura
    PHYTOPARASITICA SPRINGER 46 (1) 31 - 43 0334-2123 2018/02 [Refereed]
     
    The lengths of conidiophores in fungal colonies of the melon powdery mildew pathogen Podosphaera xanthii Pollacci KMP-6 N cultured under greenhouse (natural) conditions differed markedly from those cultured in a growth chamber. We hypothesized that light wavelength was responsible for the differences in conidiophore length. In this study, we examined the effects of light-emitting diode (LED) irradiation (purple, blue, green, orange, and red light) and white light on colony development and conidiophore formation in KMP-6 N using a stereomicroscope and a high-fidelity digital microscope. Colonies on leaves were flat under greenhouse conditions and under red LED light irradiation but were stacked under growth chamber conditions and under purple, blue, green, and orange LED light irradiation. In addition, KMP-6 N formed catenated conidia comprising six conidia per conidiophore under greenhouse conditions and red light but more than seven conidia per conidiophore under growth chamber conditions and purple, blue, green, and orange light. Furthermore, almost none of the conidia on top of the conidiophores grown under blue light were fully constricted. Therefore, these fungi could not scatter their conidia and spread infection. This is the first report of the effects of LED lights on conidiophore formation in the melon powdery mildew fungus P. xanthii. The results provide insight into the mechanisms underlying the responses of conidiophores to light of specific wavelengths and conidial scatter from conidiophores of melon powdery mildew fungi.
  • Tomoko Suzuki; Tomoe Murakami; Yoshihiro Takizumi; Hiroyuki Ishimaru; Daiki Kudo; Yoshihiro Takikawa; Yoshinori Matsuda; Koji Kakutani; Yuling Bai; Teruo Nonomura
    EUROPEAN JOURNAL OF PLANT PATHOLOGY SPRINGER 150 (1) 115 - 125 0929-1873 2018/01 [Refereed]
     
    The present study aimed to explore the possibility of using the type I trichomes of tomato (Solanum lycopersicum) to monitor the infection processes of powdery mildews by microscopy. Individual trichome cells of two tomato genotypes were inoculated with pathogenic and non-pathogenic powdery mildew species, Pseudoidium neolycopersici, Erysiphe trifoliorum and Podosphaera xanthii. On the trichome cells of the tomato cultivar Moneymaker, hyphae of the pathogenic Pseudoidium neolycopersici (isolates KTP-03 and KTP-04) grew vigorously; whereas hyphal growth of the non-pathogenic Erysiphe trifoliorum and Podosphaera xanthii ceased after appressorium formation, which was associated with papilla formation and hypersensitive cell death, respectively. Similar infection processes of the tested powdery mildews were seen in Moneymaker epidermal cells. Therefore, tomato trichomes are suitable for analysing, at individual cell level, the infection processes of different pathotypes of powdery mildews and for observing the cytological responses of plants by non-pathogenic powdery mildews. On the other hand, it was observed that both isolates KTP-03 and KTP-04 failed to produce conidiophores on the hyphae elongating on Moneymaker trichomes. Similarly, no conidiophores were produced on the hyphae elongating on trichomes of Solanum peruvianum LA2172, which is resistant to KTP-03 and susceptible to KTP-04. Interestingly, delayed cell death occurred in LA2172 epidermal cells, which were attacked by KTP-03 hyphae elongating from trichomes and conidiophores were formed on new hyphae growing from the leaf epidermal cells. Thus, leaf trichomes and epidermal cells of the wild tomato species LA2172 reacted differently to the avirulent isolate KTP-03.
  • Successful single-truss cropping cultivation of healthy tomato seedlings raised in an electrostatically guarded nursery cabinet with non-chemical control of whiteflies.
    Kakutani K; Matsuda Y; Nonomura T; Takikawa Y; Okada K; Shibao M; Kusakari S; Toyoda H
    Global Journal of Pests, Diseases and Crop Protection 5 (4) 269 - 275 2017/07 [Refereed]
  • Electrostatic elimination of fine smoke particles by a newly devised air purification screen
    Takikawa Y; Matsuda Y; Nonomura T; Kakutani K; Kusakari S; Toyoda H
    International Journal of Scientific Research in Agricultural Sciences 5 (1) 17 - 21 2017/02 [Refereed]
  • Yoshihiro Takikawa; Yoshinori Matsuda; Teruo Nonomura; Koji Kakutani; Shin-Ichi Kusakari; Hideyoshi Toyoda
    International Journal of Environmental Research and Public Health MDPI AG 14 (1) 82 - 82 1660-4601 2017/01 [Refereed]
     
    An electrostatic-barrier-forming window (EBW) was devised to capture airborne pollen, which can cause allergic pollinosis. The EBW consisted of three layers of insulated conductor wires (ICWs) and two voltage generators that supplied negative charges to the two outer ICW layers and a positive charge to the middle ICW layer. The ICWs generated an attractive force that captured pollen of the Japanese cedar, Cryptomeria japonica, from air blown through the EBW. The attractive force was directly proportional to the applied voltage. At >= 3.5 kV, the EBW exerted sufficient force to capture all pollen carried at an air flow of 3 m/s, and pollen-free air passed through the EBW. The findings demonstrated that the electrostatic barrier that formed inside the EBW was very effective at capturing airborne pollen; thus, it could allow a home to remain pollen-free and healthy despite continuous pollen exposure.
  • Elimination of whiteflies colonising greenhouse tomato plants using an electrostatic flying insect catcher.
    Takikawa Y; Matsuda Y; Nonomura T; Kakutani K; Okada K; Shibao M; Kusakari S; Toyoda H
    International Journal of Current Advanced Research 6 (8) 5517 - 5521 2017 [Refereed]
  • T. Nonomura; Y. Matsuda; H. Toyoda; Y. Takikawa; K. Kakutani
    INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY AND OTHER OMICS IN VEGETABLE SCIENCE INT SOC HORTICULTURAL SCIENCE 1145 31 - 37 0567-7572 2016 [Refereed]
     
    Single-cell polymerase chain reaction (PCR) was conducted to detect in situ gene expression in targeted cells of tomato leaf trichomes. The cytoplasm was removed with a micropipette under a light microscope and subsequently used for reverse transcription PCR (RT-PCR), followed by nested PCR. Two intron-containing genes, a glyceraldehyde 3-phosphate dehydrogenase gene and a plasma membrane H+-ATPase gene, were constantly expressed in these cells and therefore used as indicators of successful PCR reactions. In addition, the use of nucleus-free cellular contents for the RT-PCR and subsequent nested PCR analyses was effective for preventing contamination with the products derived from misamplification of corresponding genomic DNA sequences. Using this method, we detected the expression of certain stimuli-activated genes, following the exposure of trichome cells to volatile chemicals. Therefore, the present technique can be used to directly detect gene expression in single trichome cells of tomato leaves in response to external stimulation.
  • Y. Takikawa; H. Toyoda; Y. Matsuda; T. Nonomura; K. Kakutani
    INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY AND OTHER OMICS IN VEGETABLE SCIENCE INT SOC HORTICULTURAL SCIENCE 1145 23 - 29 0567-7572 2016 [Refereed]
     
    A microscopy-based needle micromanipulation technique for direct injection of foreign material into target cells was refined as a novel method of aspiration of cellular contents from a single target cell using a micropipette. Subsequently, we performed direct PCR amplification of mRNAs transcribed in target tomato callus cells. Friable calli were induced from leaf explants, and single cells from active small cell aggregates were used as targets for aspiration. The aspirated cellular contents were subjected to RT-PCR and subsequent nested PCR amplification analyses. Five tomato genes (LHA2, GAPDH, CHI3, PI2 and TLC1) were selected from the cDNA database as PCR targets. The GAPDH and LHA2 genes were amplified from all aspirated samples and were used as indicators of successful PCR amplification. Aspiration of only the cytosol (excluding the nucleus), facilitated amplification of mature target gene mRNA that was not contaminated with PCR products derived from the genomic DNA sequences of the target genes. We identified novel stimulus activated genes, such as CHI3 and TLC1, which were constitutively transcribed in tomato callus cells.
  • K. Kakutani; Y. Matsuda; T. Nonomura; H. Toyoda; Y. Takikawa
    INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY AND OTHER OMICS IN VEGETABLE SCIENCE INT SOC HORTICULTURAL SCIENCE 1145 (1145) 17 - 22 0567-7572 2016 [Refereed]
     
    This study examined whether Agrobacterium-mediated T-DNA insertion was useful for obtaining mutant melon plants. For this purpose, we used Agrobacterium rhizogenes because of its frequent hairy root production when inoculated into plants and the easy detection of fruit odours produced by the hairy roots. Of the 6534 hairy root clones obtained, five clones were mutants emitting fruit odours, and the KMH-4196 clone produced the strongest melon fruit odours. These odours were due to the production of at least four compounds that were not detected in non-aromatic hairy root clones: (Z)-3-hexenol, (E)-2-hexenal, 1-nonanoland (Z)-6-nonenol. (Z)-6-nonenol was the most important and was produced stably during successive subculture for 3 years. Molecular analyses indicated that a single copy of T-DNA from the inoculated bacteria was integrated into a chromosome in the clone, suggesting that Agrobacterium-mediated T-DNA insertion is an effective mutagenesis method in higher plants.
  • An electrostatic nursery shelter for raising pest and pathogen free tomato seedlings in an open-window greenhouse environment
    Takikawa Y; Matsuda Y; Nonomura T; Kakutani K; Okada K; Morikawa S; Shibao M; Kusakari S; Toyoda H
    Journal of Agricultural Science 8 13 - 25 2016 [Refereed]
  • Yoshihiro Takikawa; Satomi Kida; Fuji Asayama; Teruo Nonomura; Yoshinori Matsuda; Koji Kakutani; Hideyoshi Toyoda
    JOURNAL OF BRYOLOGY TAYLOR & FRANCIS LTD 37 (3) 250 - 250 0373-6687 2015/09 [Refereed]
  • Yoshihiro Takikawa; Teruo Nonomura; Shouta Miyamoto; Naoki Okamoto; Tomoe Murakami; Yoshinori Matsuda; Koji Kakutani; Shin-ichi Kusakari; Hideyoshi Toyoda
    PHYTOPARASITICA SPRINGER 43 (4) 517 - 530 0334-2123 2015/09 [Refereed]
     
    Melons (Cucumis melo L.) grown hydroponically in a greenhouse were heavily infested with powdery mildew. We isolated powdery mildew pathogens from the melon leaves and identified the isolate as Podosphaera xanthii KMP-6N, based on morphological characteristics and sequences of ribosomal DNA internal transcribed spacer (rDNA-ITS) regions. Host ranges of KMP-6N were determined by estimating the infectivity or pathogenicity after inoculating the conidia onto multiple plant species. The fungi caused severe powdery mildew symptoms on Cucurbitaceae plants, producing scattered conidia on conidiophores. The goal of this study was to observe KMP-6N conidiogenesis on melon leaves. The pathogen formed completely catenated conidiophores approximately 24 h from conidiophore erection to release of mature conidia. Six conidia were produced on the conidiophores and only the conidia at the apex reached maturity. The cycles of conidial release were repeated on melon leaves 14 to 18 times, at approximately 6-h intervals. In the final stage, conidia were released without causing growth and septation of generative cells. Conidiophores produced an average of 36 conidia during a 90-h period. In our study, the modes of conidiogenesis, lifetime of conidiophores and productivity of conidia on a conidiophore were described for powdery mildew fungi.
  • 中村恭子; 赤木淳二; 赤木淳二; 石伏史明; 谷恭輔; 森川敏生; 森川敏生; YUTANA Pongpiriyadacha; 村岡修; 村岡修; 村岡修; 早川尭夫; 角谷晃司; 角谷晃司
    生薬学雑誌 日本生薬学会 69 (2) 53 - 58 1349-9114 2015/08 [Refereed]
     
    Genus Salacia (Hippocrateaceae) contains woody climbing plants widely distributed in India, Sri Lanka, China, and Southeast Asian countries. The stems and roots of some species of the plant have traditionally been used as a specific remedy for early-stage diabetes in Ayurvedic medicine. Recently, a variety of health foods and beverages made from the extracts of Salacia genus plants claiming the prevention and/or control of diabetes has been developed in Japan and North America. In order to distinguish and identify the species of the genus Salacia plants genetically, DNA sequences in the internal transcribed spacer (ITS) region of the nuclear ribosomal RNA gene (nrDNA) with respect to three species of the plant, i.e., S. reticulata, S. oblonga, and S. chinensis, collected in Sri Lanka, India and Thailand were analyzed and compared with each other. Among them a characteristic feature discriminable from the other two species was observed with respect to the DNA sequence of the samples identified as S. chinensis. On the other hand, no distinct difference or character which led to the identification of S. reticulata or S. oblonga was observed in the present study.
  • 中村恭子; 大野加奈; 森健太郎; 瀧川義浩; 角谷晃司
    薬用植物研究 37 (1) 22 - 28 2434-7353 2015/06 [Refereed]
  • Yoshihiro Takikawa; Yoshinori Matsuda; Koji Kakutani; Teruo Nonomura; Shin-ichi Kusakari; Kiyotsugu Okada; Junji Kimbara; Kazumi Osamura; Hideyoshi Toyoda
    INSECTS MDPI 6 (2) 442 - 454 2075-4450 2015/06 [Refereed]
     
    Our greenhouse tomatoes have suffered from attacks by viruliferous whiteflies Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) over the last 10 years. The fundamental countermeasure was the application of an electric field screen to the greenhouse windows to prevent their entry. However, while the protection was effective, it was incomplete, because of the lack of a guard at the greenhouse entrance area; in fact, the pests entered from the entrance door when workers entered and exited. To address this, we developed a portable electrostatic insect sweeper as a supplementary technique to the screen. In this sweeper, eight insulated conductor wires (ICWs) were arranged at constant intervals along a polyvinylchloride (PVC) pipe and covered with a cylindrical stainless net. The ICWs and metal net were linked to a DC voltage generator (operated by 3-V alkaline batteries) inside the grip and oppositely electrified to generate an electric field between them. Whiteflies on the plants were attracted to the sweeper that was gently slid along the leaves. This apparatus was easy to operate on-site in a greenhouse and enabled capture of the whiteflies detected during the routine care of the tomato plants. Using this apparatus, we caught all whiteflies that invaded the non-guarded entrance door and minimized the appearance and spread of the viral disease in tomato plants in the greenhouse.
  • Yoshihiro Takikawa; Satomi Kida; Fuji Asayama; Teruo Nonomura; Yoshinori Matsuda; Koji Kakutani; Hideyoshi Toyoda
    JOURNAL OF BRYOLOGY MANEY PUBLISHING 37 (1) 1 - 7 0373-6687 2015/03 [Refereed]
     
    We examined the defensive responses of leaf surfaces, rhizoids, and protonemata of the moss Aphanoregma patens (Hedw.) Lindb. to inoculation with zoospores and encysted zoospores (cysts) of the oomycete Pythium aphanidermatum OPU849. Aphanoregma patens infected with Pythium exhibited extensive browning of the leaves and stems 4 days after inoculation. The zoospore infection sites were rhizoids and protonemata. Cysts on the rhizoids and protonemata germinated and formed elongated hyphae that invaded the cells of the moss, forming oospores and lobed sporangia. The subsequent production of new zoospores on infected plants indicates that A. patens is a suitable host for manipulating the life cycle of Pythium. However, cysts inoculated onto leaf surfaces did not germinate, and they began to show signs of disruption after only 3 hours. After 2 days, more than 80% of the cysts on leaf surfaces were completely disrupted, possibly due to the secretion of degrading enzymes. Cyst disruption was not observed on rhizoids nor on protonemata.
  • Development of an electrostatic trap with an insect discharge recorder for multiple real-time monitoring of pests prowling in a warehouse
    Takikawa Y; Matsuda Y; Nonomura T; Kakutani K; Kusakari S; Toyoda H
    International Journal of Advance Agricultural Research 3 55 - 63 2015 [Refereed]
  • Y. Matsuda; T. Nonomura; K. Kakutani; J. Kimbara; K. Osamura; S. Kusakari; H. Toyoda
    ELECTROSTATICS 2015 IOP PUBLISHING LTD 646 12003  1742-6588 2015 [Refereed]
     
    An electric field screen is a physical device used to exclude pest insects from greenhouses and warehouses to protect crop production and storage. The screen consists of iron insulated conductor wires (ICWs) arrayed in parallel and linked to each other, an electrostatic DC voltage generator used to supply a negative charge to the ICWs, and an earthed stainless net placed on one side of the ICW layer. The ICW was negatively charged to polarize the earthed net to create a positive charge on the ICW side surface, and an electric field formed between the opposite charges of the ICW and earthed net. The current study focused on the ability of the screen to repel insects reaching the screen net. This repulsion was a result of the insect's behaviour, i.e., the insects were deterred from entering the electric field of the screen. In fact, when the screen was negatively charged with the appropriate voltages, the insects placed their antennae inside the screen and then flew away without entering. Obviously, the insects recognized the electric field using their antennae and thereby avoided entering. Using a wide range of insects and spiders belonging to different taxonomic groups, we confirmed that the avoidance response to the electric field was common in these animals.
  • Y. Matsuda; K. Kakutani; T. Nonomura; J. Kimbara; K. Osamura; S. Kusakar; H. Toyoda
    ELECTROSTATICS 2015 IOP PUBLISHING LTD 646 12002  1742-6588 2015 [Refereed]
     
    An electric field screen can be used to keep mosquitoes out of houses with open windows. In this study, doubly charged dipolar electric field screens (DD-screens) were used to capture mosquitoes entering through a window. The screen had two components: three layers of insulated conductor iron wires (ICWs) in parallel arrays and two electrostatic direct current (DC) voltage generators that supplied negative or positive voltages to the ICWs. Within each layer, the ICWs were parallel at 5-mm intervals, and connected to each other and to a negative or positive voltage generator. The negatively and positively charged ICWs are represented as ICW(-) and ICW(+), respectively. The screen consisted of one ICW(+) layer with an ICW(-) layer on either side. The Asian tiger mosquito (Aedes albopictus) and house mosquito (Culex pipiens) were used as models of vectors carrying viral pathogens. Adult mosquitoes were blown into the space between the ICWs by sending compressed air through the tip of an insect aspirator to determine the voltage range that captured all of the test insects. Wind speed was measured at the surface of the ICW using a sensitive anemometer. The result showed that at >= 1.2 kV, the force was strong enough that the ICWs captured all of the mosquitoes, despite a wind speed of 7 m/s. Therefore, the DD-screen could serve as a physical barrier to prevent noxious mosquitoes from entering houses with good air penetration.
  • Yoshihiro Takikawa; Yoshinori Matsuda; Teruo Nonomura; Koji Kakutani; Junji Kimbara; Kazumi Osamura; Shin-ichi Kusakari; Hideyoshi Toyoda
    AEROBIOLOGIA SPRINGER 30 (4) 435 - 444 0393-5965 2014/12 [Refereed]
     
    Old books are highly susceptible to mould infection, and an effective method for avoiding moulding is needed to safely preserve valuable books in library stack rooms. Guarding a bookshelf with an electric field screen is a physical method that prevents airborne spores from entering the space used for book preservation. In this study, insulated conductor wires (ICWs) were used as electrodes to form electric fields. The ICWs were arrayed in parallel and linked to each other and to a direct current voltage generator. The electric field screen consisted of two layers of ICWs, which were negatively and positively charged with equal voltages to make dipoles, ICW(-) and ICW(+). Both ICWs generated an attractive force that captured airborne spores of Penicillium digitatum that were blown inside the screen. The attractive force was directly proportional to the applied voltage. At a parts per thousand 0.9 kV, the screen exerted sufficient force to capture all airflow-carried spores, but a few spores that were once captured were repulsed out of the electric field when subsequent spores were attracted to positions proximal to them. This phenomenon was explained by creeping discharge between spores located close to each other on the ICW surface. This spore-repulsion problem was resolved by adding an additional ICW layer to the electric field screen, namely an electric field screen with an ICW(-) layer on both sides of an ICW(+) layer. The present study demonstrated that the three-layered electric field screen remained mould-free inside a screen-guarded bookshelf, irrespective of continuous spore exposure.
  • Teruo Nonomura; Yoshinori Matsuda; Koji Kakutani; Yoshihiro Takikawa; Junji Kimbara; Kazumi Osamura; Shin-ichi Kusakari; Hideyoshi Toyoda
    Journal of Agricultural Science Canadian Center of Science and Education 6 (12) 1916-9752 2014/11 [Refereed]
  • Teruo Nonomura; Yoshinori Matsuda; Koji Kakutani; Junji Kimbara; Kazumi Osamura; Shin-ichi Kusakari; Hideyoshi Toyoda
    JOURNAL OF ELECTROSTATICS ELSEVIER SCIENCE BV 72 (1) 1 - 5 0304-3886 2014/02 [Refereed]
     
    A simple electrostatic apparatus was devised to measure dischargeable electricity and bioelectric potentials produced by flies. The apparatus involved two insulated electrodes, ICW(-) and ICW(+), oppositely charged with equal voltages supplied by two voltage-generators. In the electric field, the flies became net positive by instantaneously discharging their electricity and were attracted to negative surface charges on ICW(-). The tail-lifting movement by the attracted insect was an action creating electric potentials that could cause discharge of ICW(-). The discharge transiently appeared in response to individual movements and was larger when the tail was lifted at higher angles. (C) 2013 Elsevier B.V. All rights reserved.
  • Y. Takikawa; Y. Senga; T. Nonomura; Y. Matsuda; K. Kakutani; H. Toyoda
    PLANT BIOLOGY WILEY-BLACKWELL 16 (1) 291 - 295 1435-8603 2014/01 [Refereed]
     
    In this study, we observed the germination behaviour of airborne conidia from powdery mildews that settle on thalloid surfaces. We inoculated thalli (flat, sheet-like leaf tissues) and gemmae (small, flat, sheet-like leaf tissues that propagate asexually via bud-like structures) of the common liverwort (Marchantia polymorpha) with conidia from tomato powdery mildew (Oidium neolycopersici; KTP-02) and red clover powdery mildew (Erysiphe trifoliorum; KRCP-4N) and examined their germination and subsequent appressorium formation under a high-fidelity digital microscope. Conidial bodies and germ tubes of the inoculated KRCP-4N conidia were destroyed on both the thalli and gemmae. The destruction of these fungal structures was observed only for KRCP-4N conidia inoculated onto M.polymorpha on both leaf surfaces. No differences in destruction of the KRCP-4N fungal structures between thalli and gemmae were observed. At 4h post-inoculation, destruction of the germ tube tip was observed when it reached the gemmae leaf surface. At 6h post-inoculation, the conidial bodies and germ tubes were destroyed. In contrast, KTP-02 conidia were not destroyed and formed normal, well-lobed appressoria on the surface of M.polymorpha gemmae.
  • T. Nonomura; Y. Matsuda; Y. Takikawa; K. Kakutani; H. Toyoda
    Ann. Rept. Kansai Pl. Prot 56 17 - 20 2014 [Refereed]
  • Xin Su; Wei-Zhuo Xu; Xin Liu; Rui-Fang Zhuo; Cai-Yun Wang; Xin Zhang; K. Kakutani; Song You
    GENE ELSEVIER SCIENCE BV 520 (2) 139 - 147 0378-1119 2013/05 [Refereed]
     
    We used 2D-PAGE to isolate a light-induced protein (AL-A) that is expressed abundantly in light-growth alfalfa sprouts. The seven amino adds of the N-terminal region of the protein were identified, and we searched for the protein in GenBank using the BLAST program. The results of the homology analysis showed that the amino add sequence of the isolated protein is most similar to one from a pea plastocyanin. To identify the protein, we amplified and sequenced the DNA fragment encoding AL-A from genomic alfalfa DNA. We found that the AL-A gene was highly homologous (90%) to the sequences from the pea plastocyanin via multiple alignments, and the deduced protein precursor was predicted to be chloroplast-specific via the ChloroP computer program. The protein was named alfalfa-plastocyanin (AL-P). It was characterized as being a light-inducible protein, and RT-PCR analysis showed that AL-P mRNA transcription only occurred in the leaves of the alfalfa plant and the alfalfa seedlings growth in lighted conditions. PCR was also used to amplify the DNA fragment encoding the AL-P promoter (AL-Pp) from genomic alfalfa DNA. PlantCARE analysis of the promoter sequence indicated that both a typical TATA box and a CAAT box were located in the promoter sequence, and some of the cis-elements that are responsible for light responsiveness were also identified within this promoter region. The AL-P gene promoter fused to the beta-glucuronidase (GUS) reporter gene has been examined for expression in transgenic alfalfa seedlings. Our findings have a potential application in plant genetic engineering; the AL-Pp may be used to drive the expression of heterologous genes in transgenic alfalfa plants. (c) 2013 Elsevier B.V. All rights reserved.
  • S. Kusakari; H. Toyoda
    近畿大学農学部紀要 近畿大学農学部 46 (46) 55 - 58 0453-8889 2013 
    筆者らは近畿大学農学部(奈良県)においてトマトの一段採り密植栽培を実施しているが，近年，近畿地区においてもトマト黄化葉巻病の発生が問題となってきた。そこで，2007年の栽培から黄化葉巻症状の調査を開始したところ，2009年7月，養液栽培トマトに黄化葉巻の症状が確認され，温室内にタバココナジラミが発生していたことから，トマト黄化葉巻病の可能性が示唆された。本実験では，黄化葉巻の症状を示すトマトからトマト黄化葉巻病ウイルス(TYLCV)の検出を試みるとともに，タバココナジラミのバイオタイプの確認を行ったので報告する。
  • Teruo Nonomura; Yoshinori Matsuda; Shun Yamashita; Haruhiko Akahoshi; Yoshihiro Takikawa; Koji Kakutani; Hideyoshi Toyoda
    PLANT PROTECTION SCIENCE CZECH ACADEMY AGRICULTURAL SCIENCES 49 S33 - S40 1212-2580 2013 [Refereed]
     
    In our routine surveys for the powdery mildew disease in greenhouse tomatoes, we detected a new pathogen that forms pseudochains consisting of 12 conidia. To identify the original plant that dispersed this pathogen, wild plants infected with powdery mildew were monitored. The pathogen on Japanese mallotus, Mallotus japonicus, produced a similar type of pseudochain, and conidia were infectious to tomatoes. Inversely, the conidia on the tomato leaves infected M. japonicus. Infectivity assays and internal transcribed spacer (ITS)-based phylogenetic analyses indicated that the two pathogens on the tomato and M. japonicus were identical. These results suggest that the conidia on M. japonicus can be transmitted to greenhouse tomatoes. This work documents the ecological transmission of conidia between wild plants and greenhouse tomatoes.
  • Teruo Nonomura; Yoshinori Matsuda; Koji Kakutani; Junji Kimbara; Kazumi Osamura; Shin-ichi Kusakari; Hideyoshi Toyoda
    European Journal of Plant Pathology Springer Science and Business Media LLC 134 (4) 661 - 670 0929-1873 2012/12 [Refereed]
     
    Dual functions (insect repelling and capturing) of a single-charged dipolar electric field screen were evaluated to successfully exclude whiteflies from a window-open greenhouse. The screen consisted of three parts: 1) insulated conductor wires (ICWs) arrayed in parallel at 5 mm intervals, 2) two earthed stainless nets placed within 3 mm of both sides of the ICW layer, and 3) a voltage generator for the negatively charged ICWs. The screen formed two electric fields between the ICW-layer and the ICW-side surface of the earthed net and between the ICWs. At negative charging of 1.5-2.5 kV, all whiteflies reaching the outer surface of the screen net avoided entering the electric field and flew away from the screen. This avoidance was disturbed by 3 m s(-1) wind, as the insects were compulsorily blown inside. However, almost all whiteflies (99.4 %) were captured with the ICW. These results indicate that the insect-capturing function is effective to complement a failure to repel. A greenhouse assay was conducted in the screen-attached and non-screened parts in which a greenhouse was divided with a partition. During the 3-month operation, the screen was durable and functional for excluding pests, and better air ventilation changed the climate conditions in the greenhouse. Thus, the present study demonstrated that our electric field screen can provide an airy condition for tomatoes in a window-open greenhouse and successfully exclude whiteflies using dual screen functions.
  • Yoshinori Matsuda; Koji Kakutani; Teruo Nonomura; Junji Kimbara; Shin-ichi Kusakari; Kazumi Osamura; Hideyoshi Toyoda
    JOURNAL OF APPLIED PHYSICS AMER INST PHYSICS 112 (11) 116103  0021-8979 2012/12 [Refereed]
     
    An electric field screen was constructed to examine insect attraction mechanisms in multiple electric fields generated inside the screen. The screen consisted of two parallel insulated conductor wires (ICWs) charged with equal but opposite voltages and two separate grounded nets connected to each other and placed on each side of the ICW layer. Insects released inside the fields were charged either positively or negatively as a result of electricity flow from or to the insect, respectively. The force generated between the charged insects and opposite ICW charges was sufficient to capture all insects. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4767635]
  • Koji Kakutani; Yoshinori Matsuda; Kayo Haneda; Dai Sekoguchi; Teruo Nonomura; Junji Kimbara; Kazumi Osamura; Shin-ichi Kusakari; Hideyoshi Toyoda
    JOURNAL OF ELECTROSTATICS ELSEVIER SCIENCE BV 70 (2) 207 - 211 0304-3886 2012/04 [Refereed]
     
    An insulated conductor wire (ICW) paralleled with an earthed net was used to observe movements by vinegar flies in relation to their electricity release. ICW was negatively charged to create a positive charge on the net. At particular voltages, flies were attracted to ICW. This attraction was triggered by the deprivation of the insect negative charge with the net. Eventually the insects became net positive and were drawn to the ICW negative charge. The attracted insects generated bioelectricity through skeletal muscular movements. However, the electricity produced was depleted by the net without neutralizing their positive charge in the insect body. (c) 2012 Elsevier B.V. All rights reserved.
  • K. Kakutani; Y. Matsuda; T. Nonomura; H. Toyoda; J. Kimbara; K. Osamura; S. Kusakari
    Journal of Agricultural Science Canadian Center of Science and Education 4 (5) 51 - 60 1916-9752 2012/03 [Refereed]
  • K. Kakutani; Y. Matsuda; K. Haneda; T. Nonomura; J. Kimbara; S. Kusakari; K. Osamura; H. Toyoda
    ANNALS OF APPLIED BIOLOGY WILEY-BLACKWELL 160 (3) 250 - 259 0003-4746 2012 [Refereed]
     
    An electric field screen (EF-screen) is a physical device for excluding pest insects from greenhouses and warehouses to protect crops during their production and storage periods. In this study, a simple version of the EF-screen, an insulated conductor iron wire (ICW) paralleled to an earthed net, was constructed to effectively observe the attraction of test insects in relation to their electricity release. The ICW was negatively charged to dielectrically polarise the insulator sleeve of the ICW: negatively on the outer surface and positively on the inner conductor wire surface of the sleeve. The negative surface charge of the ICW caused an electrostatic induction in the earthed net and a resultant positive charge at the ICW-side surface of the net. An electric field formed between the ICW (negative pole) and earthed net (positive pole). Insects were attracted to the ICW when they were placed onto the earthed net. A vital step for the attraction was the creation of a transient bioelectric discharge from an insect. During this discharge, an electric charge of the insect was transferred to the earthed net. Eventually, the insect became net positive and was then attracted to the ICW. The magnitude of the current increased in direct proportion to the increase in voltage applied to the ICW, and the attraction force was directly proportional to the increase in the electric current. Larger voltages were necessary to attract much larger insects because larger insects were stronger and therefore more able to escape from the ICW attraction. Similar results were obtained for a wide range of pest insects belonging to different taxonomic groups (8 orders and 15 families). This study demonstrated that transient bioelectric discharge is common in insects and can be utilised to create an electrostatic force capable of moving insects in a generated electric field.
  • K. Kakutani; Y. Matsuda; T. Nonomura; H. Toyoda; J. Kimbara; S. Kusakari
    INTERNATIONAL SYMPOSIUM ON ADVANCED TECHNOLOGIES AND MANAGEMENT TOWARDS SUSTAINABLE GREENHOUSE ECOSYSTEMS: GREENSYS2011 INT SOC HORTICULTURAL SCIENCE 952 559 - 566 0567-7572 2012 [Refereed]
     
    In an attempt to control insect pests affecting greenhouse tomatoes, we evaluated an electric field screen to create an airy greenhouse condition that successfully excluded insect vectors (whiteflies, green peach aphids, western flower thrips, shore flies) of pathogens. The screen consisted of three parts: 1) insulated conductor wires (ICWs) arrayed in parallel at 5-mm intervals, 2) two stainless-steel nets that were grounded and placed on both sides of the ICWs, and 3) a DC voltage generator to negatively charge the ICWs. An electric field formed between the negative surface charge of the ICWs and the positive charge on the ICW-side surface of the grounded net. The ICWs captured insects that entered the field. Insects that contacted the outer surface of the screen net avoided the electric field and flew away from the screen. During continuous 3-month greenhouse operation, the screen was durable and functional in exerting stable pest exclusion and good air penetration for ventilation under changing greenhouse climate conditions. Thus, our electric field screen provided an airy condition for tomatoes in an open-window greenhouse that successfully excluded flying insect pests.
  • Yoshihiro Takikawa; Koji Kakutani; Yasuo Takigawa
    JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN PHYSICAL SOC JAPAN 80 (9) 95002 - kakutanikoji 0031-9015 2011/09 [Refereed]
  • Yoshihiro Takikawa; Koji Kakutani; Teruo Nonomura; Yoshinori Matsuda; Hideyoshi Toyoda
    MYCOSCIENCE ELSEVIER 52 (3) 204 - 209 1340-3540 2011/05 [Refereed]
     
    In the present study, using a high-fidelity digital microscope, we observed the sequence of appressorial development on the germ tubes of a powdery mildew fungus isolated from red clover leaves. Based on its morphological characteristics and rDNA internal transcribed spacer (ITS) sequences, the fungus was identified as Erysiphe trifoliorum, and one of its isolates, designated as KRCP-4N, was used in this work. The conidial germination of isolate KRCP-4N was studied on host (red clover) and non-host (barley) leaves, as well as on an artificial hydrophobic membrane (Parafilm). More than 90% of conidia germinated synchronously and developed dichotomous appressoria (symmetrical double-headed appressoria) on all substrata used. On host leaves, all appressorium-forming conidia developed hyphae (colony-forming hyphae) from conidial bodies without extending germ tubes from the tips of the appressoria. On non-host leaves and on Parafilm-covered glass slides, however, all conidia extended germ tubes from one side of dichotomous appressoria (two-step germination). In addition to the dichotomous appressoria, we detected a few conidia that produced hooked appressoria and extended germ tubes from the tip of the appressorium. Penetration attempts by KRCP-4N conidia on barley leaves were impeded by papillae formed at penetration sites beneath these two types of appressorium. From these results, we conclude that the "two-step germination" of E. trifoliorum KRCP-4N conidia is the result of an unsuccessful penetration attempt, causing diversity in appressorial shape.
  • Yoshihiro Takikawa; Ling Xu; Koji Kakutani; Teruo Nonomura; Takeshi Sameshima; Yoshinori Matsuda; Hideyoshi Toyoda
    MYCOSCIENCE SPRINGER TOKYO 52 (3) 198 - 203 1340-3540 2011/05 [Refereed]
     
    The emergence of germ tubes from the conidia of powdery mildew fungi is the first morphological event of the infection process, preceding appressoria formation, peg penetration and primary haustoria formation. Germination patterns of the conidia are specific in powdery mildew fungi and therefore considered useful for identification. In the present study, we examined conidial germination of the tomato powdery mildew Oidium neolycopersici KTP-01 in order to clarify whether germ tube emergence site in KTP-01 conidia is determined by the first contact of the conidia to leaves (as found for the conidia of barley powdery mildew), or alternatively is predetermined and is unrelated to contact stimulus. Highly germinative conidia of KTP-01 were collected from conidial pseudochains on conidiophores in colonies on tomato leaves using two methods involving an electrostatic spore attractor and a blower. In the electrostatic spore attraction method, the conidia were attracted to the electrified insulator probe of the spore collector-this being the first contact stimulus for the conidia. In addition, the blowing method was used as a model of natural infection; pseudochain conidia were transferred to detached leaves by air (1 m/s) from a blower. Thus, landing on the leaves was the first contact for the conidia. Furthermore, conidia were also blown onto an artificial membrane (Parafilm-coated glass slides forming a hydrophobic surface) or solidified agar plates in Petri dishes (hydrophilic surface). Eventually, almost all conidia on the probe and on tomato leaves or artificial hydrophobic and hydrophilic surfaces synchronously germinated within 6 h of incubation, indicating that the first contact of the conidia with any of the aforementioned substrata was an effective germination induction signal. Germ tube emergence sites were exclusively subterminal on the conidia. Moreover, the germ tubes emerged without any relation to the sites touched first on the conidia. Thus, the present study strongly indicates that conidia of O. neolycopersici produce germ tubes at a predetermined site.
  • Yoshinori Matsuda; Teruo Nonomura; Koji Kakutani; Yoshihiro Takikawa; Junji Kimbara; Yoshihiro Kasaishi; Kazumi Osamura; Shin-ichi Kusakari; Hideyoshi Toyoda
    CROP PROTECTION ELSEVIER SCI LTD 30 (2) 155 - 162 0261-2194 2011/02 [Refereed]
     
    A bifunctional electric field screen was proposed to physically exclude insect pests from warehouses. The screen consists of insulated iron wires (ICW) arranged in parallel and two earthed conductor nets placed on both sides of the ICW. A negative charge (0.1-8.0 kV) was applied to the insulated wires with a voltage generator to polarize an insulator sleeve used to cover the wire, negatively on the outer surface and positively on the inner conductor wire surface of the sleeve. The negative surface charge of the ICW caused an electrostatic induction in the earthed nets and an opposite charge on the net surfaces facing the ICW. An electric field formed in a space between the ICW and the earthed net, and the field strength increased in direct proportion to increasing voltages applied to the ICW. Adults of the test insects (cigarette beetle (Lasioderma serricorne) and vinegar fly (Drosophila melanogaster)) reaching the outer surface of the earthed net were deterred from entering the inside of the charged screen, whereas all insects immediately passed through the screen when the ICW was not charged. This avoidance was directly proportional to the increase in the voltage. In addition, the capability of the screen to capture insects that enter inside the screen was proven by introducing insects into the space between the ICW and the earthed net. Strong capture was observed when the ICW was negatively charged with more than 4.1 kV, under which conditions a short-term electric current (peaking at 0.3-0.6 mu A, for 3 min) occurred transiently. This electric current was due to the release of electricity from the insects, giving a net overall positive charge to the insects, which therefore were attracted more strongly to the negatively charged ICW. A test using an attractant-set chamber showed that the insects were completely prevented from passing through the charged screen, in contrast to a rapid transfer of all insects when the screen was not charged. Thus, the present results show that the described screen is a promising physical tool for controlling insect pests in warehouses. (C) 2010 Elsevier Ltd. All rights reserved.
  • 瀧川 義浩; 角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉
    Memoirs of Institute of Advanced Technology, Kinki University 近畿大学先端技術総合研究所 16 (16) 7 - 18 1346-8693 2011 
    [要旨] トマトは、栽培種トマトと野生種トマトに大きく分類されている。栽培種トマトは我が国の主要作物であり、その用途は生鮮や加工品など多岐にわたる。また、食品機能性に富み、これまでに、抗酸化作用、抗炎症作用、抗ガン作用、抗アレルギー作用など、様々な機能性が認められている。一方、野生種トマトに関しては、既に著者らのグループにおいて、昆虫に対する忌避作用や抗菌作用などの効果を明らかにしており、さらに多くの機能性物質の存在が示唆されている。そこで本研究では、各種野生種トマトの栽培試験により優良形質のトマトを選抜し、それらの機能性成分の探索、ならびにアンチエイジング素材の開発を目的とした、生物寿命に及ぼす影響についての検討を行った。[Abstract] In order to examine nutraceutical effects of wild tomato species, we screened wild tomato species that were suitable for cultivation and extracted substances useful for longevity prolongment. Namely, twentyone of totally of 41wild tomato species used possibly produced fruits of different shapes, colors（red, green and purple）and sizes. Antioxidative and polyphenolic substances were detected in some fractions of fruit- and leaf-extracts obtained from both common and wild tomato species. All fractions of leafextracts were less effective to prolong life span of the test insect, with no relation to common and wild tomato plants. In point A, there was no significant difference in the longevity prolongment of the test insect among all fractions of both common and wild tomato plants. In point B, two fractions （hexanesoluble and ethyl acetate-soluble fractions）of fruit-extracts from both common and wild tomato plants were positive to the lifespan prolongment of vinegar fly adults. Hexane-soluble fraction of fruit-extracts from wild tomato plants was most effective to prolong lifespan of the test insect. In point C, ethyl acetatesoluble fraction of fruit-extracts from both plants showed the significant effect on the prolongment of lifespan. Antioxidants and polyphenolic substances in ethyl acetate-soluble fractions were effective to prolong lifespan of the test insect. Polyphenols are potential substances to extend lifespan of diverse species including vinegar fly. Hexane-soluble fraction of fruit-extracts from wild tomato plants was positive to the lifespan prolongment, it contained substances effective to prolong lifespan of the test insect. Judging from these results, both common and wild tomatoes are useful sources to search various nutraceutical substances for the longevity prolongment, as assessed by the insect assay system.
  • Teruo Nonomura; Ayae Nishitomi; Yoshinori Matsuda; Chiyomi Soma; Ling Xu; Koji Kakutani; Yoshihiro Takikawa; Hideyoshi Toyoda
    FUNGAL BIOLOGY ELSEVIER SCI LTD 114 (11-12) 917 - 928 1878-6146 2010/11 [Refereed]
     
    The appressorial shapes of the powdery mildews are an important clue to the taxonomy of the powdery mildew fungi, but the conidia of the tomato powdery mildew Oidium neolycopersici KTP-01 develop non-lobed, nipple-shaped, and moderately lobed or multilobed appressoria on the same leaves. To remove this ambiguity, we performed consecutive observations of sequential appressorial development of KTP-01 conidia with a high-fidelity digital microscope. Highly germinative conidia of KTP-01, collected from conidial pseudo-chains formed on the tomato leaves, were inoculated into host tomato and nonhost barley leaves or an artificial hydrophobic membrane (Parafilm). Events from germination initiation to appressorium formation were synchronous in all conidia on all materials used for inoculation, but post-appressorial behaviors varied among the materials. Appressoria on the membrane-stuck glass slide formed several projections at different portions of the appressoria to repeat unsuccessful penetration attempts. Similar unsuccessful penetration behavior by KTP-01 conidia was observed in the inoculations into leaves of barley plants, wild tomato species Lycopersicon peruvianum LA2172 (carrying the Ol-4 gene for powdery mildew resistance), and a susceptible host tomato (Lycopersicon esculentum) that had been inoculated with the barley powdery mildew (Blumeria graminis f. sp. hordei, race 1) conidia. On the barley leaves, all penetrations of KTP-01 were impeded by the papillae formed beneath the sites of the appressorial projections. On both the wild tomato and the race 1-inoculated cultivated tomato plants, KTP-01 conidia were prevented from forming functional haustoria by hypersensitive epidermal cell death; this hypersensitive reaction involved the Ol-4 gene in the wild tomato plants or the 'induced resistance' acquired by the nonpathogenic conidia previously inoculated into the cultivated tomato plants. All these KTP-01 conidia produced several projections on the appressoria during the repeated unsuccessful penetration attempts and eventually exhibited multilobed appressoria. On the host tomato leaves inoculated singly with KTP-01 conidia, fewer than 20 % of the conidia located appressoria on the central part of target epidermal cells and succeeded in forming functional haustoria at the first penetration attempt without forming an appressorial projection. These conidia exhibited non-lobed appressoria. The remaining conidia, however, whose appressoria were located on/near the border of the target epidermal cells, were more likely to fail to penetrate at the first penetration, and then to develop additional projections for subsequent penetrations. Most conidia succeeded in forming functional haustoria at the second to fourth penetration attempts, but a few conidia failed to produce haustoria at all attempted penetrations. Eventually, the conidia that succeeded at the second penetration possessed a single appressorial projection (exhibiting the nipple-shaped appressoria), whereas the remaining conidia exhibited moderately lobed appressoria with two to four appressorial projections and multilobed appressoria, with more projections. Thus, the present study revealed that the basic shape of appressoria of KTP-01 was the non-lobed type, and that polymorphic changes of the appressoria occurred as a result of successive production of projections during repeated unsuccessful penetration attempts. (C) 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
  • T. Nonomura; S. Komaki; L. Xu; N. Moriura; H. Ioroi; S. Takashima; K. Kakutani; Y. Takikawa; Y. Matsuda; H. Toyoda
    PLANT BREEDING WILEY-BLACKWELL PUBLISHING, INC 128 (3) 282 - 289 0179-9541 2009/06 [Refereed]
     
    The feedback-insensitive anthranilate synthase (AS) gene was used as a selection marker for transformants of Arabidopsis thaliana. The mutant gene (mAS1-2) was constructed by substituting nucleotide at the effector-binding site of the intrinsic AS gene via PCR-mediated site-directed mutagenesis and flanked with the myrosinase promoter pyk10 to drive its expression during initial root elongation. This inducible gene cassette was first introduced into Agrobacterium tumefaciens and then delivered into A. thaliana by floral-dip inoculation. 5-methyltryptophan (5-MT) inhibited AS and suppressed seedling growth of wild type plants as a result of tryptophan starvation. With the addition of sucrose (10 mg/ml), 5-MT inhibited cotyledon opening and caused anthocyanin to accumulate in juvenile seedlings. The present mutant reversed the tryptophan starvation caused by 5-MT and blocked subsequent sugar responses. The sugar responses were detected in non-transformed plants grown on a selection medium containing 10 mg/ml of sucrose and 10 mu g/ml of 5-MT after 3 days of incubation. Thus, true transformants could be selected after a short incubation, compared to the conventional kanamycin-selection method that did not eliminate all non-transformed plants.
  • Teruo Nonomura; Yoshinori Matsuda; Ling Xu; Koji Kakutani; Yoshihiro Takikawa; Hideyoshi Toyoda
    MYCOLOGICAL RESEARCH ELSEVIER SCI LTD 113 (3) 364 - 372 0953-7562 2009/03 [Refereed]
     
    A population of simultaneously germinating conidia is an ideal inoculum of the powdery mildew pathogen, Oidium neolycopersici. In conditions of no or low wind velocity, O. neolycopersici successively stacks mature conidia on conidiophores in a chain formation (pseudochain), without releasing the precedent mature conidia. These pseudochain conidia represent a perfect inoculum, in which all conidia used for inoculation germinate simultaneously. However, we found that conidia must be collected before they fall to the leaf surface, because the germination rate was lower among conidia deposited on the leaf surface. We used an electrostatic spore collector to collect the pseudochain conidia, and their high germination rate was not affected by this treatment. The spore collector consisted of an electrified insulator probe, which created an electrostatic field around its pointed tip, and attracted conidia within its electric field. The attractive force created by the probe tip was directly proportional to voltage, and was inversely proportional to the distance between the tip and a target colony on a leaf. Pseudochain conidia were successfully collected by bringing the electrified probe tip close to target colonies on leaves. In this way, conidia were collected from colonies at 3-d intervals. This effectively collected all conidia from conidiophores before they dropped to the leaf surface. A high germination rate was observed among conidia attracted to the probe tip (95.5 +/- 0.6 %). Conidia were easily suspended in water with added surfactant, and retained their germination ability. These conidia were infective and produced conidia in pseudochains on conidiophores after inoculation. The electrostatic spore collection method can be used to collect conidia as they form on conidiophores, thus obtaining an inoculum population in which all of the conidia germinate simultaneously. (c) 2008 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
  • 温室内で発生したトマト根腐萎凋病（Fusarium crown and root rot disease）の感染拡大に関わるミギワバエScatella stagnalis（Diptera: Ephydridae）の影響.
    松田克礼; 角谷晃司; 野々村照雄; 瀧川義浩
    近畿大学農学部紀要 近畿大学農学部 42 237 - 242 0453-8889 2009/03
  • Teruo Nonomura; Ling Xu; Mizuki Wada; Shuzou Kawamura; Takemasa Miyajima; Ayae Nishitomi; Koji Kakutani; Yoshihiro Takikawa; Yoshinori Matsuda; Hideyoshi Toyoda
    PLANT SCIENCE ELSEVIER IRELAND LTD 176 (1) 31 - 37 0168-9452 2009/01 [Refereed]
     
    Secretion by glandular trichomes is thought to be a strategy to retain moisture water on leaves under drought conditions. In the wild tomato species Lycopersicon pennellii exudates accumulate at the apical terminal of the trichome, forming a bulbous head. During a 30 s mist treatment, the exudates condensed moisture water to form water drops on the trichome heads, and water drops were absorbed through trichomes by the plants. However, a longer mist treatment (40 s) caused the drops on all trichome heads to drop down to the leaf surface. Water drops containing the exudates were amphiphilic and spread over the leaf surface in a thin layer. This is an additional mechanism of the glandular trichomes for covering leaves with the exudates as a chemical boundary layer. Exudates of type IV glandular trichomes of L. pennellii showed antifungal activity against conidia of the tomato powdery mildew pathogen Oidium neolycopersici. The exudates covering the leaves completely suppressed germination of inoculated conidia, which prevented subsequent infection events such as formation of appressoria and haustoria. On non-misted leaves almost all conidia germinated and formed appressoria, resulting in successful infection. L. pennellii is susceptible to tomato powdery mildew, but the mechanisms for condensing mist water by type IV trichome-derived exudates can contribute to suppressing foliar infection by the powdery mildew pathogen. Crown Copyright (C) 2008 Published by Elsevier Ireland Ltd. All rights reserved.
  • T. Nonomura; Y. Matsuda; K. Kakutani; Y. Takikawa; H. Toyoda
    CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE CANADIAN PHYTOPATHOLOGICAL SOC 30 (4) 517 - 524 0706-0661 2008/10 [Refereed]
     
    We devised a cylindrical electrostatic discharge generator to physically eradicate tomato powdery mildew colonizing tomato leaves. The generator consists of a copper needle with a pointed tip, an insulating acrylic cylinder, and an electrostatic voltage generator. The needle is insulated with a vinyl sleeve, except for the pointed tip, and is coaxially fixed in the cylinder and connected to the voltage generator. The needle is negatively charged, and the treated plant is earthed. In initial tests, a corona, characterized by a blue glow, formed at the needle tip as the probe was brought closer to the leaf surface. The distance at which this Occurred increased from 16 to 50 mm as the voltage was increased from 5 to 30 kV. If the probe was brought too close to the leaf surface, an arc discharge Occurred that caused injury to the leaf. Powdery mildew colonies were destroyed by 2-second exposures at probe distances intermediate to where corona discharge was initiated and where arcing Occurred. A probe distance of 25 mm and 30 kV for a 2-second burst was selected to further test the efficacy of the probe for controlling powdery mildew in a greenhouse environment. Tomato plants were grown hydroponically in two open-window greenhouses under a first-truss cropping system. Colonies appeared on tomato leaves 10 to 14 days after transplanting. During the following 2 weeks, these colonies produced abundant progeny conidia that secondarily infected neighboring plants. Corona discharge treatment in one greenhouse, at the stage when colonies first became visible, completely Suppressed the spread of the disease compared with a non-treated greenhouse in which disease spread rapidly. The present discharge generator is portable and easy to operate on-site as a part of routine care of hydroponically Cultured tomatoes in greenhouses and provides a non-chemical method to control powdery mildew disease.
  • Makio Shibano; Koji Kakutani; Masahiko Taniguchi; Masahide Yasuda; Kimiye Baba
    JOURNAL OF NATURAL MEDICINES SPRINGER TOKYO 62 (3) 349 - 353 1340-3443 2008/07 [Refereed]
     
    The dayflower, Commelina communis L., contains 1-deoxynojirimycin (DNJ) and (2R,3R,4R,5R)2,5-bis(hydroxymethyl)-3,4-dihydroxypyrrolidine (DMDP), potent alpha-glucosidase inhibitors. The extracts and powder of this herb are important food materials for prophylaxis against type 2 diabetes. Eleven flavonoid glycosides as antioxidants, isoquercitrin, isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-beta-D-glucoside, glucoluteolin, chrysoriol-7-O-beta-D-glucoside, orientin, vitexin, isoorientin, isovitexin, swertisin, and flavocommelin, were identified from the aerial parts of C. communis. Their antioxidant activities were measured using in vitro assays employing the 1,1-diphenyl-2-picrylhydrazyl radical- and superoxide radical-scavenging assays. The results showed that glucoluteolin, orientin, isoorientin, and isoquercitrin are the predominant antioxidants in this herb. Moreover, isoquercitrin, isorhamnetine-3-O-rutinoside, vitexin, and swertisin inhibited the activity of alpha-glucosidase from rat intestine.
  • Jun Murata; Hiroyuki Oki; Koji Kakutani; Takashi Hashimoto
    PLANT BIOTECHNOLOGY JAPANESE SOC PLANT CELL & MOLECULAR BIOL 25 (2) 157 - 163 1342-4580 2008 [Refereed]
     
    Angelica acutiloba and related species have been known for their analgesic and sedative effects, and are widely used as the key ingredient in Japanese traditional Kampo medicine. Among various varieties, Angelica acutiloba var. acutiloba has long been grown in the Obuka area of Nara prefecture, Japan, and has been evaluated as an excellent variety. Recent cultivation of this and other varieties of Angelica in various regions of East Asia prompted us to identify DNA polymorphisms that distinguish the acutiloba variety from others. Random Amplified Polymorphic DNA analysis and sequencing selected genomic regions among Angelica plants of different origins identified several nucleotide changes among the varieties tested, which enabled us to distinguish the acutiloba variety from others. We also report an improved protocol for genomic DNA extraction from dried root samples of Angelica.
  • MATSUDA Yoshinori
    Crop Protection 27 (2) 215 - 221 0261-2194 2008/01 [Refereed]
     
    A new electrostatic insect-proof screen (electric dipolar screen) was developed using insulated conductor wire. Copper conductor wire is encased in a flexible transparent vinyl insulator sleeve and charged with an electrostatic voltage generator. Paired insulated conductor wires were placed in parallel with 5mm of separation and oppositely charged with 15kV DC using separate electrostatic voltage generators. A negatively charged conductor wire polarizes the insulator negatively at the outer surface and positively on the conductor side, and a positively charged conductor wire polarizes vice versa. A pair of insulated conductor wires with opposite surface charges was used as an electric dipole. An electric dipole creates an electrostatic force from positive to negative poles. This force was harnessed to trap whiteflies entering greenhouses. Dipolar wires were attached in parallel to a frame to construct an electric dipolar screen that could be fitted to greenhouse windows. The screen prevented adult whiteflies from passing through spaces (up to 30mm) between the wires of the screen, and tomato plants inside remained free of whiteflies throughout the entire 3-week experiment, in contrast to heavy infestation of all plants in the uncharged area. Thus, the electric dipolar screen is a promising tool to physically exclude flying whiteflies from greenhouses.
  • MATSUDA Yoshinori
    Plant Pathology 56 (6) 987 - 997 0032-0862 2007/03 [Refereed]
  • Kiyohisa Kawamura; Tuyosi Sugimoto; Koji Kakutani; Yoshinori Matsuda; Hideyoshi Toyoda
    APPLIED ENTOMOLOGY AND ZOOLOGY JAPAN SOC APPL ENTOMOL ZOOL 42 (1) 89 - 96 0003-6862 2007/02 [Refereed]
     
    Genetic variations of the internal transcribed spacer-1 region in sweet potato weevil, Cylasfornficarius, were examined from 27 collection sites in the main infested areas in the world, except Africa, in this study. The ITS-1 lengths of 36 weevils tested in this study ranged from 557 to 587 bp, and were conspicuously longer in weevils from India. The genetic distances between weevils from India and from the other areas tested were larger than among weevils from the latter areas. The phylogenetic tree based on ITS-1 sequences consisted of two main clades of India and East Asia consisting of Northeast Asia and Southeast Asia subclades. The former subclade was also divided into three minor subclades. Weevils from Georgia and Hawaii (USA), and St. Kitts (the West Indies) belonged to the same subclade as those from Guangdong (China) and Hanoi (Vietnam). Also, weevils from the Ogasawara Islands (Japan) belonged to the same subclade. On the other hand, weevils from the Southwest Islands (Japan) belonged to the same subelade as those from Taiwan. When referring to human historical events, C. formicarius would have gradually spread in southern Asia due to its limited dispersal ability since originating in the Indian subcontinent 90 million years ago. However, after becoming associated with sweet potato, especially after the nineteenth century, local populations with fairly different genetic properties from Indian weevils would have been rapidly spread by human transportation of infested sweet potato throughout the world.
  • Yoshinori Matsuda; Hiroki Ikeda; Nobuyuki Moriura; Norio Tanaka; Kunihiko Shimizu; Wataru Oichi; Teruo Nonomura; Koji Kakutani; Shin-ichi Kusakari; Katsuhide Higashi; Hideyoshi Toyoda
    PHYTOPATHOLOGY AMER PHYTOPATHOLOGICAL SOC 96 (9) 967 - 974 0031-949X 2006/09 [Refereed]
     
    In an attempt to physically protect greenhouse tomato plants from the powdery mildew fungus Oidium neolycopersici, we developed a new electrostatic spore precipitator in which a copper wire conductor is linked to an electrostatic generator and covered with a transparent acrylic cylinder (insulator). The conductor was negatively charged by the generator, and the electrostatic field created by the conductor was used to dielectrically polarize the insulator cylinder. The dielectrically polarized cylinder also produced an electrostatic force without a spark discharge. This force was directly proportional to the potential applied to the conductor and was used to attract conidia of the pathogen. The efficacy of this spore precipitator in protecting hydroponically cultured tomato plants from powdery mildew was evaluated in the greenhouse. The hydroponic culture troughs were covered with a cubic frame installed with the spore precipitator, and the disease progress on precipitator-guarded and unguarded seedlings was traced after the conidia were disseminated mechanically from inoculum on tomato plants. Seedlings in the guarded troughs remained uninfected during the entire experiment, in spite of rapid spread of the disease to all leaves of the unguarded seedlings.
  • N. Moriura; Y. Matsuda; W. Oichi; S. Nakashima; T. Hirai; T. Nonomura; K. Kakutani; S. Kusakari; K. Higashi; H. Toyoda
    PLANT PATHOLOGY WILEY-BLACKWELL 55 (3) 367 - 374 0032-0862 2006/06 [Refereed]
     
    Conidia from living conidiophores of barley powdery mildew (Blumeria graminis f.sp. hordei) on host leaves were collected consecutively using an electrostatic spore collector. The collector consisted of an electrical conductor plate linked to an electrostatic voltage generator and insulator plates placed abreast on a timed conveyer. The conductor plate was negatively charged by the potential supplied from the voltage generator. The negatively charged conductor plate caused dielectric polarization of the insulator plate, and the surface charge on the insulator plate attracted mature conidia abstricted from conidiophores on colonies growing on leaves placed 2 cm from the insulator plate. The surface charge on the insulator plate was proportional to the voltage applied to the conductor plate. Under optimized conditions, abstricted conidia were attracted to the electrostatically activated insulator plates without any detriment to their survival. During a colony's life span of c. 460 h, conidia were released throughout the day and c. 12 x 10(4) conidia were collected during the lifetime of the colony. This is the first report on the direct quantification of progeny conidia produced by powdery mildew infecting host leaves.
  • N Moriura; Y Matsuda; W Oichi; S Nakashima; T Hirai; T Sameshima; T Nonomura; K Kakutani; S Kusakari; K Higashi; H Toyoda
    MYCOLOGICAL RESEARCH CAMBRIDGE UNIV PRESS 110 (1) 18 - 27 0953-7562 2006/01 [Refereed]
     
    Conidial formation and secession by living conidiophores of Blumeria graminis f. sp. hordei on barley leaves were consecutively monitored using a high-fidelity digital microscopic technique combined with electrostatic micromanipulation to trap the released conidia. Conidial chains formed on conidiophores through a series of septum-mediated division and growth of generative cells. Apical conidial cells on the conidiophores were abstricted after the conidial chains developed ten conidial cells. The conidia were electrically conductive, and a positive charge was induced in the cells by a negatively polarized insulator probe (ebonite). The electrostatic force between the conidia and the insulator was used to attract the abstricted conidia. from the conidiophores on leaves. This conidium movement from the targeted conidiophore to the rod was directly viewed under the digital microscope, and the length of the interval between conidial septation and secession, the total number of the conidia produced by a single conidiophore, and the modes of conidiogenesis were clarified. During the stage of conidial secession, the generative cells pushed new conidial cells upwards by repeated division and growth. The successive release of two apical conidia was synchronized with the successive septation and growth of a generative cell. The release ceased after 4-5 conidia were released without division and growth of the generative cell. Thus, the life of an individual conidiophore (from the erection of the conidiophore to the release of the final conidium) was shown to be 107 h and to produce an average of 33 conidia. To our knowledge, this is the first report on the direct estimation of life-long conidial production by a powdery mildew on host leaves. (c) 2005 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
  • Y Matsuda; T Sameshima; N Moriura; K Inoue; T Nonomura; K Kakutani; H Nishimura; S Kusakari; S Takamatsu; H Toyoda
    PHYTOPATHOLOGY AMER PHYTOPATHOLOGICAL SOC 95 (10) 1137 - 1143 0031-949X 2005/10 [Refereed]
     
    Greenhouse-grown tomato seedlings were inoculated naturally with two genera of powdery mildew conidia forming appressorial germ tubes that could not be differentiated by length alone. For direct identification, single germinated conidia were removed from leaves by means of a glass pipette linked to the manipulator of a high-fidelity digital microscope. This microscope enabled in vivo observation of the fungi without leaf decoloration or fungal staining. The isolated conidia were subjected to PCR amplification of the 5.8S rDNA and its adjacent internal transcribed spacer sequences followed by nested PCR to attain sensitivity high enough to amplify target nucleotide sequences (PCR/nested PCR). Target sequences from the conidia were completely coincident with those of the pathogen Oidium neolycopersici or Erysiphe trifolii (syn. Microsphaera trijblii), which is nonpathogenic on tomato. Using RT-PCR/nested PCR or multiplex RT-PCR/nested PCR, it was possible to amplify transcripts expressed in single conidia. Conidia at pre- and postgermination stages were removed individually from tomato leaves, and two powdery mildew genes were monitored. The results indicated that the P-tubulin homolog TUB2-ol was expressed at pre- and postgermination stages and the cutinase homolog CUT1-ol was only expressed postgermination. Combining digital microscopic micromanipulation and two-step PCR amplification is thus useful for investigation of individual propagules on the surface of plants.
  • 豊田 秀吉; 松田 克礼; 角谷 晃司
    Mem. Fac. Agr. Kinki Univ Kinki University 36 9 - 18 0453-8889 2005/03 
    本実験では、顕微鏡下で標的とした単一細胞からマイクロピペットを用いて細胞内容物を吸引し、そこに存在するmRNAを鋳型とした単一細胞RT-PCR法を適用することとした。病原菌の感染によって発現が誘導される遺伝子（CHI2、GLUなど）を検索した。誘導型遺伝子の発現を厳密に評価するため、RT-PCRを行う際に、先の構成的発現遺伝子を増幅するプライマーを混合し、指標遺伝子として同時に増幅することにより、誘導型遺伝子の発現検出を行った。その結果、病原菌の感染を受けていない細胞およびその感染を受けている両者の細胞において指標とした構成的発現遺伝子は検出された。誘導型遺伝子として使用したCHI2遺伝子およびGLU遺伝子は病原菌の感染を受けていない細胞においてその発現が検出され、うどんこ病菌の感染過程において抑制される傾向にあった。
  • K Fujita; Y Matsuda; M Wada; Y Hirai; K Mori; N Moriura; T Nonomura; K Kakutani; H Toyoda
    PLANT CELL REPORTS SPRINGER 23 (7) 504 - 511 0721-7714 2004/12 [Refereed]
     
    Two-step PCR (RT-PCR and nested PCR) was used to detect gene expression in powdery mildew pathogen-infected cells of detached inner epidermis of barley coleoptiles. Cellular contents of infected cells were microscopically suctioned with a micropipette and subjected to PCR. Triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase genes involved in the glycolytic pathway and a stimulus-induced endochitinase gene were targeted, and their expression was determined by detecting cDNAs derived from spliced transcripts. The two gycolysis-related genes were constantly expressed in the tissue irrespective of pathogen inoculation. In contrast, chitinase gene expression was induced in non-infected inner epidermis after detachment. After inoculation, this expression was selectively suppressed in pathogen-invaded cells, in spite of continuous expression in non-invaded cells of the same epidermis. Thus, the present method enabled us to directly analyze transcripts in individual cells at the infection site and assess the capability of the pathogen to regulate host gene expression.
  • Wataru Oichi; Yoshinori Matsuda; Takeshi Sameshima; Teruo Nonomura; Koji Kakutani; Hiroaki Nishimura; Shin-Ichi Kusakari; Hideyoshi Toyoda
    Journal of General Plant Pathology 70 (6) 329 - 332 1345-2630 2004/12 [Refereed]
     
    Conidiogenesis by Oidium neolycopersici KTP-01 on tomato leaves was vitally monitored with a high-fidelity digital microscope. Conidiophores were initially formed 3 days after inoculation and then elongated to a maximum length within at least 12 h. The apical part was split into two cells after two successive septations, accompanied by apical expansion. These cells subsequently developed into primary and secondary conidia. An additional septation at the stem portion of the conidiophores produced a generative and a foot cell. Subsequent conidiation occurred during repeated cycles of splitting of the generative cell, maturation of the apical cell into a conidium, and abstriction of the conidium. To our knowledge, this report is the first on the developmental process of conidiogenesis by powdery mildew on host leaves as revealed with the digital microscope.
  • M Wada; Y Matsuda; K Fujita; A Nanjo; M Nishimura; T Nonomura; K Kakutani; H Toyoda
    PLANT CELL TISSUE AND ORGAN CULTURE KLUWER ACADEMIC PUBL 79 (1) 109 - 114 0167-6857 2004/10 [Refereed]
     
    RT-PCR was used to detect gene expression in situ in single selected cells of tomato callus aggregates. The cytoplasm from one cell was removed with a micropipette viewed under a light microscope and used directly for RT-PCR, followed by nested PCR. This method to remove cytosolic contents prevented the introduction of genomic DNA into the RT-PCR, and only intron-spliced products were amplified when intron-containing genes were used as PCR targets. In addition, transcription of the intron-free gene was possibly detected by simultaneously tracing the intron-containing and intron-free genes using mixed primers for the targeted genes. The present study indicated that some stimuli-activated genes, such as CHI3 and TLC1-LTR, were constitutively transcribed in tomato callus cells.
  • Takeshi Sameshima; Koichi Kashimoto; Keiko Kida; Yoshinori Matsuda; Teruo Nonomura; Koji Kakutani; Kengo Nakata; Shin-Ichi Kusakari; Hideyoshi Toyoda
    Journal of General Plant Pathology 70 (1) 7 - 10 1345-2630 2004/02 [Refereed]
     
    Leaves of tomato and barley were inoculated with conidia of Blumeria graminis f. sp. hordei race 1 (R1) or Oidium neolycopersici (KTP-01) to observe cytological responses in search of resistance to powdery mildew. Both conidia formed appressoria at similar rates on tomato or barley leaves, indicating that no resistance was expressed during the prepenetration stage of these fungi. On R1-inoculated tomato leaves, appressoria penetrated the papillae, but subsequent haustorium formation was inhibited by hypersensitive necrosis in the invaded epidermal cells. On the other hand, KTP-01 (pathogenic to tomato leaves) successfully developed functional haustoria in epidermal cells to elongate secondary hyphae, although the hyphal elongation from some conidia was later suppressed by delayed hypersensitive necrosis in some haustorium-harboring epidermal cells. Thus, the present study indicated that the resistance of tomato to powdery mildew fungi was associated with a hypersensitive response in invaded epidermal cells but not the prevention of fungal penetration through host papilla.
  • トマトうどんこ病ー動的解析法とその有効防除の確立をめざして 植物病の探究 In Pursuit of the Essence of Plant Pathogenesis
    角谷 晃司; 豊田 秀吉; 松田 克礼; 野々村 照雄
    「植物病の探究」出版会 2004
  • 異なる支持体を用いたカンゾウの水耕栽培によるグリチルリチン生産
    角谷 晃司
    薬学総合研究所紀要 近畿大学薬学総合研究所 12 133 - 138 2003/03
  • Analysis of UDP.glucuronosyltrans ferase gene of Glycyrrhiza glabra L.
    角谷 晃司
    薬学総合研究所紀要 近畿大学薬学総合研究所 11 105 - 110 2003/03 
    ディデネレートプライマーを利用したRT.PCR法により、甘草根のtotal RNAから糖転移酵素遺伝子を増幅した。この遺伝子はエンドウのグルクルタン酸転移酵素遺伝子と高い相同性が示され、甘草のグリチルチリン生合成に関与する糖転移酵素遺伝子であると示唆された。
  • Direct RT-PCR amplification of mature mRNAs in single trichome cells of plant leaves
    角谷 晃司; 松田 克礼; 野々村 照雄; 豊田 秀吉; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科
    Recent Res. Devel. Cell Biol Recent Res. Devel. Cell Biol 2003 [Refereed]
  • 角谷 晃司; 松田 克礼; 野々村 照雄; 豊田 秀吉; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科
    J. Gen. Plant Pathol 69 (2) 131 - 137 1345-2630 2003 [Refereed]
  • 角谷 晃司; 松田 克礼; 野々村 照雄; 豊田 秀吉; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科; 大阪府環境農林水産総合研究所
    J. Gen. Plant Pathol 69 (6) 406 - 408 1345-2630 2003 [Refereed]
  • Y Iida; Y Matsuda; R Saito; M Nakasato; T Nonomura; K Kakutani; Y Tosa; S Mayama; H Toyoda
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY TAYLOR & FRANCIS LTD 67 (1) 198 - 202 0916-8451 2003/01 [Refereed]
     
    Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with beta-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes.
  • Takeshi Fukumoto; Teruo Nonomura; Yoshinori Matsuda; Shin-ichirou Komaki; Nobuyuki Moriurai; Hideyoshi Toyoda; Koji Kakutani; Akiyoshi Sawabe
    Plant Biotechnology 20 (3) 257 - 261 1347-6114 2003 [Refereed]
     
    When leaf segments of a tomato cultivar 'Ponderosa' were inoculated with Agrobacterium rhizogenes MAFF07-20001 carrying the binary vectors pRi and pBI121/sGFP, adventitious roots were developed from calli formed at the edges of the segments. Primordial roots were obtained with green fluorescence under blue light and elongated vigorously on hormone-free medium without loss of the green fluorescence. They were easily distinguishable from the non-fluorescing roots on the same segments. Successful integration of the sGFP and rol C genes into the chromosome of tomato roots was confirmed by polymerase chain reaction and Southern hybridization. The present method enables us to evaluate the hairy root formation without subculture, isolation and DNA analysis. All commercial cultivars available in Japan (24 cultivars) and 14 breeding lines of tomato were tested by this method. All but two breeding lines produced the hairy roots. Thus, the present method is useful for hairy root production in tomato. © 2003, Japanese Society for Plant Cell and Molecular Biology. All rights reserved.
  • 角谷 晃司; 松田 克礼; 野々村 照雄; 豊田 秀吉; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科; 大阪府立農林技術センター; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科
    Journal of General Plant Pathology 69 (3) 176 - 185 1345-2630 2003 [Refereed]
     
    近畿大学構内で発生した新しいトマトうどんこ病菌について、その特性を調査し、Oidium neolycopersiciであると同定した。また、本病原菌の他の植物に対する感染性を調べ、その宿主範囲についても明らかにした。
  • 豊田 秀吉; 石井雄規; 藤原圭一; 松田 克礼; 野々村 照雄; 瀧川義治; 角谷 晃司; 土佐幸雄; 眞山
    Journal of General Plant Pathology 69 (2) 131 - 137 1345-2630 2003 [Refereed]
     
    土壌から分離した植物根高度定着性細菌Enterobacter cloacaeにキトサン分解酵素遺伝子を導入し、本細菌に特異的に感染するファージを併用することによりキトサナーゼを利用したモニタリングシステムを確立した。
  • Y Iida; Y Matsuda; R Saito; M Nakasato; T Nonomura; K Kakutani; Y Tosa; S Mayama; H Toyoda
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY TAYLOR & FRANCIS LTD 67 (1) 198 - 202 0916-8451 2003/01 [Refereed]
     
    Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with beta-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes.
  • 甘草のバイオテクノロジー
    角谷 晃司
    KAMPO EYES KAMPO EYES 近畿大学東洋医学研究所 3 4  2002/09 
    カンゾウ培養苗を用いた人工栽培によるグリチルリチン生産と、RAPD分析法によるカンゾウ種の簡易識別法について解説した。
  • アグロバクテリウム法を用いたシナカンゾウ （Glycyrrhiza echinata） の効率的な形質転換
    角谷 晃司; 尾崎和男; 渡
    薬学合研究所紀要 近畿大学薬学合研究所 10 (10) 47 - 53 2002/03 
    アグロバクテリウム法を用いて薬用植物である甘草へ遺伝子導入を試みた。 効率的に形質転換体植物が作出できたことより、 2 次代謝産物の生合成を遺伝子発現レベルで制御する代謝工学に応用できうると考えられた。
  • 豊田 秀吉; 池田千亜紀; 野々村 照雄; 松田 克礼; 角谷 晃司
    生物環境調節 日本生物環境調節学会 40 (1) 99 - 105 0582-4087 2002/03 [Refereed]
     
    トマト葉原基へ変異誘起剤処理を施し、 生長した葉において発現する変異を確認した後、 その変異葉からのカルス誘導ならびに個体再生を行なった。 その結果、 得られた再分化個体に同様の変異が認められたことから、 本法は変異育種に有効な手法であると考えた。
  • Y Takikawa; H Mori; Y Otsu; Y Matsuda; T Nonomura; K Kakutani; Y Tosa; S Mayama; H Toyoda
    JOURNAL OF APPLIED MICROBIOLOGY BLACKWELL PUBLISHING LTD 93 (6) 1042 - 1050 1364-5072 2002 [Refereed]
     
    Aims: To establish a rapid and efficient method for detecting Enterobacter cloacae based on chitinase gene transformation and lytic infection by virulent bacteriophages. Methods and Results: A phylloplane strain of E. cloacae was isolated from tomato leaves and transformed with a chitinase gene. Transformed bacteria were collected from single colonies and infected with newly isolated, virulent bacteriophages in the presence of the chitinase substrate 4-methylumbelliferon (4MU)-(GlcNac)(3). To assay chitinase activity in the lysates, the product 4MU was measured spectrofluorophotometrically or visibly detected under u.v. irradiation. Chitinase gene-transformed bacteria obtained from single colonies could be specifically identified in 30 min by the emission of 4MU fluorescence following lysis caused by phage infection. Conclusions: The chitinase gene was used as a reporter gene to construct a new system for easy and rapid monitoring of transgenic strains of E. cloacae released in the environment, in combination with specific recognition by virulent bacteriophages. Significance and Impact of the Study: The assay is simple, rapid, inexpensive, easy to perform and applicable to other strains. The system can be used for the routine monitoring of bacteria, which is important because of the increased use of transgenic strains of E. cloacae as an antagonistic biological control agent for plant diseases.
  • Y Takikawa; H Mori; Y Otsu; Y Matsuda; T Nonomura; K Kakutani; Y Tosa; S Mayama; H Toyoda
    JOURNAL OF APPLIED MICROBIOLOGY BLACKWELL PUBLISHING LTD 93 (6) 1042 - 1050 1364-5072 2002 [Refereed]
     
    Aims: To establish a rapid and efficient method for detecting Enterobacter cloacae based on chitinase gene transformation and lytic infection by virulent bacteriophages. Methods and Results: A phylloplane strain of E. cloacae was isolated from tomato leaves and transformed with a chitinase gene. Transformed bacteria were collected from single colonies and infected with newly isolated, virulent bacteriophages in the presence of the chitinase substrate 4-methylumbelliferon (4MU)-(GlcNac)(3). To assay chitinase activity in the lysates, the product 4MU was measured spectrofluorophotometrically or visibly detected under u.v. irradiation. Chitinase gene-transformed bacteria obtained from single colonies could be specifically identified in 30 min by the emission of 4MU fluorescence following lysis caused by phage infection. Conclusions: The chitinase gene was used as a reporter gene to construct a new system for easy and rapid monitoring of transgenic strains of E. cloacae released in the environment, in combination with specific recognition by virulent bacteriophages. Significance and Impact of the Study: The assay is simple, rapid, inexpensive, easy to perform and applicable to other strains. The system can be used for the routine monitoring of bacteria, which is important because of the increased use of transgenic strains of E. cloacae as an antagonistic biological control agent for plant diseases.
  • アグロバクテリウム法を用いたシナカンゾウ(Glycyrrhiza echinata)の効率的な形質転換
    角谷 晃司; 近畿大学薬学総合研究所; 竹田薬品工; 竹田薬品工
    薬学総合研究所紀要 10 47 - 53 2002
  • 豊田 秀吉; 松田 克礼; 飯田祐一郎; 篠木武; 野々村 照雄; 角谷 晃司
    Journal of General Plant Pathology The Phytopathological Society of Japan 67 (4) 318 - 324 1345-2630 2001/12 [Refereed]
     
    土壌から分離したキトサン分解性細菌の粗酵素画分をフザリウム菌に処理したところ、 抑制効果が認められた。 そこで、 本菌の生産するキトサナーゼを精製し、 アミノ酸配列を決定すると同時に遺伝子のクローニングを行なった。
  • 角谷 晃司; 友田勝巳; 尾崎和男; 芝野真喜雄; 草野源次
    Natural Medicines 日本生薬学会 55 (6) 287 - 293 1340-3443 2001 [Refereed]
     
    RAPD 分析により 5 種のカンゾウ属植物の分類を行うことができた。 さらに、 G. glabra と G. uralensis において特異的な PCR 増幅断片が得られ、 その塩基配列をもとに作製 RAPD マーカーは、 両者を効率的に識別することができた。
  • Classification of Glycyrrhiza Plants by Random Amplified Polymorphic DNA analysis Discrimination of Glycyrrhiza glabra L. species and G. uralensis F. species
    角谷 晃司; 友田勝巳
    薬学総合研究所紀要 薬学総合研究所 (9) 95 - 102 2001/01 
    RAPD 分析により 5 種の異なる甘草を分類することが可能になった｡ さらに､ 本分析法により得られた種特異的断片の塩基配列より､ これまで識別が困難であった G. glabra 種と G. uralensis 種を区別できる RAPD マーカーを作製することができた｡
  • Kazuhiko Matsuda; Hideyoshi Toyoda; Koji Kakutani; Masayuki Hamada; Seiji Ouchi
    Agricultural and Biological Chemistry 54 (11) 3039 - 3040 0002-1369 1990 [Refereed]

Books etc

  • 森川, 敏生 シーエムシー出版 2020/10 9784781315218 vii, 353p
  • An illustrated Manual of Electric Field Screens-Their Structures and Functions
    Koji Kakutani (Joint work)静電場スクリーン研究会 2019/03
  • 図解 静電場スクリーン その構造と機能
    角谷 晃司 (Joint work)静電場スクリーン研究会 2019/01
  • 吉川, 雅之; 村岡, 修 シーエムシー出版 2018/11 9784781313009 viii, 287p
  • 大地からの贈り物 サラシア
    角谷 晃司 (Contributor種の分類)サラシア属植物普及協会 2018/02
  • 目で見る静電場スクリーンの構造と機能
    豊田秀吉; 松田克礼; 野々村照雄; 角谷晃司 (Joint editor)静電場スクリーン研究会 2017
  • Principles and applications of Electric Field Screens
    Toyoda H; Y. Matsuda; Kusakari S; Nonomura T; Kakutani K; Takikawa Y Research Association of Electric Field Screens 2015
  • 植物工場生産システムと流通技術の最前線
    Xu L; Zhu P (Joint work植物工場における薬用植物の栽培・生産技術)エヌ・ティー・エス 2013/04
  • 吉川, 雅之; 村岡, 修 シーエムシー出版 2007/02 9784882316718 2冊
  • 新農業環境工学
    角谷 晃司 (Joint work植物を利用した有用物質の生産２．製薬産業などへの展開)養賢堂 2004/09
  • 植物病の探究
    角谷 晃司 (Joint workトマトうどんこ病ー動的解析法とその有効防除の確立をめざして)「植物病の探究」出版会 2004/09
  • Direct RT-PCR amplification of mature mRNAs in single trichome cells of plant leaves
    Y. Matsuda; K. Kakutani; M. Nishimura; M. Wada; T. Nonomura; H. Toyoda (Joint work)Recent Res. Devel. Cell Bio 2003

Conference Activities & Talks

  • 各種糖及び人工甘味料の摂食がショウジョウバエの 寿命に与える影響  [Not invited]
    中野 千鶴; 天野 耕政; 角谷 晃司
    日本薬学会第139年会  2019/03
  • サフラン（Crocus sativus）の人工栽培に関する研究  [Not invited]
    角谷 晃司
    日本生薬学会第65回年会（広島）  2018/09
  • 各種人工栽培法によるサフラン球根の生産  [Not invited]
    角谷晃司; 森健太郎
    第36回日本植物細胞分子生物学会（金沢）大会  2018/08
  • 植物工場における薬用植物など機能性植物の栽培・生産の展望  [Invited]
    角谷 晃司
    技術情報センター  2018/06
  • サフラン雌蕊抽出成分と2単糖類のαグルコシダーゼ阻害効果  [Not invited]
    角谷晃司; 角谷晃司; 河村夏季; 香川真由美; 中村恭子
    日本薬学会138年会  2018/03
  • トガリスズメバチタケ（Ophiocordyceps oxycephala）の菌糸体から得た分泌成分の研究につ いて  [Not invited]
    吉田真梨奈; 角谷晃司
    第67回日本薬学会近畿支部総会・大会  2017/10
  • 組換えZeaxanthin cleavage dioxygenase(ZCD)を用いたcrocetin 生産法の開発  [Not invited]
    香川真由美; 角谷晃司
    第67回日本薬学会近畿支部総会・大会  2017/10
  • 蟾酥成分がショウジョウバエの寿命に与える影響  [Not invited]
    中村恭子; 津川 舜; 清水康晴; 角谷晃司
    日本生薬学会第63回年会  2016/09  日本生薬学会第63回年会
  • Crocetin生産を目的としたZCD遺伝子の活用  [Not invited]
    香川真由美; 古川明典; 市川智康; 阿野友里; 岡部真依; 森井由梨; 角谷晃司
    日本生薬学会第63回年会  2016/09  富山  日本生薬学会第63回年会
  • トガリスズメバチタケ(Ophiocordyceps oxycephala)が生産するトリプシン様プロテアーゼの諸性質について  [Not invited]
    吉田真梨奈; 角谷晃司
    日本生薬学会第63回年会  2016/09  富山  日本生薬学会第63回年会
  • ゼアキサンチン高含量素材を用いた組換えZCDによるcrocetin産生  [Not invited]
    市川智康; 古川明典; 阿野友里; 香川真由美; 森井由梨; 角谷晃司
    第34回日本植物細胞分子生物学会大会  2016/09  長野  第34回日本植物細胞分子生物学会大会
  • Insect-mediated model system for screening antiaging substances: Prolonged life span of vinegar fly Drosophila melanogaster by the uptake of plant- and animal?originated Na/K-ATPase inhibitors  [Not invited]
    Koji Kakutani
    Interuniversity Workshop on Biological Responses to External Stimuli  2015/12  大阪  Interuniversity Workshop on Biological Responses to External Stimuli
  • サフラン柱頭様組織の分化とクロシン産生  [Not invited]
    角谷晃司; 森健太郎; 石川理英; 安尾みなみ; 古川明典; 中村恭子; 福田浩三
    日本生薬学会第62年会  2015/09  岐阜  日本生薬学会第62年会
  • 植物工場に利用できる機能性および薬用植物の栽培・生産技術  [Not invited]
    角谷晃司
    最新研究から見る完全制御型植物工場における新しい植栽植物の可能  2015/08  東京
  • 植物工場における薬用植物栽培の可能性  [Not invited]
    角谷晃司
    平成２６年度 「第２回 植物工場研究会」  2015/01  新潟県工業技術総合研究所
  • A field investigation of the bryophyte distribution in the mountainous campus of Kinki University: Collection and cytological observation of liverworts as a potential source of nutraceuticals  [Not invited]
    瀧川 義浩; 角谷 晃司; 松田 克礼; 野々村 照雄; 豊田 秀吉
    MOSS 2011, The 14th annual international conference  2011/09  ドイツ  MOSS 2011, The 14th annual international conference
  • The flat liverwort thallus surface is the site of interactions with falling airborne fungal spores: Specification of conidial germination by powdery mildew on a thalloid surface  [Not invited]
    瀧川 義浩; 松田 克礼; 角谷 晃司; 野々村 照雄; 豊田 秀吉
    MOSS 2011, The 14th annual international conference  2011/09  ドイツ  MOSS 2011, The 14th annual international conference
  • Leaf surface of the moss is the site for trapping airborne fungal spores as nutritional targets  [Not invited]
    瀧川 義浩; 角谷 晃司; 松田 克礼; 野々村 照雄; 豊田 秀吉
    MOSS 2011, The 14th annual international conference  2011/09  ドイツ  MOSS 2011, The 14th annual international conference
  • ショウジョウバエを用いた各種ポリフェノール化合物の抗加齢効果の検討  [Not invited]
    角谷 晃司; 瀧川 義浩; 野々村 照雄; 松田 克礼; 豊田 秀吉; 早川 堯夫; 近畿大学大学院農学研究科
    第59回日本薬学会近畿支部例会  2009/10  大阪  第59回日本薬学会近畿支部例会
  • トマトに含まれるポリフェノール類の動物培養細胞に及ぼす影響  [Not invited]
    角谷 晃司; 瀧川 義浩; 早川 堯夫; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学農学部; 近畿大学農学部; 近畿大学農学部
    第59回日本薬学会近畿支部例会  2009/10  大阪  第59回日本薬学会近畿支部例会
  • 各種エリシター処理によるResveratrol生合成関連遺伝子の発現解析  [Not invited]
    角谷 晃司; 瀧川 義浩; 早川 堯夫; 近畿大学農学部; 近畿大学農学部
    日本農芸化学会2009年度大会  2009/03  福岡  日本農芸化学会2009年度大会
  • 既知ポリフェノール類のSIRT1活性促進効果  [Not invited]
    角谷 晃司; 瀧川 義浩; 早川 堯夫; 近畿大学農学部; 近畿大学農学部
    日本薬学会129年会  2009/03  京都  日本薬学会129年会
  • タバココナジラミ(バイオタイプQ)を用いた寿命検定法の開発  [Not invited]
    角谷 晃司; 瀧川 義浩; 近畿大学大学院農学研究科; 近畿大学大学院農学研究科; 近畿大学農学部; 近畿大学農学部
    日本農芸化学会2009年度大会  2009  福岡  日本農芸化学会2009年度大会
  • 大和シャクヤクの生長点培養における褐変化の抑制  [Not invited]
    角谷 晃司; 近畿大学薬学総合研究所; 福田商店
    第26回日本植物細胞分子生物学会大会  2008/09  大阪  第26回日本植物細胞分子生物学会大会
  • 大和シャクヤクの生長点培養  [Not invited]
    角谷 晃司; 近畿大学薬学総合研究所; 福田商店
    日本生薬学会第54回年会  2008/09  長崎  日本生薬学会第54回年会
  • 組織培養のおける大和シャクヤクの生長点組織の褐変化抑制  [Not invited]
    角谷 晃司; 近畿大学薬学総合研究所; 福田商店
    日本農芸化学会2008年度大会  2008/03  名古屋  日本農芸化学会2008年度大会
  • 冬虫夏草（Cordyceps sinensis）菌糸の分離と培養条件の検討  [Not invited]
    角谷 晃司; 瀧川 義浩; 森川 敏生; 二宮 清文; 村岡 修; 掛樋 一晃; 薬学部
    日本農芸化学会2008年度大会  2008  名古屋  日本農芸化学会2008年度大会
  • ＲＡＰＤ分析による大和トウキと北海トウキの識別  [Not invited]
    角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学農学部; 福田商店; 福田商店
    日本生薬学会第53回年会  2007/09  埼玉  日本生薬学会第53回年会
  • Resveratrol合成関連酵素を利用したResvertarolおよびその前駆体4-coumaroyl CoAの生合成  [Not invited]
    角谷 晃司; 瀧川 義浩; 松田 克礼; 豊田 秀吉; 野々村 照雄; 近畿大学大学院農学研究科
    日本生薬学会第54回年会  2007/09  名古屋  日本生薬学会第54回年会
  • わが国に自生するツユクサの遺伝子多型解析－オオボウシバナの識別  [Not invited]
    角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学農学部
    第２４回日本植物細胞分子生物学会（つくば）大会  2007/07  筑波  第２４回日本植物細胞分子生物学会（つくば）大会
  • トウキ・シャクヤクの遺伝子多型の解析  [Not invited]
    角谷 晃司
    第２４回日本植物細胞分子生物学会（つくば）大会  2007/07  筑波  第２４回日本植物細胞分子生物学会（つくば）大会
  • 腫瘍細胞表面で発現するCD98hc抗原領域の探索  [Not invited]
    角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉; 瀧川 義浩; 益子 高; 近畿大学大学院農学研究科
    2007年度日本農芸化学会大会  2007/03  東京  2007年度日本農芸化学会大会
  • 酵母細胞膜表面で固定化したResvertrol合成関連酵素による、 Resvertrolおよびその前駆体4-coumaroyl CoAの生合成  [Not invited]
    角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学大学院農学研究科; 近畿大学農学部
    2007年度日本農芸化学会大会  2007/03  東京  2007年度日本農芸化学会大会
  • アルギニントランスポーターを利用した出芽酵母細胞膜上における固定化酵素の作出  [Not invited]
    角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学大学院農学研究科
    第126回日本薬学会年会  2007/03  仙台  第126回日本薬学会年会
  • 高解像能デジタルマイクロスコープによるトマトうどんこ病菌分生胞子発芽の連続観察  [Not invited]
    角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学農学部; 近畿大学大学院農学研究科; 近畿大学農学部; 大阪府立農林技術センター
    平成17 年度日本植物病理学会大会  2006/03  静岡  平成17 年度日本植物病理学会大会
  • オムギうどんこ病菌による子葉鞘細胞において傷害刺激で誘導されるキチナーゼ遺伝子の能動的抑制  [Not invited]
    角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学農学部; 近畿大学農学部; 近畿大学農学部
    平成17 年度日本植物病理学会大会  2006/03  静岡  平成17 年度日本植物病理学会大会
  • エンド型およびエキソ型ポリガラクツロナーゼ遺伝子の塩基置換を利用したトマト根腐萎凋病菌菌株間での識別マーカーの開発  [Not invited]
    角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学農学部; 近畿大学農学部; 近畿大学農学部
    平成17 年度日本植物病理学会大会  2006/03  静岡  平成17 年度日本植物病理学会大会
  • Production of wholesome plants by introduction of genes for tumor-specific protein (CD98),  [Not invited]
    角谷 晃司; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学大学院農学研究科
    XVII Interntional Botanical Congress  2005/06  Austria  XVII Interntional Botanical Congress
  • ミヤコグサ（Lotus japonicus）におけるトランスポーター遺伝子の発現解析  [Not invited]
    角谷 晃司; 瀧川 義浩; 野々村 照雄; 松田 克礼; 豊田 秀吉; 近畿大学大学院農学研究科; 近畿大学農学部
    第45回日本植物生理学会年会  2004/08  第45回日本植物生理学会年会
  • ミヤコグサにおけるトランスポーター遺伝子の発現解析  [Not invited]
    角谷 晃司; 瀧川 義浩; 野々村 照雄; 松田 克礼; 豊田 秀吉; 益子 高; 近畿大学大学院農学研究科; 近畿大学農学部; 近畿大学農学部; かずさDNA研; かずさDNA研
    日本農芸化学学会平成15年度大会  2004/08  日本農芸化学学会平成15年度大会
  • トマト栽培品種における迅速かつ効率的な再分化条件の確立  [Not invited]
    角谷 晃司
    第２２回日本植物細胞分子生物学会  2004/08  秋田  第２２回日本植物細胞分子生物学会
  • ミヤコグサにおける硝酸およびアミノ酸トランスポーター遺伝子の発現解析  [Not invited]
    角谷 晃司
    第２２回日本植物細胞分子生物学会  2004/08  秋田  第２２回日本植物細胞分子生物学会
  • Biological control of powdery mildew and gray mold of tomato by chitinolytic phylloepiphytes ? Biological control of phyllophagous ladybird beetles of chitinolytic bacteria  [Not invited]
    豊田 秀吉; 松田 克礼; 藤原圭一; 野々村 照雄; 桜谷保之; 角谷 晃司; 大津康成; 土佐幸雄; 眞山滋志
    平成15 年度日本植物病理学会関西部会（奈良）  2003/10  平成15 年度日本植物病理学会関西部会（奈良）
     
    本実験では、トマトから葉圏に生息するキチナーゼ生産性細菌を分離し、ニジュウヤホシテントウの生物防除を試みた。まず、最も高いキチン分解活性を示す細菌KPM.012A を中腸ペリトロフィック膜に処理したところ、キチナーゼによる分解が確認された。
  • Biological control of powdery mildew and gray mold of tomato by chitinolytic phylloepiphytes ? Biological control of phyllophagous ladybird beetles by entomopathogenic bacteria  [Not invited]
    豊田 秀吉; 藤原圭一; 松田 克礼; 野々村 照雄; 桜谷保之; 角谷 晃司; 大津康成; 土佐幸雄; 眞山滋志
    平成15 年度日本植物病理学会関西支部（奈良）  2003/10  平成15 年度日本植物病理学会関西支部（奈良）
     
    本実験では、トマト葉圏から幼虫に対して食害抑制効果を示す細菌の分離を試み、生物防除資材への適用を検討した。トマト葉圏から分離された細菌を幼虫の経口から直接導入し、一定期間トマト葉を摂食させることにより食害抑制効果を示す細菌の選抜を行った。
  • Biological control of powdery mildew and gray mold of tomato by chitinolytic phylloepiphytes ? Stable phylloplane colonization by entomopathegenic bacterium Pseudomonas fluorescens KPM.018P  [Not invited]
    豊田 秀吉; 東希望; 松田 克礼; 藤原圭一; 野々村 照雄; 澤邊 昭義; 角谷 晃司; 大津康成; 土佐幸雄; 眞山滋志
    平成15 年度日本植物病理学会関西支部（奈良）  2003/10  平成15 年度日本植物病理学会関西支部（奈良）
     
    本実験では、KPM.18P の特性を調査し、さらに定着能力を強化することとした。一般に高度葉面定着を示す細菌は生物界面活性剤を生産することが報告されていることから、本細菌の生物界面活性剤生産能について検討した。
  • Produce of physiologically functional plants introduced the CD98 gene for tumor-specific protein  [Not invited]
    角谷 晃司; 豊田 秀吉; 森浦展行; 岩崎健太; 畑太一; 益子 高
    日本植物生理学会2003年度年会（大阪）  2003/03  日本植物生理学会2003年度年会（大阪）
     
    ガン細胞特異タンパク質であるCD98の遺伝子をミヤコグサに導入し、その遺伝子の発現とタンパク質の翻訳について報告した。これらの植物はガン予防を目的とした経口ワクチンとしての利用が示唆された。
  • Microinjection.based in situ PCR for gene cloning in targeted single cells of barley coleoptile（?）gene-expression detection mediated by RT.PCR of needle-sucked cell contents  [Not invited]
    豊田 秀吉; 松田 克礼; 鮫島武; 和夛泉季; 野々村 照雄; 藤田和久; 角谷 晃司
    平成15年度日本植物病理学会大会（東京）  2003/03  平成15年度日本植物病理学会大会（東京）
     
    オオムギうどんこ病菌の感染を受けた子葉鞘細胞およびその隣接細胞に単一細胞RT.PCR法を適用し、感染特異的に発現する遺伝子の解析を進めている。本研究では、単一細胞から細胞内容物を吸引し、そこに存在するmRNAを鋳型としたRT.PCRを試みた。
  • Suppression of Fusarium oxysporum by 5.fluoroindole（5.FI）and molecular breeding of 5.FI resistant plants（?）Cloning of modified anthranilate synthetase gene（masa）from Arabido psis thaliane  [Not invited]
    豊田 秀吉; 野々村 照雄; 福元健志; 松田 克礼; 小牧伸一郎; 角谷 晃司
    平成15年度日本植物病理学会大会（東京）  2003/03  平成15年度日本植物病理学会大会（東京）
     
    5.フルオロインドール（5.FI）耐性植物の作出を目的として、シロイヌナズナ（品種コロンビア）からアントラニル酸合成酵素遺伝子をクローニングした後、フィードバック阻害を受けない変異遺伝子の作製を試みた。
  • トマトにおけるレトロトランスポゾンの発現解析  [Not invited]
    角谷 晃司; 豊田 秀吉; 松田 克礼; 野々村 照雄; 南條綾子; 笹尾真理
    日本農芸化学関西支部大会（奈良）  2002/10  日本農芸化学関西支部大会（奈良）
     
    トマトのレトロトランスポゾンの発現をRT?PCR法により検出した。Ty1/copia型およびTy3/gypsy型レトロトランスポゾンの逆転写酵素領域は、葉ならびにカルス細胞で発現していた。
  • ガン特異的タンパクCD98遺伝子を導入した形質転換体の作出  [Not invited]
    角谷 晃司; 豊田 秀吉; 森浦展行; 岩崎健太; 畑太一; 益子 高
    日本農芸化学関西支部大会（奈良）  2002/10  日本農芸化学関西支部大会（奈良）
     
    ガン特異的タンパク質であるCD98の全長遺伝子を植物へ導入した。組み込まれたCD98遺伝子の発現をRT?PCR法で検出するとともに、発現タンパク質の生産をウエスタンブロット分析で調べた。
  • カンゾウの人工栽培におけるグリチルリチン生産  [Not invited]
    角谷 晃司; 友田勝巳; 尾崎和男; 渡
    生薬学会第49回年会（福岡）  2002/09  生薬学会第49回年会（福岡）
     
    カンゾウの培養苗を用いた人工栽培によるグリチルリチン生産を目的とし、水耕栽培に用いるロックウールや種々の支持体が、カンゾウの生育特性、根の肥大化ならびにグリチルリチン生産に及ぼす影響を検討した。
  • Pricking RT?PCR for cloning genes expressed in targeted infection structures of Blumeria graminis f. sp. hordei?  [Not invited]
    豊田 秀吉; 鮫島武; 松田 克礼; 藤田和久; 木多景子; 野々村 照雄; 角谷 晃司; 池田成志
    平成14年度日本植物病理学会関西部会（三重）  2002/09  平成14年度日本植物病理学会関西部会（三重）
     
    本菌は絶対寄生菌であることから、特定の分子ステージにあるうどんこ病菌を大量に得ることは非常に困難であり、それぞれの器官で発現する遺伝子の解析が必ずしも容易ではない。本実験ではPricking RT?PCR法を用い、単一器官を標的として発現遺伝子の解析を行った。
  • トマトにおけるTy1/copia型およびTy3/gypsy型レトロトランスポゾンのクローニング  [Not invited]
    角谷 晃司; 豊田 秀吉; 松田 克礼; 野々村 照雄; 南條綾子; 笹尾真理; 玉井智子
    日本育種学会第102回年会（帯広）  2002/08  日本育種学会第102回年会（帯広）
     
    トマトのレトロトランスポゾンをゲノムからクローニングし、その遺伝子解析を試みた。ゲノム上には多数のレトロトランスポゾンが存在しており、また、培養栽培において発現している新規のTy1/copia型レトロトランスポゾンをクローニングした。
  • トマトレトロトランスポゾンの発現解析  [Not invited]
    角谷 晃司; 豊田 秀吉; 松田 克礼; 野々村 照雄; 南條綾子; 中川真樹; 笹尾真理
    第20回日本植物細胞分子生物学会大会（奈良）  2002/07  第20回日本植物細胞分子生物学会大会（奈良）
     
    Ty1/copia型およびTy3/gypsy型レトロトランスポゾンの逆転写酵素領域で保存されているアミノ酸配列からプライマーを合成し、RT?PCR分析に用いた。トマト葉およびカルスにおいて、これらは発現しており、既知Ty1/copia型とは異なる断片がカルスより増幅した。
  • ガン特異的タンパクCD98遺伝子を導入した形質転換体の作出  [Not invited]
    角谷 晃司; 豊田 秀吉; 松田 克礼; 野々村 照雄; 森浦展行; 益子 高
    第20回日本植物細胞分子生物学会大会（奈良）  2002/07  第20回日本植物細胞分子生物学会大会（奈良）
     
    経口ガンワクチンの開発を目的とし、ガン細胞特異的タンパク質CD98遺伝子を植物へ導入した。その遺伝子発現をRT?PCRで検出し、さらにタンパク質の翻訳をウエスタンブロット分析で調べた。その結果、組換え植物はCD98タンパク質を生産しており経口ワクチンの可能性が示唆された。
  • Microinjection based in situ PCR for gene cloning in targeted single cells of barley coleoptile （?） Control of gene amplification by different primers and annealing conditions  [Not invited]
    豊田 秀吉; 平井康晴; 松田 克礼; 相場政人; 藤田和久; 野々村 照雄; 角谷 晃司
    平成 13 年度日本植物病理学会関西部会 （高知）  2001/10  平成 13 年度日本植物病理学会関西部会 （高知）
     
    オオムギ子葉鞘の単一細胞から RT PCR により遺伝子をクローニングするため、 基礎的条件として常時発現している遺伝子のプライマーを構築し、 PCR 時の反応温度および増幅量について検討した。
  • カンゾウ培養細胞で発現している配糖化酵素遺伝子の解析  [Not invited]
    角谷 晃司; 黄鵬宇; 林宏明; 井上謙一郎
    日本生薬学会第 48 回年会 （金沢）  2001/09  日本生薬学会第 48 回年会 （金沢）
     
    カンゾウ培養細胞で発現している配糖化酵素遺伝子のスクリーニングを行った。 すなわち、 肥大根で発現している RGUTI 遺伝子をプローブとして cDNA ライブラリーをスクリーニングしたところ、 3 種の新規なクローンを分離し、 その機能と解析を報告した。
  • カンゾウ属植物の RAPD 分析による分類 特に Glycyrrhiza glabra L. 種と G. uralensis F. 種の識別  [Not invited]
    角谷 晃司; 尾崎和男; 渡
    日本生薬学会第 48 回年会 （金沢）  2001/09  日本生薬学会第 48 回年会 （金沢）
     
    RAPD 分析により 5 種の甘草属植物を分類することができた。 G. glabra 種と G. uralensis 種のグループと G. echirata 種、 G. pallidiflora 種および G. squamulosa 種のグループに分類され、 両者はそれぞれ近縁種であることが判明した。
  • Purification and property of UDP glucuronosyltransferase in the root of the licorice （Glycyrrhiza glabra L.）  [Not invited]
    角谷 晃司
    第 19 回日本植物細胞分子生物学会 （東京）  2001/07  第 19 回日本植物細胞分子生物学会 （東京）
     
    カンゾウの薬用成分であるグリチルリチンの生合成を明らかにするため、 それらに関与するグルクロン酸転移酵素を生根より抽出、 精製した。 約 50 KPa の分子量のタンパク質であり、 作用 PH が異なる 2 種のタンパクがグリチルリチン生合成に関与していた。
  • 甘草のグリチルリチン生産に関する基礎的研究と応用的アプローチ  [Not invited]
    角谷 晃司
    甘草に関するシンポジウム （塩山）  2001/07  甘草に関するシンポジウム （塩山）
     
    甘草のグリチルリチンに関する生合成メカニズムや遺伝子機能等の基礎的研究からグリチルリチンの生産に至るまでの応用的研究について述べた。
  • Purification and characterization of UDP glucuronosyl transferase of licorice  [Not invited]
    角谷 晃司; 友田勝巳; 渡辺斉
    日本農芸化学会 2001 年度大会 (京都)  2001/03  日本農芸化学会 2001 年度大会 (京都)
     
    甘草の主成分であるグリチルリチンは､ グリチルレチン酸の 3 位にグルクロン酸 2 分子が結合したトリテルペンサボニンである｡ 今回､ グリチルリチン生合成に関与するグルクロン酸転移酵素を生根より精製し､ その諸性質について詳細な検討を試みた｡

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