KINDAI UNIVERSITY


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KAKUTANI Koji

Profile

FacultyPharmaceutical Research and Technology Institute
PositionProfessor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/819-kakutani-kouji.html
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Last Updated :2020/09/02

Research Activities

Research Areas

  • Life sciences, Environmental and pharmaceutical development resources, Pharmacognosy

Research Interests

  • Biological and Environmental Engineers, Plant Cell Biology, pharmacognosy

Published Papers

  • Quantitative analysis of the lifelong production of conidia released from single colonies of Podosphaera xanthii on melon leaves using electrostatic techniques, T. SuzukiR, NakamuraN, TakagiY. TakikawaK, KakutaniY. MatsudaK, MatsuiT. Nonomura, 48(3), 297 - 307, May 2019 , Refereed
  • A Simple Electrostatic Device for Eliminating Tobacco Sidestream Smoke to Prevent Passive Smoking, Yoshinori Matsuda, Koji Kakutani, Teruo Nonomura, Yoshihiro Takikawa, Kiyotsugu Okada, Manabu Shibao, Kazuhiro Miyama, Shinya Yokoo, Shin-ichi Kusakari, Hideyoshi Toyoda, Instrument, Instrument, 2(13), 1 - 10, Jun. 2018 , Refereed
  • Electrocution of mosquitoes in a piggery by a novel electrostatic window screen to minimize mosquito transmission of Japanese encephalitis virus, Kakutani K, Matsuda Y, Takikawa Y, Nonomura T, Okada K, Shibao M, Kusakari S, Miyama K, Toyoda H, International Journal of Scientific Research, International Journal of Scientific Research, 7(5), 47 - 50, May 2018 , Refereed
  • Selective electrostatic eradication of Sitophilus oryzae nesting in stored rice., Yoshinori Matsuda, Yoshihiro Takikawa, Teruo Nonomura, Koji Kakutani, Kiyotsugu Okada, Manabu Shibao, Shin-ichi Kusakari, Kazuhiro Miyama, Hideyoshi Toyoda, Journal of Food Technology and Preservation, Journal of Food Technology and Preservation, 2(1), 15 - 20, Apr. 2018 , Refereed
  • Successful single-truss cropping cultivation of healthy tomato seedlings raised in an electrostatically guarded nursery cabinet with non-chemical control of whiteflies., Kakutani K, Matsuda Y, Nonomura T, Takikawa Y, Okada K, Shibao M, Kusakari S, Toyoda H, Global Journal of Pests, Diseases and Crop Protection, Global Journal of Pests, Diseases and Crop Protection, 5(4), 269 - 275, Jul. 2017 , Refereed
  • An electrostatic-barrier-forming window that captures airborne pollen grains to prevent pollinosis, Takikawa Y, Matsuda Y, Nonomura T, Kakutani K, Kusakari S, Toyoda H, International Journal of Environmental Research and Public Health, International Journal of Environmental Research and Public Health, 14(82), 1 - 5, Feb. 2017 , Refereed
  • Electrostatic elimination of fine smoke particles by a newly devised air purification screen, Takikawa Y, Matsuda Y, Nonomura T, Kakutani K, Kusakari S, Toyoda H, International Journal of Scientific Research in Agricultural Sciences, International Journal of Scientific Research in Agricultural Sciences, 5(1), 17 - 21, Feb. 2017 , Refereed
  • Elimination of whiteflies colonising greenhouse tomato plants using an electrostatic flying insect catcher., Takikawa Y, Matsuda Y, Nonomura T, Kakutani K, Okada K, Shibao M, Kusakari S, Toyoda H, International Journal of Current Advanced Research, International Journal of Current Advanced Research, 6(8), 5517 - 5521, 2017 , Refereed
  • An electrostatic nursery shelter for raising pest and pathogen free tomato seedlings in an open-window greenhouse environment, Takikawa Y, Matsuda Y, Nonomura T, Kakutani K, Okada K, Morikawa S, Shibao M, Kusakari S, Toyoda H, Journal of Agricultural Science, Journal of Agricultural Science, 8, 13 - 25, 2016 , Refereed
  • Discrimination of Salacia chinensis Based on the DNA Sequence of the rDNA ITS Region, 中村恭子, 赤木淳二, 赤木淳二, 石伏史明, 谷恭輔, 森川敏生, 森川敏生, YUTANA Pongpiriyadacha, 村岡修, 村岡修, 村岡修, 早川尭夫, 角谷晃司, 角谷晃司, 生薬学雑誌, 生薬学雑誌, 69(2), 53 - 58, Aug. 20 2015 , Refereed
  • Studies on conditions for breaking dormancy of Yamato peony (Paeonia lactiflora Pallas var. trichocarpa (Bunge) Stern) and its artificial cultivation, 中村恭子, 大野加奈, 森健太郎, 瀧川義浩, 角谷晃司, 薬用植物研究, 薬用植物研究, 37(1), 22 - 28, Jun. 18 2015 , Refereed
  • Development of an electrostatic trap with an insect discharge recorder for multiple real-time monitoring of pests prowling in a warehouse, Takikawa Y, Matsuda Y, Nonomura T, Kakutani K, Kusakari S, Toyoda H, International Journal of Advance Agricultural Research, International Journal of Advance Agricultural Research, 3, 55 - 63, 2015 , Refereed
  • Prevention of whitefly entry from a greenhouse entrance by furnishing an airflow-oriented pre-entrance room guarded with electric field screens, T. Nonomura, Y. Matsuda, K. Kakutani, Y. Takikawa, J. Kimbara, K. Osamura, S. Kusakari, H. Toyoda, Journal of Agricultural Science, Journal of Agricultural Science, 6, 172 - 184, Nov. 2014 , Refereed
  • Successional changes in powdery mildew pathogens prevailing in common and wild tomato plants rotation-cultivated in a greenhouse, T. Nonomura, Y. Matsuda, Y. Takikawa, K. Kakutani, H. Toyoda, Ann. Rept. Kansai Pl. Prot, Ann. Rept. Kansai Pl. Prot, 56, 17 - 20, 2014 , Refereed
  • 近畿大学農学部実験温室におけるトマト黄化葉巻病の発生とタバココナジラミバイオタイプの検定, S. Kusakari, H. Toyoda, 近畿大学農学部紀要, 近畿大学農学部紀要, 46, 55 - 58, 2013
  • Practical Application of an Electric Field Screen to an Exclusion of Flying Insect Pests and Airborne Fungal Conidia from Greenhouses with a Good Air Penetration, Kakutani K, Matsuda Y, Nonomura T, Kimbara J, Kusakari S, Toyoda H, Journal of Agricultural Science, Journal of Agricultural Science, 4, 51 - 60, 2012 , Refereed
  • Pests and Airborne Fungal Conidia from Greenhouses with a Good Air Penetration., Kusakari S, Toyoda H, Eur. J. Plant Pathol., Eur. J. Plant Pathol., 134, 661 - 670, 2012 , Refereed
  • of their negative charge, Osamura K, Toyoda H, Journal of Agricultural Science, Journal of Agricultural Science, 4, 51 - 60, 2012 , Refereed
  • An Electric Field Screen can Create Pest-free Space with Better Air Penetration in Open-window Greenhouses, An Electric Field, Screen can Create Pest-free Space with Better Air Penetration in, Open-window Greenhouses, Acta Horticulturae, Acta Horticulturae, 952, 559 - 566, 2012 , Refereed
  • Direct RT-PCR amplification of mature mRNA in cytoplasm micropipetted from barley coleoptile epidermal cell ? A model system for analyzing gene expression in host cells attacked by powdery., 豊田 秀吉, 松田 克礼, 角谷 晃司, Mem. Fac. Agr. Kinki Univ, Mem. Fac. Agr. Kinki Univ, 36, 9 - 18, Mar. 2005
    Summary:本実験では、顕微鏡下で標的とした単一細胞からマイクロピペットを用いて細胞内容物を吸引し、そこに存在するmRNAを鋳型とした単一細胞RT-PCR法を適用することとした。病原菌の感染によって発現が誘導される遺伝子(CHI2、GLUなど)を検索した。誘導型遺伝子の発現を厳密に評価するため、RT-PCRを行う際に、先の構成的発現遺伝子を増幅するプライマーを混合し、指標遺伝子として同時に増幅することにより、誘導型遺伝子の発現検出を行った。その結果、病原菌の感染を受けていない細胞およびその感染を受けている両者の細胞において指標とした構成的発現遺伝子は検出された。誘導型遺伝子として使用したCHI2遺伝子およびGLU遺伝子は病原菌の感染を受けていない細胞においてその発現が検出され、うどんこ病菌の感染過程において抑制される傾向にあった。
  • Analysis of UDP.glucuronosyltrans ferase gene of Glycyrrhiza glabra L., 角谷 晃司, 薬学総合研究所紀要, 薬学総合研究所紀要, 11, 105 - 110, Mar. 2003
    Summary:ディデネレートプライマーを利用したRT.PCR法により、甘草根のtotal RNAから糖転移酵素遺伝子を増幅した。この遺伝子はエンドウのグルクルタン酸転移酵素遺伝子と高い相同性が示され、甘草のグリチルチリン生合成に関与する糖転移酵素遺伝子であると示唆された。
  • Rapid Detection of Chitosanase Activity in Chitosanase Gene-Transformed Strain of Enterobacter cloacae by Lytic Infection of Specific Bacteriophages, 角谷 晃司, 松田 克礼, 野々村 照雄, 豊田 秀吉, 近畿大学大学院農学研究科, 近畿大学大学院農学研究科, J. Gen. Plant Pathol, J. Gen. Plant Pathol, 69, 131 - 137, 2003 , Refereed
  • Infectivity of a Japanese isolate of Oidium neolycopersici KTP-01 to a European tomato cultivar resistant to O. lycopersici., 角谷 晃司, 松田 克礼, 野々村 照雄, 豊田 秀吉, 近畿大学大学院農学研究科, 近畿大学大学院農学研究科, 近畿大学大学院農学研究科, 近畿大学大学院農学研究科, 大阪府環境農林水産総合研究所, J. Gen. Plant Pathol, J. Gen. Plant Pathol, 69, 406 - 408, 2003 , Refereed
  • Efficient Release of Overproduced Gene Products from Escherichia coli BL21(DE3) by Lytic Infection with Newly Isolated Bacteriophages, 角谷 晃司, 松田 克礼, 野々村 照雄, 豊田 秀吉, 近畿大学大学院農学研究科, 近畿大学大学院農学研究科, Biosci. Biotech. Biochem., Biosci. Biotech. Biochem., 67(1), 198 - 202, Jan. 2003 , Refereed
  • Classification of Glycyrrhiza Plants by Random Amplified Polymorphic DNA analysis Discrimination of Glycyrrhiza glabra L. and G. uralensis Fischer, 角谷 晃司, 友田勝巳, 尾崎和男, 芝野真喜雄, 草野源次, Natural Medicines, Natural Medicines, 55(6), 287 - 293, 2001 , Refereed
    Summary:RAPD 分析により 5 種のカンゾウ属植物の分類を行うことができた。 さらに、 G. glabra と G. uralensis において特異的な PCR 増幅断片が得られ、 その塩基配列をもとに作製 RAPD マーカーは、 両者を効率的に識別することができた。
  • Classification of Glycyrrhiza Plants by Random Amplified Polymorphic DNA analysis Discrimination of Glycyrrhiza glabra L. species and G. uralensis F. species, 角谷 晃司, 友田勝巳, 薬学総合研究所紀要, 薬学総合研究所紀要, (9), 95 - 102, Jan. 2001
    Summary:RAPD 分析により 5 種の異なる甘草を分類することが可能になった。 さらに、 本分析法により得られた種特異的断片の塩基配列より、 これまで識別が困難であった G. glabra 種と G. uralensis 種を区別できる RAPD マーカーを作製することができた。
  • Effects of light quality on conidiophore formation of the melon powdery mildew pathogen Podosphaera xanthii, Tomoko Suzuki, Shougo Nishimura, Kazuhiro Yagi, Ryousuke Nakamura, Yoshihiro Takikawa, Yoshinori Matsuda, Koji Kakutani, Teruo Nonomura, Phytoparasitica, Phytoparasitica, 46(1), 31 - 43, Feb. 01 2018
    Summary:The lengths of conidiophores in fungal colonies of the melon powdery mildew pathogen Podosphaera xanthii Pollacci KMP-6 N cultured under greenhouse (natural) conditions differed markedly from those cultured in a growth chamber. We hypothesized that light wavelength was responsible for the differences in conidiophore length. In this study, we examined the effects of light-emitting diode (LED) irradiation (purple, blue, green, orange, and red light) and white light on colony development and conidiophore formation in KMP-6 N using a stereomicroscope and a high-fidelity digital microscope. Colonies on leaves were flat under greenhouse conditions and under red LED light irradiation but were stacked under growth chamber conditions and under purple, blue, green, and orange LED light irradiation. In addition, KMP-6 N formed catenated conidia comprising six conidia per conidiophore under greenhouse conditions and red light but more than seven conidia per conidiophore under growth chamber conditions and purple, blue, green, and orange light. Furthermore, almost none of the conidia on top of the conidiophores grown under blue light were fully constricted. Therefore, these fungi could not scatter their conidia and spread infection. This is the first report of the effects of LED lights on conidiophore formation in the melon powdery mildew fungus P. xanthii. The results provide insight into the mechanisms underlying the responses of conidiophores to light of specific wavelengths and conidial scatter from conidiophores of melon powdery mildew fungi.
  • Trichomes: interaction sites of tomato leaves with biotrophic powdery mildew pathogens, Tomoko Suzuki, Tomoe Murakami, Yoshihiro Takizumi, Hiroyuki Ishimaru, Daiki Kudo, Yoshihiro Takikawa, Yoshinori Matsuda, Koji Kakutani, Yuling Bai, Teruo Nonomura, European Journal of Plant Pathology, European Journal of Plant Pathology, 150(1), 115 - 125, Jan. 01 2018
    Summary:The present study aimed to explore the possibility of using the type I trichomes of tomato (Solanum lycopersicum) to monitor the infection processes of powdery mildews by microscopy. Individual trichome cells of two tomato genotypes were inoculated with pathogenic and non-pathogenic powdery mildew species, Pseudoidium neolycopersici, Erysiphe trifoliorum and Podosphaera xanthii. On the trichome cells of the tomato cultivar Moneymaker, hyphae of the pathogenic Pseudoidium neolycopersici (isolates KTP-03 and KTP-04) grew vigorously whereas hyphal growth of the non-pathogenic Erysiphe trifoliorum and Podosphaera xanthii ceased after appressorium formation, which was associated with papilla formation and hypersensitive cell death, respectively. Similar infection processes of the tested powdery mildews were seen in Moneymaker epidermal cells. Therefore, tomato trichomes are suitable for analysing, at individual cell level, the infection processes of different pathotypes of powdery mildews and for observing the cytological responses of plants by non-pathogenic powdery mildews. On the other hand, it was observed that both isolates KTP-03 and KTP-04 failed to produce conidiophores on the hyphae elongating on Moneymaker trichomes. Similarly, no conidiophores were produced on the hyphae elongating on trichomes of Solanum peruvianum LA2172, which is resistant to KTP-03 and susceptible to KTP-04. Interestingly, delayed cell death occurred in LA2172 epidermal cells, which were attacked by KTP-03 hyphae elongating from trichomes and conidiophores were formed on new hyphae growing from the leaf epidermal cells. Thus, leaf trichomes and epidermal cells of the wild tomato species LA2172 reacted differently to the avirulent isolate KTP-03.
  • An electrostatic-barrier-forming window that captures airborne pollen grains to prevent pollinosis, Yoshihiro Takikawa, Yoshinori Matsuda, Teruo Nonomura, Koji Kakutani, Shin-Ichi Kusakari, Hideyoshi Toyoda, International Journal of Environmental Research and Public Health, International Journal of Environmental Research and Public Health, 14(1), Jan. 15 2017
    Summary:An electrostatic-barrier-forming window (EBW) was devised to capture airborne pollen, which can cause allergic pollinosis. The EBW consisted of three layers of insulated conductor wires (ICWs) and two voltage generators that supplied negative charges to the two outer ICW layers and a positive charge to the middle ICW layer. The ICWs generated an attractive force that captured pollen of the Japanese cedar, Cryptomeria japonica, from air blown through the EBW. The attractive force was directly proportional to the applied voltage. At ≥3.5 kV, the EBW exerted sufficient force to capture all pollen carried at an air flow of 3 m/s, and pollen-free air passed through the EBW. The findings demonstrated that the electrostatic barrier that formed inside the EBW was very effective at capturing airborne pollen thus, it could allow a home to remain pollen-free and healthy despite continuous pollen exposure.
  • Micropipette extraction-based PCR amplification of mature mRNAs in single trichome cells of tomato leaves, T. Nonomura, Y. Matsuda, H. Toyoda, Y. Takikawa, K. Kakutani, INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY AND OTHER OMICS IN VEGETABLE SCIENCE, INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY AND OTHER OMICS IN VEGETABLE SCIENCE, 1145, 31 - 37, 2016 , Refereed
    Summary:Single-cell polymerase chain reaction (PCR) was conducted to detect in situ gene expression in targeted cells of tomato leaf trichomes. The cytoplasm was removed with a micropipette under a light microscope and subsequently used for reverse transcription PCR (RT-PCR), followed by nested PCR. Two intron-containing genes, a glyceraldehyde 3-phosphate dehydrogenase gene and a plasma membrane H+-ATPase gene, were constantly expressed in these cells and therefore used as indicators of successful PCR reactions. In addition, the use of nucleus-free cellular contents for the RT-PCR and subsequent nested PCR analyses was effective for preventing contamination with the products derived from misamplification of corresponding genomic DNA sequences. Using this method, we detected the expression of certain stimuli-activated genes, following the exposure of trichome cells to volatile chemicals. Therefore, the present technique can be used to directly detect gene expression in single trichome cells of tomato leaves in response to external stimulation.
  • Micropipette extraction-based PCR amplification of mRNAs in single friable tomato callus cells, Y. Takikawa, H. Toyoda, Y. Matsuda, T. Nonomura, K. Kakutani, INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY AND OTHER OMICS IN VEGETABLE SCIENCE, INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY AND OTHER OMICS IN VEGETABLE SCIENCE, 1145, 23 - 29, 2016 , Refereed
    Summary:A microscopy-based needle micromanipulation technique for direct injection of foreign material into target cells was refined as a novel method of aspiration of cellular contents from a single target cell using a micropipette. Subsequently, we performed direct PCR amplification of mRNAs transcribed in target tomato callus cells. Friable calli were induced from leaf explants, and single cells from active small cell aggregates were used as targets for aspiration. The aspirated cellular contents were subjected to RT-PCR and subsequent nested PCR amplification analyses. Five tomato genes (LHA2, GAPDH, CHI3, PI2 and TLC1) were selected from the cDNA database as PCR targets. The GAPDH and LHA2 genes were amplified from all aspirated samples and were used as indicators of successful PCR amplification. Aspiration of only the cytosol (excluding the nucleus), facilitated amplification of mature target gene mRNA that was not contaminated with PCR products derived from the genomic DNA sequences of the target genes. We identified novel stimulus activated genes, such as CHI3 and TLC1, which were constitutively transcribed in tomato callus cells.
  • Agrobacterium-mediated T-DNA insertion for mutagenesis assessed by the production of hairy roots emitting melon fruit odours, K. Kakutani, Y. Matsuda, T. Nonomura, H. Toyoda, Y. Takikawa, INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY AND OTHER OMICS IN VEGETABLE SCIENCE, INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY AND OTHER OMICS IN VEGETABLE SCIENCE, 1145(1145), 17 - 22, 2016 , Refereed
    Summary:This study examined whether Agrobacterium-mediated T-DNA insertion was useful for obtaining mutant melon plants. For this purpose, we used Agrobacterium rhizogenes because of its frequent hairy root production when inoculated into plants and the easy detection of fruit odours produced by the hairy roots. Of the 6534 hairy root clones obtained, five clones were mutants emitting fruit odours, and the KMH-4196 clone produced the strongest melon fruit odours. These odours were due to the production of at least four compounds that were not detected in non-aromatic hairy root clones: (Z)-3-hexenol, (E)-2-hexenal, 1-nonanoland (Z)-6-nonenol. (Z)-6-nonenol was the most important and was produced stably during successive subculture for 3 years. Molecular analyses indicated that a single copy of T-DNA from the inoculated bacteria was integrated into a chromosome in the clone, suggesting that Agrobacterium-mediated T-DNA insertion is an effective mutagenesis method in higher plants.
  • Defence responses of Aphanoregma patens (Hedw.) Lindb. to inoculation with Pythium aphanidermatum (vol 37, pg 1, 2015), Yoshihiro Takikawa, Satomi Kida, Fuji Asayama, Teruo Nonomura, Yoshinori Matsuda, Koji Kakutani, Hideyoshi Toyoda, JOURNAL OF BRYOLOGY, JOURNAL OF BRYOLOGY, 37(3), 250 - 250, Sep. 2015 , Refereed
  • Digital microscopic analysis of conidiogenesis of powdery mildew pathogens isolated from melon leaves, Yoshihiro Takikawa, Teruo Nonomura, Shouta Miyamoto, Naoki Okamoto, Tomoe Murakami, Yoshinori Matsuda, Koji Kakutani, Shin-ichi Kusakari, Hideyoshi Toyoda, PHYTOPARASITICA, PHYTOPARASITICA, 43(4), 517 - 530, Sep. 2015 , Refereed
    Summary:Melons (Cucumis melo L.) grown hydroponically in a greenhouse were heavily infested with powdery mildew. We isolated powdery mildew pathogens from the melon leaves and identified the isolate as Podosphaera xanthii KMP-6N, based on morphological characteristics and sequences of ribosomal DNA internal transcribed spacer (rDNA-ITS) regions. Host ranges of KMP-6N were determined by estimating the infectivity or pathogenicity after inoculating the conidia onto multiple plant species. The fungi caused severe powdery mildew symptoms on Cucurbitaceae plants, producing scattered conidia on conidiophores. The goal of this study was to observe KMP-6N conidiogenesis on melon leaves. The pathogen formed completely catenated conidiophores approximately 24 h from conidiophore erection to release of mature conidia. Six conidia were produced on the conidiophores and only the conidia at the apex reached maturity. The cycles of conidial release were repeated on melon leaves 14 to 18 times, at approximately 6-h intervals. In the final stage, conidia were released without causing growth and septation of generative cells. Conidiophores produced an average of 36 conidia during a 90-h period. In our study, the modes of conidiogenesis, lifetime of conidiophores and productivity of conidia on a conidiophore were described for powdery mildew fungi.
  • Electrostatic insect sweeper for eliminating whiteflies colonizing host plants: A complementary pest control device in an electric field screen-guarded greenhouse, Yoshihiro Takikawa, Yoshinori Matsuda, Koji Kakutani, Teruo Nonomura, Shin-Ichi Kusakari, Kiyotsugu Okada, Junji Kimbara, Kazumi Osamura, Hideyoshi Toyoda, Insects, Insects, 6(2), 442 - 454, May 12 2015 , Refereed
    Summary:Our greenhouse tomatoes have suffered from attacks by viruliferous whiteflies Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) over the last 10 years. The fundamental countermeasure was the application of an electric field screen to the greenhouse windows to prevent their entry. However, while the protection was effective, it was incomplete, because of the lack of a guard at the greenhouse entrance area in fact, the pests entered from the entrance door when workers entered and exited. To address this, we developed a portable electrostatic insect sweeper as a supplementary technique to the screen. In this sweeper, eight insulated conductor wires (ICWs) were arranged at constant intervals along a polyvinylchloride (PVC) pipe and covered with a cylindrical stainless net. The ICWs and metal net were linked to a DC voltage generator (operated by 3-V alkaline batteries) inside the grip and oppositely electrified to generate an electric field between them. Whiteflies on the plants were attracted to the sweeper that was gently slid along the leaves. This apparatus was easy to operate on-site in a greenhouse and enabled capture of the whiteflies detected during the routine care of the tomato plants. Using this apparatus, we caught all whiteflies that invaded the non-guarded entrance door and minimized the appearance and spread of the viral disease in tomato plants in the greenhouse.
  • Defence responses of Aphanoregma patens (Hedw.) Lindb. to inoculation with Pythium aphanidermatum, Yoshihiro Takikawa, Satomi Kida, Fuji Asayama, Teruo Nonomura, Yoshinori Matsuda, Koji Kakutani, Hideyoshi Toyoda, JOURNAL OF BRYOLOGY, JOURNAL OF BRYOLOGY, 37(1), 1 - 7, Mar. 2015 , Refereed
    Summary:We examined the defensive responses of leaf surfaces, rhizoids, and protonemata of the moss Aphanoregma patens (Hedw.) Lindb. to inoculation with zoospores and encysted zoospores (cysts) of the oomycete Pythium aphanidermatum OPU849. Aphanoregma patens infected with Pythium exhibited extensive browning of the leaves and stems 4 days after inoculation. The zoospore infection sites were rhizoids and protonemata. Cysts on the rhizoids and protonemata germinated and formed elongated hyphae that invaded the cells of the moss, forming oospores and lobed sporangia. The subsequent production of new zoospores on infected plants indicates that A. patens is a suitable host for manipulating the life cycle of Pythium. However, cysts inoculated onto leaf surfaces did not germinate, and they began to show signs of disruption after only 3 hours. After 2 days, more than 80% of the cysts on leaf surfaces were completely disrupted, possibly due to the secretion of degrading enzymes. Cyst disruption was not observed on rhizoids nor on protonemata.
  • Avoidance of an electric field by insects: Fundamental biological phenomenon for an electrostatic pest-exclusion strategy, Y. Matsuda, T. Nonomura, K. Kakutani, J. Kimbara, K. Osamura, S. Kusakari, H. Toyoda, ELECTROSTATICS 2015, ELECTROSTATICS 2015, 646, 12003, 2015 , Refereed
    Summary:An electric field screen is a physical device used to exclude pest insects from greenhouses and warehouses to protect crop production and storage. The screen consists of iron insulated conductor wires (ICWs) arrayed in parallel and linked to each other, an electrostatic DC voltage generator used to supply a negative charge to the ICWs, and an earthed stainless net placed on one side of the ICW layer. The ICW was negatively charged to polarize the earthed net to create a positive charge on the ICW side surface, and an electric field formed between the opposite charges of the ICW and earthed net. The current study focused on the ability of the screen to repel insects reaching the screen net. This repulsion was a result of the insect's behaviour, i.e., the insects were deterred from entering the electric field of the screen. In fact, when the screen was negatively charged with the appropriate voltages, the insects placed their antennae inside the screen and then flew away without entering. Obviously, the insects recognized the electric field using their antennae and thereby avoided entering. Using a wide range of insects and spiders belonging to different taxonomic groups, we confirmed that the avoidance response to the electric field was common in these animals.
  • Safe housing ensured by an electric field screen that excludes insect-net permeating haematophagous mosquitoes carrying human pathogens, Y. Matsuda, K. Kakutani, T. Nonomura, J. Kimbara, K. Osamura, S. Kusakar, H. Toyoda, ELECTROSTATICS 2015, ELECTROSTATICS 2015, 646, 12002, 2015 , Refereed
    Summary:An electric field screen can be used to keep mosquitoes out of houses with open windows. In this study, doubly charged dipolar electric field screens (DD-screens) were used to capture mosquitoes entering through a window. The screen had two components: three layers of insulated conductor iron wires (ICWs) in parallel arrays and two electrostatic direct current (DC) voltage generators that supplied negative or positive voltages to the ICWs. Within each layer, the ICWs were parallel at 5-mm intervals, and connected to each other and to a negative or positive voltage generator. The negatively and positively charged ICWs are represented as ICW(-) and ICW(+), respectively. The screen consisted of one ICW(+) layer with an ICW(-) layer on either side. The Asian tiger mosquito (Aedes albopictus) and house mosquito (Culex pipiens) were used as models of vectors carrying viral pathogens. Adult mosquitoes were blown into the space between the ICWs by sending compressed air through the tip of an insect aspirator to determine the voltage range that captured all of the test insects. Wind speed was measured at the surface of the ICW using a sensitive anemometer. The result showed that at >= 1.2 kV, the force was strong enough that the ICWs captured all of the mosquitoes, despite a wind speed of 7 m/s. Therefore, the DD-screen could serve as a physical barrier to prevent noxious mosquitoes from entering houses with good air penetration.
  • Electrostatic guarding of bookshelves for mould-free preservation of valuable library books, Yoshihiro Takikawa, Yoshinori Matsuda, Teruo Nonomura, Koji Kakutani, Junji Kimbara, Kazumi Osamura, Shin-ichi Kusakari, Hideyoshi Toyoda, AEROBIOLOGIA, AEROBIOLOGIA, 30(4), 435 - 444, Dec. 2014 , Refereed
    Summary:Old books are highly susceptible to mould infection, and an effective method for avoiding moulding is needed to safely preserve valuable books in library stack rooms. Guarding a bookshelf with an electric field screen is a physical method that prevents airborne spores from entering the space used for book preservation. In this study, insulated conductor wires (ICWs) were used as electrodes to form electric fields. The ICWs were arrayed in parallel and linked to each other and to a direct current voltage generator. The electric field screen consisted of two layers of ICWs, which were negatively and positively charged with equal voltages to make dipoles, ICW(-) and ICW(+). Both ICWs generated an attractive force that captured airborne spores of Penicillium digitatum that were blown inside the screen. The attractive force was directly proportional to the applied voltage. At a parts per thousand 0.9 kV, the screen exerted sufficient force to capture all airflow-carried spores, but a few spores that were once captured were repulsed out of the electric field when subsequent spores were attracted to positions proximal to them. This phenomenon was explained by creeping discharge between spores located close to each other on the ICW surface. This spore-repulsion problem was resolved by adding an additional ICW layer to the electric field screen, namely an electric field screen with an ICW(-) layer on both sides of an ICW(+) layer. The present study demonstrated that the three-layered electric field screen remained mould-free inside a screen-guarded bookshelf, irrespective of continuous spore exposure.
  • Electrostatic measurement of dischargeable electricity and bioelectric potentials produced by muscular movements in flies, Teruo Nonomura, Yoshinori Matsuda, Koji Kakutani, Junji Kimbara, Kazumi Osamura, Shin-ichi Kusakari, Hideyoshi Toyoda, JOURNAL OF ELECTROSTATICS, JOURNAL OF ELECTROSTATICS, 72(1), 1 - 5, Feb. 2014 , Refereed
    Summary:A simple electrostatic apparatus was devised to measure dischargeable electricity and bioelectric potentials produced by flies. The apparatus involved two insulated electrodes, ICW(-) and ICW(+), oppositely charged with equal voltages supplied by two voltage-generators. In the electric field, the flies became net positive by instantaneously discharging their electricity and were attracted to negative surface charges on ICW(-). The tail-lifting movement by the attracted insect was an action creating electric potentials that could cause discharge of ICW(-). The discharge transiently appeared in response to individual movements and was larger when the tail was lifted at higher angles. (C) 2013 Elsevier B.V. All rights reserved.
  • Targeted destruction of fungal structures of Erysiphe trifoliorum on flat leaf surfaces of Marchantia polymorpha, Y. Takikawa, Y. Senga, T. Nonomura, Y. Matsuda, K. Kakutani, H. Toyoda, PLANT BIOLOGY, PLANT BIOLOGY, 16(1), 291 - 295, Jan. 2014 , Refereed
    Summary:In this study, we observed the germination behaviour of airborne conidia from powdery mildews that settle on thalloid surfaces. We inoculated thalli (flat, sheet-like leaf tissues) and gemmae (small, flat, sheet-like leaf tissues that propagate asexually via bud-like structures) of the common liverwort (Marchantia polymorpha) with conidia from tomato powdery mildew (Oidium neolycopersici; KTP-02) and red clover powdery mildew (Erysiphe trifoliorum; KRCP-4N) and examined their germination and subsequent appressorium formation under a high-fidelity digital microscope. Conidial bodies and germ tubes of the inoculated KRCP-4N conidia were destroyed on both the thalli and gemmae. The destruction of these fungal structures was observed only for KRCP-4N conidia inoculated onto M.polymorpha on both leaf surfaces. No differences in destruction of the KRCP-4N fungal structures between thalli and gemmae were observed. At 4h post-inoculation, destruction of the germ tube tip was observed when it reached the gemmae leaf surface. At 6h post-inoculation, the conidial bodies and germ tubes were destroyed. In contrast, KTP-02 conidia were not destroyed and formed normal, well-lobed appressoria on the surface of M.polymorpha gemmae.
  • The isolation and identification of a light-induced protein in alfalfa sprouts and the cloning of its specific promoter, Xin Su, Wei-Zhuo Xu, Xin Liu, Rui-Fang Zhuo, Cai-Yun Wang, Xin Zhang, K. Kakutani, Song You, GENE, GENE, 520(2), 139 - 147, May 2013 , Refereed
    Summary:We used 2D-PAGE to isolate a light-induced protein (AL-A) that is expressed abundantly in light-growth alfalfa sprouts. The seven amino adds of the N-terminal region of the protein were identified, and we searched for the protein in GenBank using the BLAST program. The results of the homology analysis showed that the amino add sequence of the isolated protein is most similar to one from a pea plastocyanin. To identify the protein, we amplified and sequenced the DNA fragment encoding AL-A from genomic alfalfa DNA. We found that the AL-A gene was highly homologous (90%) to the sequences from the pea plastocyanin via multiple alignments, and the deduced protein precursor was predicted to be chloroplast-specific via the ChloroP computer program. The protein was named alfalfa-plastocyanin (AL-P). It was characterized as being a light-inducible protein, and RT-PCR analysis showed that AL-P mRNA transcription only occurred in the leaves of the alfalfa plant and the alfalfa seedlings growth in lighted conditions. PCR was also used to amplify the DNA fragment encoding the AL-P promoter (AL-Pp) from genomic alfalfa DNA. PlantCARE analysis of the promoter sequence indicated that both a typical TATA box and a CAAT box were located in the promoter sequence, and some of the cis-elements that are responsible for light responsiveness were also identified within this promoter region. The AL-P gene promoter fused to the beta-glucuronidase (GUS) reporter gene has been examined for expression in transgenic alfalfa seedlings. Our findings have a potential application in plant genetic engineering; the AL-Pp may be used to drive the expression of heterologous genes in transgenic alfalfa plants. (c) 2013 Elsevier B.V. All rights reserved.
  • Natural Woody Plant, Mallotus japonicus, as an Ecological Partner to Transfer Different Pathotypic Conidia of Oidium neolycopersici to Greenhouse Tomatoes, Teruo Nonomura, Yoshinori Matsuda, Shun Yamashita, Haruhiko Akahoshi, Yoshihiro Takikawa, Koji Kakutani, Hideyoshi Toyoda, PLANT PROTECTION SCIENCE, PLANT PROTECTION SCIENCE, 49, S33 - S40, 2013 , Refereed
    Summary:In our routine surveys for the powdery mildew disease in greenhouse tomatoes, we detected a new pathogen that forms pseudochains consisting of 12 conidia. To identify the original plant that dispersed this pathogen, wild plants infected with powdery mildew were monitored. The pathogen on Japanese mallotus, Mallotus japonicus, produced a similar type of pseudochain, and conidia were infectious to tomatoes. Inversely, the conidia on the tomato leaves infected M. japonicus. Infectivity assays and internal transcribed spacer (ITS)-based phylogenetic analyses indicated that the two pathogens on the tomato and M. japonicus were identical. These results suggest that the conidia on M. japonicus can be transmitted to greenhouse tomatoes. This work documents the ecological transmission of conidia between wild plants and greenhouse tomatoes.
  • An electric field strongly deters whiteflies from entering window-open greenhouses in an electrostatic insect exclusion strategy, Teruo Nonomura, Yoshinori Matsuda, Koji Kakutani, Junji Kimbara, Kazumi Osamura, Shin-ichi Kusakari, Hideyoshi Toyoda, EUROPEAN JOURNAL OF PLANT PATHOLOGY, EUROPEAN JOURNAL OF PLANT PATHOLOGY, 134(4), 661 - 670, Dec. 2012 , Refereed
    Summary:Dual functions (insect repelling and capturing) of a single-charged dipolar electric field screen were evaluated to successfully exclude whiteflies from a window-open greenhouse. The screen consisted of three parts: 1) insulated conductor wires (ICWs) arrayed in parallel at 5 mm intervals, 2) two earthed stainless nets placed within 3 mm of both sides of the ICW layer, and 3) a voltage generator for the negatively charged ICWs. The screen formed two electric fields between the ICW-layer and the ICW-side surface of the earthed net and between the ICWs. At negative charging of 1.5-2.5 kV, all whiteflies reaching the outer surface of the screen net avoided entering the electric field and flew away from the screen. This avoidance was disturbed by 3 m s(-1) wind, as the insects were compulsorily blown inside. However, almost all whiteflies (99.4 %) were captured with the ICW. These results indicate that the insect-capturing function is effective to complement a failure to repel. A greenhouse assay was conducted in the screen-attached and non-screened parts in which a greenhouse was divided with a partition. During the 3-month operation, the screen was durable and functional for excluding pests, and better air ventilation changed the climate conditions in the greenhouse. Thus, the present study demonstrated that our electric field screen can provide an airy condition for tomatoes in a window-open greenhouse and successfully exclude whiteflies using dual screen functions.
  • An oppositely charged insect exclusion screen with gap-free multiple electric fields, Yoshinori Matsuda, Koji Kakutani, Teruo Nonomura, Junji Kimbara, Shin-ichi Kusakari, Kazumi Osamura, Hideyoshi Toyoda, JOURNAL OF APPLIED PHYSICS, JOURNAL OF APPLIED PHYSICS, 112(11), 116103, Dec. 2012 , Refereed
    Summary:An electric field screen was constructed to examine insect attraction mechanisms in multiple electric fields generated inside the screen. The screen consisted of two parallel insulated conductor wires (ICWs) charged with equal but opposite voltages and two separate grounded nets connected to each other and placed on each side of the ICW layer. Insects released inside the fields were charged either positively or negatively as a result of electricity flow from or to the insect, respectively. The force generated between the charged insects and opposite ICW charges was sufficient to capture all insects. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4767635]
  • An electric field screen prevents captured insects from escaping by depriving bioelectricity generated through insect movements, Koji Kakutani, Yoshinori Matsuda, Kayo Haneda, Dai Sekoguchi, Teruo Nonomura, Junji Kimbara, Kazumi Osamura, Shin-ichi Kusakari, Hideyoshi Toyoda, JOURNAL OF ELECTROSTATICS, JOURNAL OF ELECTROSTATICS, 70(2), 207 - 211, Apr. 2012 , Refereed
    Summary:An insulated conductor wire (ICW) paralleled with an earthed net was used to observe movements by vinegar flies in relation to their electricity release. ICW was negatively charged to create a positive charge on the net. At particular voltages, flies were attracted to ICW. This attraction was triggered by the deprivation of the insect negative charge with the net. Eventually the insects became net positive and were drawn to the ICW negative charge. The attracted insects generated bioelectricity through skeletal muscular movements. However, the electricity produced was depleted by the net without neutralizing their positive charge in the insect body. (c) 2012 Elsevier B.V. All rights reserved.
  • Insects are electrified in an electric field by deprivation of their negative charge, K. Kakutani, Y. Matsuda, K. Haneda, T. Nonomura, J. Kimbara, S. Kusakari, K. Osamura, H. Toyoda, ANNALS OF APPLIED BIOLOGY, ANNALS OF APPLIED BIOLOGY, 160(3), 250 - 259, 2012 , Refereed
    Summary:An electric field screen (EF-screen) is a physical device for excluding pest insects from greenhouses and warehouses to protect crops during their production and storage periods. In this study, a simple version of the EF-screen, an insulated conductor iron wire (ICW) paralleled to an earthed net, was constructed to effectively observe the attraction of test insects in relation to their electricity release. The ICW was negatively charged to dielectrically polarise the insulator sleeve of the ICW: negatively on the outer surface and positively on the inner conductor wire surface of the sleeve. The negative surface charge of the ICW caused an electrostatic induction in the earthed net and a resultant positive charge at the ICW-side surface of the net. An electric field formed between the ICW (negative pole) and earthed net (positive pole). Insects were attracted to the ICW when they were placed onto the earthed net. A vital step for the attraction was the creation of a transient bioelectric discharge from an insect. During this discharge, an electric charge of the insect was transferred to the earthed net. Eventually, the insect became net positive and was then attracted to the ICW. The magnitude of the current increased in direct proportion to the increase in voltage applied to the ICW, and the attraction force was directly proportional to the increase in the electric current. Larger voltages were necessary to attract much larger insects because larger insects were stronger and therefore more able to escape from the ICW attraction. Similar results were obtained for a wide range of pest insects belonging to different taxonomic groups (8 orders and 15 families). This study demonstrated that transient bioelectric discharge is common in insects and can be utilised to create an electrostatic force capable of moving insects in a generated electric field.
  • Insects are electrified in an electric field by deprivation of their negative charge, K. Kakutani, Y. Matsuda, K. Haneda, T. Nonomura, J. Kimbara, S. Kusakari, K. Osamura, H. Toyoda, ANNALS OF APPLIED BIOLOGY, ANNALS OF APPLIED BIOLOGY, 160(3), 250 - 259, 2012 , Refereed
    Summary:An electric field screen (EF-screen) is a physical device for excluding pest insects from greenhouses and warehouses to protect crops during their production and storage periods. In this study, a simple version of the EF-screen, an insulated conductor iron wire (ICW) paralleled to an earthed net, was constructed to effectively observe the attraction of test insects in relation to their electricity release. The ICW was negatively charged to dielectrically polarise the insulator sleeve of the ICW: negatively on the outer surface and positively on the inner conductor wire surface of the sleeve. The negative surface charge of the ICW caused an electrostatic induction in the earthed net and a resultant positive charge at the ICW-side surface of the net. An electric field formed between the ICW (negative pole) and earthed net (positive pole). Insects were attracted to the ICW when they were placed onto the earthed net. A vital step for the attraction was the creation of a transient bioelectric discharge from an insect. During this discharge, an electric charge of the insect was transferred to the earthed net. Eventually, the insect became net positive and was then attracted to the ICW. The magnitude of the current increased in direct proportion to the increase in voltage applied to the ICW, and the attraction force was directly proportional to the increase in the electric current. Larger voltages were necessary to attract much larger insects because larger insects were stronger and therefore more able to escape from the ICW attraction. Similar results were obtained for a wide range of pest insects belonging to different taxonomic groups (8 orders and 15 families). This study demonstrated that transient bioelectric discharge is common in insects and can be utilised to create an electrostatic force capable of moving insects in a generated electric field.
  • An Argon Excimer Lamp as a Source of Vacuum Ultraviolet Light for Inducing Mutations that Lead to Morphological Changes in Plants, Yoshihiro Takikawa, Koji Kakutani, Yasuo Takigawa, JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN, JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN, 80(9), 95002 - kakutanikoji, Sep. 2011 , Refereed
  • Conidia of Erysiphe trifoliorum attempt penetration twice during a two-step germination process on non-host barley leaves and an artificial hydrophobic surface, Yoshihiro Takikawa, Koji Kakutani, Teruo Nonomura, Yoshinori Matsuda, Hideyoshi Toyoda, MYCOSCIENCE, MYCOSCIENCE, 52(3), 204 - 209, May 2011 , Refereed
    Summary:In the present study, using a high-fidelity digital microscope, we observed the sequence of appressorial development on the germ tubes of a powdery mildew fungus isolated from red clover leaves. Based on its morphological characteristics and rDNA internal transcribed spacer (ITS) sequences, the fungus was identified as Erysiphe trifoliorum, and one of its isolates, designated as KRCP-4N, was used in this work. The conidial germination of isolate KRCP-4N was studied on host (red clover) and non-host (barley) leaves, as well as on an artificial hydrophobic membrane (Parafilm). More than 90% of conidia germinated synchronously and developed dichotomous appressoria (symmetrical double-headed appressoria) on all substrata used. On host leaves, all appressorium-forming conidia developed hyphae (colony-forming hyphae) from conidial bodies without extending germ tubes from the tips of the appressoria. On non-host leaves and on Parafilm-covered glass slides, however, all conidia extended germ tubes from one side of dichotomous appressoria (two-step germination). In addition to the dichotomous appressoria, we detected a few conidia that produced hooked appressoria and extended germ tubes from the tip of the appressorium. Penetration attempts by KRCP-4N conidia on barley leaves were impeded by papillae formed at penetration sites beneath these two types of appressorium. From these results, we conclude that the "two-step germination" of E. trifoliorum KRCP-4N conidia is the result of an unsuccessful penetration attempt, causing diversity in appressorial shape.
  • Conidia of the tomato powdery mildew Oidium neolycopersici initiate germ tubes at a predetermined site, Yoshihiro Takikawa, Ling Xu, Koji Kakutani, Teruo Nonomura, Takeshi Sameshima, Yoshinori Matsuda, Hideyoshi Toyoda, MYCOSCIENCE, MYCOSCIENCE, 52(3), 198 - 203, May 2011 , Refereed
    Summary:The emergence of germ tubes from the conidia of powdery mildew fungi is the first morphological event of the infection process, preceding appressoria formation, peg penetration and primary haustoria formation. Germination patterns of the conidia are specific in powdery mildew fungi and therefore considered useful for identification. In the present study, we examined conidial germination of the tomato powdery mildew Oidium neolycopersici KTP-01 in order to clarify whether germ tube emergence site in KTP-01 conidia is determined by the first contact of the conidia to leaves (as found for the conidia of barley powdery mildew), or alternatively is predetermined and is unrelated to contact stimulus. Highly germinative conidia of KTP-01 were collected from conidial pseudochains on conidiophores in colonies on tomato leaves using two methods involving an electrostatic spore attractor and a blower. In the electrostatic spore attraction method, the conidia were attracted to the electrified insulator probe of the spore collector-this being the first contact stimulus for the conidia. In addition, the blowing method was used as a model of natural infection; pseudochain conidia were transferred to detached leaves by air (1 m/s) from a blower. Thus, landing on the leaves was the first contact for the conidia. Furthermore, conidia were also blown onto an artificial membrane (Parafilm-coated glass slides forming a hydrophobic surface) or solidified agar plates in Petri dishes (hydrophilic surface). Eventually, almost all conidia on the probe and on tomato leaves or artificial hydrophobic and hydrophilic surfaces synchronously germinated within 6 h of incubation, indicating that the first contact of the conidia with any of the aforementioned substrata was an effective germination induction signal. Germ tube emergence sites were exclusively subterminal on the conidia. Moreover, the germ tubes emerged without any relation to the sites touched first on the conidia. Thus, the present study strongly indicates that conidia of O. neolycopersici produce germ tubes at a predetermined site.
  • A newly devised electric field screen for avoidance and capture of cigarette beetles and vinegar flies, Yoshinori Matsuda, Teruo Nonomura, Koji Kakutani, Yoshihiro Takikawa, Junji Kimbara, Yoshihiro Kasaishi, Kazumi Osamura, Shin-ichi Kusakari, Hideyoshi Toyoda, CROP PROTECTION, CROP PROTECTION, 30(2), 155 - 162, Feb. 2011 , Refereed
    Summary:A bifunctional electric field screen was proposed to physically exclude insect pests from warehouses. The screen consists of insulated iron wires (ICW) arranged in parallel and two earthed conductor nets placed on both sides of the ICW. A negative charge (0.1-8.0 kV) was applied to the insulated wires with a voltage generator to polarize an insulator sleeve used to cover the wire, negatively on the outer surface and positively on the inner conductor wire surface of the sleeve. The negative surface charge of the ICW caused an electrostatic induction in the earthed nets and an opposite charge on the net surfaces facing the ICW. An electric field formed in a space between the ICW and the earthed net, and the field strength increased in direct proportion to increasing voltages applied to the ICW. Adults of the test insects (cigarette beetle (Lasioderma serricorne) and vinegar fly (Drosophila melanogaster)) reaching the outer surface of the earthed net were deterred from entering the inside of the charged screen, whereas all insects immediately passed through the screen when the ICW was not charged. This avoidance was directly proportional to the increase in the voltage. In addition, the capability of the screen to capture insects that enter inside the screen was proven by introducing insects into the space between the ICW and the earthed net. Strong capture was observed when the ICW was negatively charged with more than 4.1 kV, under which conditions a short-term electric current (peaking at 0.3-0.6 mu A, for 3 min) occurred transiently. This electric current was due to the release of electricity from the insects, giving a net overall positive charge to the insects, which therefore were attracted more strongly to the negatively charged ICW. A test using an attractant-set chamber showed that the insects were completely prevented from passing through the charged screen, in contrast to a rapid transfer of all insects when the screen was not charged. Thus, the present results show that the described screen is a promising physical tool for controlling insect pests in warehouses. (C) 2010 Elsevier Ltd. All rights reserved.
  • Polymorphic change of appressoria by the tomato powdery mildew Oidium neolycopersici on host tomato leaves reflects multiple unsuccessful penetration attempts, Teruo Nonomura, Ayae Nishitomi, Yoshinori Matsuda, Chiyomi Soma, Ling Xu, Koji Kakutani, Yoshihiro Takikawa, Hideyoshi Toyoda, FUNGAL BIOLOGY, FUNGAL BIOLOGY, 114(11-12), 917 - 928, Nov. 2010 , Refereed
    Summary:The appressorial shapes of the powdery mildews are an important clue to the taxonomy of the powdery mildew fungi, but the conidia of the tomato powdery mildew Oidium neolycopersici KTP-01 develop non-lobed, nipple-shaped, and moderately lobed or multilobed appressoria on the same leaves. To remove this ambiguity, we performed consecutive observations of sequential appressorial development of KTP-01 conidia with a high-fidelity digital microscope. Highly germinative conidia of KTP-01, collected from conidial pseudo-chains formed on the tomato leaves, were inoculated into host tomato and nonhost barley leaves or an artificial hydrophobic membrane (Parafilm). Events from germination initiation to appressorium formation were synchronous in all conidia on all materials used for inoculation, but post-appressorial behaviors varied among the materials. Appressoria on the membrane-stuck glass slide formed several projections at different portions of the appressoria to repeat unsuccessful penetration attempts. Similar unsuccessful penetration behavior by KTP-01 conidia was observed in the inoculations into leaves of barley plants, wild tomato species Lycopersicon peruvianum LA2172 (carrying the Ol-4 gene for powdery mildew resistance), and a susceptible host tomato (Lycopersicon esculentum) that had been inoculated with the barley powdery mildew (Blumeria graminis f. sp. hordei, race 1) conidia. On the barley leaves, all penetrations of KTP-01 were impeded by the papillae formed beneath the sites of the appressorial projections. On both the wild tomato and the race 1-inoculated cultivated tomato plants, KTP-01 conidia were prevented from forming functional haustoria by hypersensitive epidermal cell death; this hypersensitive reaction involved the Ol-4 gene in the wild tomato plants or the 'induced resistance' acquired by the nonpathogenic conidia previously inoculated into the cultivated tomato plants. All these KTP-01 conidia produced several projections on the appressoria during the repeated unsuccessful penetration attempts and eventually exhibited multilobed appressoria. On the host tomato leaves inoculated singly with KTP-01 conidia, fewer than 20 % of the conidia located appressoria on the central part of target epidermal cells and succeeded in forming functional haustoria at the first penetration attempt without forming an appressorial projection. These conidia exhibited non-lobed appressoria. The remaining conidia, however, whose appressoria were located on/near the border of the target epidermal cells, were more likely to fail to penetrate at the first penetration, and then to develop additional projections for subsequent penetrations. Most conidia succeeded in forming functional haustoria at the second to fourth penetration attempts, but a few conidia failed to produce haustoria at all attempted penetrations. Eventually, the conidia that succeeded at the second penetration possessed a single appressorial projection (exhibiting the nipple-shaped appressoria), whereas the remaining conidia exhibited moderately lobed appressoria with two to four appressorial projections and multilobed appressoria, with more projections. Thus, the present study revealed that the basic shape of appressoria of KTP-01 was the non-lobed type, and that polymorphic changes of the appressoria occurred as a result of successive production of projections during repeated unsuccessful penetration attempts. (C) 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
  • Tryptophan synthesis block-associated sugar response, a physiological marker for rapid selection of Arabidopsis thaliana transformants, marked with feedback-inhibition-insensitive anthranilate synthase gene, T. Nonomura, S. Komaki, L. Xu, N. Moriura, H. Ioroi, S. Takashima, K. Kakutani, Y. Takikawa, Y. Matsuda, H. Toyoda, PLANT BREEDING, PLANT BREEDING, 128(3), 282 - 289, Jun. 2009 , Refereed
    Summary:The feedback-insensitive anthranilate synthase (AS) gene was used as a selection marker for transformants of Arabidopsis thaliana. The mutant gene (mAS1-2) was constructed by substituting nucleotide at the effector-binding site of the intrinsic AS gene via PCR-mediated site-directed mutagenesis and flanked with the myrosinase promoter pyk10 to drive its expression during initial root elongation. This inducible gene cassette was first introduced into Agrobacterium tumefaciens and then delivered into A. thaliana by floral-dip inoculation. 5-methyltryptophan (5-MT) inhibited AS and suppressed seedling growth of wild type plants as a result of tryptophan starvation. With the addition of sucrose (10 mg/ml), 5-MT inhibited cotyledon opening and caused anthocyanin to accumulate in juvenile seedlings. The present mutant reversed the tryptophan starvation caused by 5-MT and blocked subsequent sugar responses. The sugar responses were detected in non-transformed plants grown on a selection medium containing 10 mg/ml of sucrose and 10 mu g/ml of 5-MT after 3 days of incubation. Thus, true transformants could be selected after a short incubation, compared to the conventional kanamycin-selection method that did not eliminate all non-transformed plants.
  • Collection of highly germinative pseudochain conidia of Oidium neolycopersici from conidiophores by electrostatic attraction, Teruo Nonomura, Yoshinori Matsuda, Ling Xu, Koji Kakutani, Yoshihiro Takikawa, Hideyoshi Toyoda, MYCOLOGICAL RESEARCH, MYCOLOGICAL RESEARCH, 113(3), 364 - 372, Mar. 2009 , Refereed
    Summary:A population of simultaneously germinating conidia is an ideal inoculum of the powdery mildew pathogen, Oidium neolycopersici. In conditions of no or low wind velocity, O. neolycopersici successively stacks mature conidia on conidiophores in a chain formation (pseudochain), without releasing the precedent mature conidia. These pseudochain conidia represent a perfect inoculum, in which all conidia used for inoculation germinate simultaneously. However, we found that conidia must be collected before they fall to the leaf surface, because the germination rate was lower among conidia deposited on the leaf surface. We used an electrostatic spore collector to collect the pseudochain conidia, and their high germination rate was not affected by this treatment. The spore collector consisted of an electrified insulator probe, which created an electrostatic field around its pointed tip, and attracted conidia within its electric field. The attractive force created by the probe tip was directly proportional to voltage, and was inversely proportional to the distance between the tip and a target colony on a leaf. Pseudochain conidia were successfully collected by bringing the electrified probe tip close to target colonies on leaves. In this way, conidia were collected from colonies at 3-d intervals. This effectively collected all conidia from conidiophores before they dropped to the leaf surface. A high germination rate was observed among conidia attracted to the probe tip (95.5 +/- 0.6 %). Conidia were easily suspended in water with added surfactant, and retained their germination ability. These conidia were infective and produced conidia in pseudochains on conidiophores after inoculation. The electrostatic spore collection method can be used to collect conidia as they form on conidiophores, thus obtaining an inoculum population in which all of the conidia germinate simultaneously. (c) 2008 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
  • Trichome exudates of Lycopersicon pennellii form a chemical barrier to suppress leaf-surface germination of Oidium neolycopersici conidia, Teruo Nonomura, Ling Xu, Mizuki Wada, Shuzou Kawamura, Takemasa Miyajima, Ayae Nishitomi, Koji Kakutani, Yoshihiro Takikawa, Yoshinori Matsuda, Hideyoshi Toyoda, PLANT SCIENCE, PLANT SCIENCE, 176(1), 31 - 37, Jan. 2009 , Refereed
    Summary:Secretion by glandular trichomes is thought to be a strategy to retain moisture water on leaves under drought conditions. In the wild tomato species Lycopersicon pennellii exudates accumulate at the apical terminal of the trichome, forming a bulbous head. During a 30 s mist treatment, the exudates condensed moisture water to form water drops on the trichome heads, and water drops were absorbed through trichomes by the plants. However, a longer mist treatment (40 s) caused the drops on all trichome heads to drop down to the leaf surface. Water drops containing the exudates were amphiphilic and spread over the leaf surface in a thin layer. This is an additional mechanism of the glandular trichomes for covering leaves with the exudates as a chemical boundary layer. Exudates of type IV glandular trichomes of L. pennellii showed antifungal activity against conidia of the tomato powdery mildew pathogen Oidium neolycopersici. The exudates covering the leaves completely suppressed germination of inoculated conidia, which prevented subsequent infection events such as formation of appressoria and haustoria. On non-misted leaves almost all conidia germinated and formed appressoria, resulting in successful infection. L. pennellii is susceptible to tomato powdery mildew, but the mechanisms for condensing mist water by type IV trichome-derived exudates can contribute to suppressing foliar infection by the powdery mildew pathogen. Crown Copyright (C) 2008 Published by Elsevier Ireland Ltd. All rights reserved.
  • Physical control of powdery mildew (Oidium neolycopersici) on tomato leaves by exposure to corona discharge, T. Nonomura, Y. Matsuda, K. Kakutani, Y. Takikawa, H. Toyoda, CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE, CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE, 30(4), 517 - 524, Oct. 2008 , Refereed
    Summary:We devised a cylindrical electrostatic discharge generator to physically eradicate tomato powdery mildew colonizing tomato leaves. The generator consists of a copper needle with a pointed tip, an insulating acrylic cylinder, and an electrostatic voltage generator. The needle is insulated with a vinyl sleeve, except for the pointed tip, and is coaxially fixed in the cylinder and connected to the voltage generator. The needle is negatively charged, and the treated plant is earthed. In initial tests, a corona, characterized by a blue glow, formed at the needle tip as the probe was brought closer to the leaf surface. The distance at which this Occurred increased from 16 to 50 mm as the voltage was increased from 5 to 30 kV. If the probe was brought too close to the leaf surface, an arc discharge Occurred that caused injury to the leaf. Powdery mildew colonies were destroyed by 2-second exposures at probe distances intermediate to where corona discharge was initiated and where arcing Occurred. A probe distance of 25 mm and 30 kV for a 2-second burst was selected to further test the efficacy of the probe for controlling powdery mildew in a greenhouse environment. Tomato plants were grown hydroponically in two open-window greenhouses under a first-truss cropping system. Colonies appeared on tomato leaves 10 to 14 days after transplanting. During the following 2 weeks, these colonies produced abundant progeny conidia that secondarily infected neighboring plants. Corona discharge treatment in one greenhouse, at the stage when colonies first became visible, completely Suppressed the spread of the disease compared with a non-treated greenhouse in which disease spread rapidly. The present discharge generator is portable and easy to operate on-site as a part of routine care of hydroponically Cultured tomatoes in greenhouses and provides a non-chemical method to control powdery mildew disease.
  • Antioxidant constituents in the dayflower (Commelina communis L.) and their alpha-glucosidase-inhibitory activity, Makio Shibano, Koji Kakutani, Masahiko Taniguchi, Masahide Yasuda, Kimiye Baba, JOURNAL OF NATURAL MEDICINES, JOURNAL OF NATURAL MEDICINES, 62(3), 349 - 353, Jul. 2008 , Refereed
    Summary:The dayflower, Commelina communis L., contains 1-deoxynojirimycin (DNJ) and (2R,3R,4R,5R)2,5-bis(hydroxymethyl)-3,4-dihydroxypyrrolidine (DMDP), potent alpha-glucosidase inhibitors. The extracts and powder of this herb are important food materials for prophylaxis against type 2 diabetes. Eleven flavonoid glycosides as antioxidants, isoquercitrin, isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-beta-D-glucoside, glucoluteolin, chrysoriol-7-O-beta-D-glucoside, orientin, vitexin, isoorientin, isovitexin, swertisin, and flavocommelin, were identified from the aerial parts of C. communis. Their antioxidant activities were measured using in vitro assays employing the 1,1-diphenyl-2-picrylhydrazyl radical- and superoxide radical-scavenging assays. The results showed that glucoluteolin, orientin, isoorientin, and isoquercitrin are the predominant antioxidants in this herb. Moreover, isoquercitrin, isorhamnetine-3-O-rutinoside, vitexin, and swertisin inhibited the activity of alpha-glucosidase from rat intestine.
  • Identification of DNA polymorphisms in Angelica acutiloba, Jun Murata, Hiroyuki Oki, Koji Kakutani, Takashi Hashimoto, PLANT BIOTECHNOLOGY, PLANT BIOTECHNOLOGY, 25(2), 157 - 163, 2008 , Refereed
    Summary:Angelica acutiloba and related species have been known for their analgesic and sedative effects, and are widely used as the key ingredient in Japanese traditional Kampo medicine. Among various varieties, Angelica acutiloba var. acutiloba has long been grown in the Obuka area of Nara prefecture, Japan, and has been evaluated as an excellent variety. Recent cultivation of this and other varieties of Angelica in various regions of East Asia prompted us to identify DNA polymorphisms that distinguish the acutiloba variety from others. Random Amplified Polymorphic DNA analysis and sequencing selected genomic regions among Angelica plants of different origins identified several nucleotide changes among the varieties tested, which enabled us to distinguish the acutiloba variety from others. We also report an improved protocol for genomic DNA extraction from dried root samples of Angelica.
  • Genetic variation of sweet potato weevils, Cylas formicarius (Fabricius) (Coleoptera : Brentidae), in main infested areas in the world based upon the internal transcribed spacer-1 (ITS-1) region, Kiyohisa Kawamura, Tuyosi Sugimoto, Koji Kakutani, Yoshinori Matsuda, Hideyoshi Toyoda, APPLIED ENTOMOLOGY AND ZOOLOGY, APPLIED ENTOMOLOGY AND ZOOLOGY, 42(1), 89 - 96, Feb. 2007 , Refereed
    Summary:Genetic variations of the internal transcribed spacer-1 region in sweet potato weevil, Cylasfornficarius, were examined from 27 collection sites in the main infested areas in the world, except Africa, in this study. The ITS-1 lengths of 36 weevils tested in this study ranged from 557 to 587 bp, and were conspicuously longer in weevils from India. The genetic distances between weevils from India and from the other areas tested were larger than among weevils from the latter areas. The phylogenetic tree based on ITS-1 sequences consisted of two main clades of India and East Asia consisting of Northeast Asia and Southeast Asia subclades. The former subclade was also divided into three minor subclades. Weevils from Georgia and Hawaii (USA), and St. Kitts (the West Indies) belonged to the same subclade as those from Guangdong (China) and Hanoi (Vietnam). Also, weevils from the Ogasawara Islands (Japan) belonged to the same subclade. On the other hand, weevils from the Southwest Islands (Japan) belonged to the same subelade as those from Taiwan. When referring to human historical events, C. formicarius would have gradually spread in southern Asia due to its limited dispersal ability since originating in the Indian subcontinent 90 million years ago. However, after becoming associated with sweet potato, especially after the nineteenth century, local populations with fairly different genetic properties from Indian weevils would have been rapidly spread by human transportation of infested sweet potato throughout the world.
  • A new spore precipitator with polarized dielectric insulators for physical control of tomato powdery mildew, Yoshinori Matsuda, Hiroki Ikeda, Nobuyuki Moriura, Norio Tanaka, Kunihiko Shimizu, Wataru Oichi, Teruo Nonomura, Koji Kakutani, Shin-ichi Kusakari, Katsuhide Higashi, Hideyoshi Toyoda, PHYTOPATHOLOGY, PHYTOPATHOLOGY, 96(9), 967 - 974, Sep. 2006 , Refereed
    Summary:In an attempt to physically protect greenhouse tomato plants from the powdery mildew fungus Oidium neolycopersici, we developed a new electrostatic spore precipitator in which a copper wire conductor is linked to an electrostatic generator and covered with a transparent acrylic cylinder (insulator). The conductor was negatively charged by the generator, and the electrostatic field created by the conductor was used to dielectrically polarize the insulator cylinder. The dielectrically polarized cylinder also produced an electrostatic force without a spark discharge. This force was directly proportional to the potential applied to the conductor and was used to attract conidia of the pathogen. The efficacy of this spore precipitator in protecting hydroponically cultured tomato plants from powdery mildew was evaluated in the greenhouse. The hydroponic culture troughs were covered with a cubic frame installed with the spore precipitator, and the disease progress on precipitator-guarded and unguarded seedlings was traced after the conidia were disseminated mechanically from inoculum on tomato plants. Seedlings in the guarded troughs remained uninfected during the entire experiment, in spite of rapid spread of the disease to all leaves of the unguarded seedlings.
  • An apparatus for collecting total conidia of Blumeria graminis f.sp hordei from leaf colonies using electrostatic attraction, N. Moriura, Y. Matsuda, W. Oichi, S. Nakashima, T. Hirai, T. Nonomura, K. Kakutani, S. Kusakari, K. Higashi, H. Toyoda, PLANT PATHOLOGY, PLANT PATHOLOGY, 55(3), 367 - 374, Jun. 2006 , Refereed
    Summary:Conidia from living conidiophores of barley powdery mildew (Blumeria graminis f.sp. hordei) on host leaves were collected consecutively using an electrostatic spore collector. The collector consisted of an electrical conductor plate linked to an electrostatic voltage generator and insulator plates placed abreast on a timed conveyer. The conductor plate was negatively charged by the potential supplied from the voltage generator. The negatively charged conductor plate caused dielectric polarization of the insulator plate, and the surface charge on the insulator plate attracted mature conidia abstricted from conidiophores on colonies growing on leaves placed 2 cm from the insulator plate. The surface charge on the insulator plate was proportional to the voltage applied to the conductor plate. Under optimized conditions, abstricted conidia were attracted to the electrostatically activated insulator plates without any detriment to their survival. During a colony's life span of c. 460 h, conidia were released throughout the day and c. 12 x 10(4) conidia were collected during the lifetime of the colony. This is the first report on the direct quantification of progeny conidia produced by powdery mildew infecting host leaves.
  • Consecutive monitoring of lifelong production of conidia by individual conidiophores of Blumeria graminis f. sp hordei on barley leaves by digital microscopic techniques with electrostatic micromanipulation, N Moriura, Y Matsuda, W Oichi, S Nakashima, T Hirai, T Sameshima, T Nonomura, K Kakutani, S Kusakari, K Higashi, H Toyoda, MYCOLOGICAL RESEARCH, MYCOLOGICAL RESEARCH, 110(1), 18 - 27, Jan. 2006 , Refereed
    Summary:Conidial formation and secession by living conidiophores of Blumeria graminis f. sp. hordei on barley leaves were consecutively monitored using a high-fidelity digital microscopic technique combined with electrostatic micromanipulation to trap the released conidia. Conidial chains formed on conidiophores through a series of septum-mediated division and growth of generative cells. Apical conidial cells on the conidiophores were abstricted after the conidial chains developed ten conidial cells. The conidia were electrically conductive, and a positive charge was induced in the cells by a negatively polarized insulator probe (ebonite). The electrostatic force between the conidia and the insulator was used to attract the abstricted conidia. from the conidiophores on leaves. This conidium movement from the targeted conidiophore to the rod was directly viewed under the digital microscope, and the length of the interval between conidial septation and secession, the total number of the conidia produced by a single conidiophore, and the modes of conidiogenesis were clarified. During the stage of conidial secession, the generative cells pushed new conidial cells upwards by repeated division and growth. The successive release of two apical conidia was synchronized with the successive septation and growth of a generative cell. The release ceased after 4-5 conidia were released without division and growth of the generative cell. Thus, the life of an individual conidiophore (from the erection of the conidiophore to the release of the final conidium) was shown to be 107 h and to produce an average of 33 conidia. To our knowledge, this is the first report on the direct estimation of life-long conidial production by a powdery mildew on host leaves. (c) 2005 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
  • Identification of individual powdery mildew fungi infecting leaves and direct detection of gene expression by single conidium polymerase chain reaction, Y Matsuda, T Sameshima, N Moriura, K Inoue, T Nonomura, K Kakutani, H Nishimura, S Kusakari, S Takamatsu, H Toyoda, PHYTOPATHOLOGY, PHYTOPATHOLOGY, 95(10), 1137 - 1143, Oct. 2005 , Refereed
    Summary:Greenhouse-grown tomato seedlings were inoculated naturally with two genera of powdery mildew conidia forming appressorial germ tubes that could not be differentiated by length alone. For direct identification, single germinated conidia were removed from leaves by means of a glass pipette linked to the manipulator of a high-fidelity digital microscope. This microscope enabled in vivo observation of the fungi without leaf decoloration or fungal staining. The isolated conidia were subjected to PCR amplification of the 5.8S rDNA and its adjacent internal transcribed spacer sequences followed by nested PCR to attain sensitivity high enough to amplify target nucleotide sequences (PCR/nested PCR). Target sequences from the conidia were completely coincident with those of the pathogen Oidium neolycopersici or Erysiphe trifolii (syn. Microsphaera trijblii), which is nonpathogenic on tomato. Using RT-PCR/nested PCR or multiplex RT-PCR/nested PCR, it was possible to amplify transcripts expressed in single conidia. Conidia at pre- and postgermination stages were removed individually from tomato leaves, and two powdery mildew genes were monitored. The results indicated that the P-tubulin homolog TUB2-ol was expressed at pre- and postgermination stages and the cutinase homolog CUT1-ol was only expressed postgermination. Combining digital microscopic micromanipulation and two-step PCR amplification is thus useful for investigation of individual propagules on the surface of plants.
  • Powdery mildew pathogens can suppress the chitinase gene expression induced in detached inner epidermis of barley coleoptile, K Fujita, Y Matsuda, M Wada, Y Hirai, K Mori, N Moriura, T Nonomura, K Kakutani, H Toyoda, PLANT CELL REPORTS, PLANT CELL REPORTS, 23(7), 504 - 511, Dec. 2004 , Refereed
    Summary:Two-step PCR (RT-PCR and nested PCR) was used to detect gene expression in powdery mildew pathogen-infected cells of detached inner epidermis of barley coleoptiles. Cellular contents of infected cells were microscopically suctioned with a micropipette and subjected to PCR. Triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase genes involved in the glycolytic pathway and a stimulus-induced endochitinase gene were targeted, and their expression was determined by detecting cDNAs derived from spliced transcripts. The two gycolysis-related genes were constantly expressed in the tissue irrespective of pathogen inoculation. In contrast, chitinase gene expression was induced in non-infected inner epidermis after detachment. After inoculation, this expression was selectively suppressed in pathogen-invaded cells, in spite of continuous expression in non-invaded cells of the same epidermis. Thus, the present method enabled us to directly analyze transcripts in individual cells at the infection site and assess the capability of the pathogen to regulate host gene expression.
  • Consecutive monitoring for conidiogenesis by Oidium neolycopersici on tomato leaves with a high-fidelity digital microscope, Wataru Oichi, Yoshinori Matsuda, Takeshi Sameshima, Teruo Nonomura, Koji Kakutani, Hiroaki Nishimura, Shin-Ichi Kusakari, Hideyoshi Toyoda, Journal of General Plant Pathology, Journal of General Plant Pathology, 70(6), 329 - 332, Dec. 2004 , Refereed
    Summary:Conidiogenesis by Oidium neolycopersici KTP-01 on tomato leaves was vitally monitored with a high-fidelity digital microscope. Conidiophores were initially formed 3 days after inoculation and then elongated to a maximum length within at least 12 h. The apical part was split into two cells after two successive septations, accompanied by apical expansion. These cells subsequently developed into primary and secondary conidia. An additional septation at the stem portion of the conidiophores produced a generative and a foot cell. Subsequent conidiation occurred during repeated cycles of splitting of the generative cell, maturation of the apical cell into a conidium, and abstriction of the conidium. To our knowledge, this report is the first on the developmental process of conidiogenesis by powdery mildew on host leaves as revealed with the digital microscope.
  • RT-PCR amplification of mRNAs in nuclei or cytosol of single cells of tomato callus after micropipette extraction, M Wada, Y Matsuda, K Fujita, A Nanjo, M Nishimura, T Nonomura, K Kakutani, H Toyoda, PLANT CELL TISSUE AND ORGAN CULTURE, PLANT CELL TISSUE AND ORGAN CULTURE, 79(1), 109 - 114, Oct. 2004 , Refereed
    Summary:RT-PCR was used to detect gene expression in situ in single selected cells of tomato callus aggregates. The cytoplasm from one cell was removed with a micropipette viewed under a light microscope and used directly for RT-PCR, followed by nested PCR. This method to remove cytosolic contents prevented the introduction of genomic DNA into the RT-PCR, and only intron-spliced products were amplified when intron-containing genes were used as PCR targets. In addition, transcription of the intron-free gene was possibly detected by simultaneously tracing the intron-containing and intron-free genes using mixed primers for the targeted genes. The present study indicated that some stimuli-activated genes, such as CHI3 and TLC1-LTR, were constitutively transcribed in tomato callus cells.
  • Cytological events in tomato leaves inoculated with conidia of Blumeria graminis f. sp. hordei and Oidium neolycopersici KTP-01, Takeshi Sameshima, Koichi Kashimoto, Keiko Kida, Yoshinori Matsuda, Teruo Nonomura, Koji Kakutani, Kengo Nakata, Shin-Ichi Kusakari, Hideyoshi Toyoda, Journal of General Plant Pathology, Journal of General Plant Pathology, 70(1), 7 - 10, Feb. 2004 , Refereed
    Summary:Leaves of tomato and barley were inoculated with conidia of Blumeria graminis f. sp. hordei race 1 (R1) or Oidium neolycopersici (KTP-01) to observe cytological responses in search of resistance to powdery mildew. Both conidia formed appressoria at similar rates on tomato or barley leaves, indicating that no resistance was expressed during the prepenetration stage of these fungi. On R1-inoculated tomato leaves, appressoria penetrated the papillae, but subsequent haustorium formation was inhibited by hypersensitive necrosis in the invaded epidermal cells. On the other hand, KTP-01 (pathogenic to tomato leaves) successfully developed functional haustoria in epidermal cells to elongate secondary hyphae, although the hyphal elongation from some conidia was later suppressed by delayed hypersensitive necrosis in some haustorium-harboring epidermal cells. Thus, the present study indicated that the resistance of tomato to powdery mildew fungi was associated with a hypersensitive response in invaded epidermal cells but not the prevention of fungal penetration through host papilla.
  • Rapid detection of phylloplane bacterium Enterobacter cloacae based on chitinase gene transformation and lytic infection by specific bacteriophages, Y Takikawa, H Mori, Y Otsu, Y Matsuda, T Nonomura, K Kakutani, Y Tosa, S Mayama, H Toyoda, JOURNAL OF APPLIED MICROBIOLOGY, JOURNAL OF APPLIED MICROBIOLOGY, 93(6), 1042 - 1050, 2002 , Refereed
    Summary:Aims: To establish a rapid and efficient method for detecting Enterobacter cloacae based on chitinase gene transformation and lytic infection by virulent bacteriophages. Methods and Results: A phylloplane strain of E. cloacae was isolated from tomato leaves and transformed with a chitinase gene. Transformed bacteria were collected from single colonies and infected with newly isolated, virulent bacteriophages in the presence of the chitinase substrate 4-methylumbelliferon (4MU)-(GlcNac)(3). To assay chitinase activity in the lysates, the product 4MU was measured spectrofluorophotometrically or visibly detected under u.v. irradiation. Chitinase gene-transformed bacteria obtained from single colonies could be specifically identified in 30 min by the emission of 4MU fluorescence following lysis caused by phage infection. Conclusions: The chitinase gene was used as a reporter gene to construct a new system for easy and rapid monitoring of transgenic strains of E. cloacae released in the environment, in combination with specific recognition by virulent bacteriophages. Significance and Impact of the Study: The assay is simple, rapid, inexpensive, easy to perform and applicable to other strains. The system can be used for the routine monitoring of bacteria, which is important because of the increased use of transgenic strains of E. cloacae as an antagonistic biological control agent for plant diseases.

Books etc

  • An illustrated Manual of Electric Field Screens-Their Structures and Functions, Koji Kakutani, Joint author,   2019 03
  • Principles and applications of Electric Field Screens, Toyoda H, Y. Matsuda, Kusakari S, Nonomura T, Kakutani K, Takikawa Y, Research Association of Electric Field Screens,   2015
  • 植物工場生産システムと流通技術の最前線, Xu L, Zhu P, Joint author, 植物工場における薬用植物の栽培・生産技術, エヌ・ティー・エス,   2013 04
  • Direct RT-PCR amplification of mature mRNAs in single trichome cells of plant leaves, Y. Matsuda, K. Kakutani, M. Nishimura, M. Wada, T. Nonomura, H. Toyoda, Joint author, Recent Res. Devel. Cell Bio,   2003

Conference Activities & Talks

  • 植物工場に利用できる機能性および薬用植物の栽培・生産技術, 角谷晃司, 最新研究から見る完全制御型植物工場における新しい植栽植物の可能,   2015 08 , 東京
  • A field investigation of the bryophyte distribution in the mountainous campus of Kinki University: Collection and cytological observation of liverworts as a potential source of nutraceuticals, 瀧川 義浩, 角谷 晃司, 松田 克礼, 野々村 照雄, 豊田 秀吉, MOSS 2011, The 14th annual international conference,   2011 09 , MOSS 2011, The 14th annual international conference
  • The flat liverwort thallus surface is the site of interactions with falling airborne fungal spores: Specification of conidial germination by powdery mildew on a thalloid surface, 瀧川 義浩, 松田 克礼, 角谷 晃司, 野々村 照雄, 豊田 秀吉, MOSS 2011, The 14th annual international conference,   2011 09 , MOSS 2011, The 14th annual international conference
  • Leaf surface of the moss is the site for trapping airborne fungal spores as nutritional targets, 瀧川 義浩, 角谷 晃司, 松田 克礼, 野々村 照雄, 豊田 秀吉, MOSS 2011, The 14th annual international conference,   2011 09 , MOSS 2011, The 14th annual international conference
  • Production of wholesome plants by introduction of genes for tumor-specific protein (CD98),, 角谷 晃司, 野々村 照雄, 松田 克礼, 豊田 秀吉, 近畿大学大学院農学研究科, XVII Interntional Botanical Congress,   2005 06 , XVII Interntional Botanical Congress
  • Biological control of powdery mildew and gray mold of tomato by chitinolytic phylloepiphytes ? Biological control of phyllophagous ladybird beetles of chitinolytic bacteria, 豊田 秀吉, 松田 克礼, 藤原圭一, 野々村 照雄, 桜谷保之, 角谷 晃司, 大津康成, 土佐幸雄, 眞山滋志, 平成15 年度日本植物病理学会関西部会(奈良),   2003 10 , 平成15 年度日本植物病理学会関西部会(奈良)
    Summary:本実験では、トマトから葉圏に生息するキチナーゼ生産性細菌を分離し、ニジュウヤホシテントウの生物防除を試みた。まず、最も高いキチン分解活性を示す細菌KPM.012A を中腸ペリトロフィック膜に処理したところ、キチナーゼによる分解が確認された。
  • Biological control of powdery mildew and gray mold of tomato by chitinolytic phylloepiphytes ? Biological control of phyllophagous ladybird beetles by entomopathogenic bacteria, 豊田 秀吉, 藤原圭一, 松田 克礼, 野々村 照雄, 桜谷保之, 角谷 晃司, 大津康成, 土佐幸雄, 眞山滋志, 平成15 年度日本植物病理学会関西支部(奈良),   2003 10 , 平成15 年度日本植物病理学会関西支部(奈良)
    Summary:本実験では、トマト葉圏から幼虫に対して食害抑制効果を示す細菌の分離を試み、生物防除資材への適用を検討した。トマト葉圏から分離された細菌を幼虫の経口から直接導入し、一定期間トマト葉を摂食させることにより食害抑制効果を示す細菌の選抜を行った。
  • Biological control of powdery mildew and gray mold of tomato by chitinolytic phylloepiphytes ? Stable phylloplane colonization by entomopathegenic bacterium Pseudomonas fluorescens KPM.018P, 豊田 秀吉, 東希望, 松田 克礼, 藤原圭一, 野々村 照雄, 澤邊 昭義, 角谷 晃司, 大津康成, 土佐幸雄, 眞山滋志, 平成15 年度日本植物病理学会関西支部(奈良),   2003 10 , 平成15 年度日本植物病理学会関西支部(奈良)
    Summary:本実験では、KPM.18P の特性を調査し、さらに定着能力を強化することとした。一般に高度葉面定着を示す細菌は生物界面活性剤を生産することが報告されていることから、本細菌の生物界面活性剤生産能について検討した。
  • Produce of physiologically functional plants introduced the CD98 gene for tumor-specific protein, 角谷 晃司, 豊田 秀吉, 森浦展行, 岩崎健太, 畑太一, 益子 高, 日本植物生理学会2003年度年会(大阪),   2003 03 , 日本植物生理学会2003年度年会(大阪)
    Summary:ガン細胞特異タンパク質であるCD98の遺伝子をミヤコグサに導入し、その遺伝子の発現とタンパク質の翻訳について報告した。これらの植物はガン予防を目的とした経口ワクチンとしての利用が示唆された。
  • Microinjection.based in situ PCR for gene cloning in targeted single cells of barley coleoptile(?)gene-expression detection mediated by RT.PCR of needle-sucked cell contents, 豊田 秀吉, 松田 克礼, 鮫島武, 和夛泉季, 野々村 照雄, 藤田和久, 角谷 晃司, 平成15年度日本植物病理学会大会(東京),   2003 03 , 平成15年度日本植物病理学会大会(東京)
    Summary:オオムギうどんこ病菌の感染を受けた子葉鞘細胞およびその隣接細胞に単一細胞RT.PCR法を適用し、感染特異的に発現する遺伝子の解析を進めている。本研究では、単一細胞から細胞内容物を吸引し、そこに存在するmRNAを鋳型としたRT.PCRを試みた。
  • Suppression of Fusarium oxysporum by 5.fluoroindole(5.FI)and molecular breeding of 5.FI resistant plants(?)Cloning of modified anthranilate synthetase gene(masa)from Arabido psis thaliane, 豊田 秀吉, 野々村 照雄, 福元健志, 松田 克礼, 小牧伸一郎, 角谷 晃司, 平成15年度日本植物病理学会大会(東京),   2003 03 , 平成15年度日本植物病理学会大会(東京)
    Summary:5.フルオロインドール(5.FI)耐性植物の作出を目的として、シロイヌナズナ(品種コロンビア)からアントラニル酸合成酵素遺伝子をクローニングした後、フィードバック阻害を受けない変異遺伝子の作製を試みた。
  • Pricking RT?PCR for cloning genes expressed in targeted infection structures of Blumeria graminis f. sp. hordei?, 豊田 秀吉, 鮫島武, 松田 克礼, 藤田和久, 木多景子, 野々村 照雄, 角谷 晃司, 池田成志, 平成14年度日本植物病理学会関西部会(三重),   2002 09 , 平成14年度日本植物病理学会関西部会(三重)
    Summary:本菌は絶対寄生菌であることから、特定の分子ステージにあるうどんこ病菌を大量に得ることは非常に困難であり、それぞれの器官で発現する遺伝子の解析が必ずしも容易ではない。本実験ではPricking RT?PCR法を用い、単一器官を標的として発現遺伝子の解析を行った。
  • Microinjection based in situ PCR for gene cloning in targeted single cells of barley coleoptile (?) Control of gene amplification by different primers and annealing conditions, 豊田 秀吉, 平井康晴, 松田 克礼, 相場政人, 藤田和久, 野々村 照雄, 角谷 晃司, 平成 13 年度日本植物病理学会関西部会 (高知),   2001 10 , 平成 13 年度日本植物病理学会関西部会 (高知)
    Summary:オオムギ子葉鞘の単一細胞から RT PCR により遺伝子をクローニングするため、 基礎的条件として常時発現している遺伝子のプライマーを構築し、 PCR 時の反応温度および増幅量について検討した。
  • Purification and property of UDP glucuronosyltransferase in the root of the licorice (Glycyrrhiza glabra L.), 角谷 晃司, 第 19 回日本植物細胞分子生物学会 (東京),   2001 07 , 第 19 回日本植物細胞分子生物学会 (東京)
    Summary:カンゾウの薬用成分であるグリチルリチンの生合成を明らかにするため、 それらに関与するグルクロン酸転移酵素を生根より抽出、 精製した。 約 50 KPa の分子量のタンパク質であり、 作用 PH が異なる 2 種のタンパクがグリチルリチン生合成に関与していた。
  • Purification and characterization of UDP glucuronosyl transferase of licorice, 角谷 晃司, 友田勝巳, 渡辺斉, 日本農芸化学会 2001 年度大会 (京都),   2001 03 , 日本農芸化学会 2001 年度大会 (京都)
    Summary:甘草の主成分であるグリチルリチンは、 グリチルレチン酸の 3 位にグルクロン酸 2 分子が結合したトリテルペンサボニンである。 今回、 グリチルリチン生合成に関与するグルクロン酸転移酵素を生根より精製し、 その諸性質について詳細な検討を試みた。

Misc

  • メロンうどんこ病菌単一菌叢あたりの生涯分生子生産数の測定, 野々村照雄, 中村亮介, 瀧川義浩, 角谷晃司, 松田克礼, 日本菌学会大会講演要旨集, 62nd, 72,   2018 05 01 , http://jglobal.jst.go.jp/public/201802263977286178
  • ショウジョウバエを用いたクロシン摂食によるブルーライト(BL)傷害抑制効果, 天野耕政, 角谷晃司, 角谷晃司, 日本生薬学会年会講演要旨集, 64th, 261, 261,   2017 08 25 , http://jglobal.jst.go.jp/public/201802265717855631
  • メロンうどんこ病菌の分生子柄形成に及ぼす光の影響, 野々村照雄, 西村祥吾, 瀧川義浩, 角谷晃司, 松田克礼, 日本菌学会大会講演要旨集, 60th, 76,   2016 09 03 , http://jglobal.jst.go.jp/public/201602296552331346
  • トガリスズメバチタケ(Ophiocordyceps oxycephala)が生産するトリプシン様プロテアーゼの諸性質について, 吉田真梨奈, 角谷晃司, 日本生薬学会年会講演要旨集, 63rd, 131, 131,   2016 08 25 , http://jglobal.jst.go.jp/public/201602225537671191
  • 蟾酥成分がショウジョウバエの寿命に与える影響, 中村恭子, 津川舜, 清水康晴, 角谷晃司, 角谷晃司, 日本生薬学会年会講演要旨集, 63rd, 191, 191,   2016 08 25 , http://jglobal.jst.go.jp/public/201602282925905016
  • Crocetin生産を目的としたZCD遺伝子の活用, 香川真由美, 古川明典, 市川智康, 阿野友里, 岡部真依, 森井由梨, 角谷晃司, 日本生薬学会年会講演要旨集, 63rd, 108, 108,   2016 08 25 , http://jglobal.jst.go.jp/public/201602286097727784
  • ゼアキサンチン高含量素材を用いた組換えZCDによるcrocetin産生, 市川智康, 古川明典, 阿野友里, 香川真由美, 森井由梨, 角谷晃司, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 34th, 171,   2016 08 20 , http://jglobal.jst.go.jp/public/201602212371920696
  • サフラン柱頭様組織の分化とクロシン産生, 角谷晃司, 森健太郎, 石川理英, 安尾みなみ, 古川明典, 中村恭子, 福田浩三, 日本生薬学会年会講演要旨集, 62nd, 215, 215,   2015 08 31 , http://jglobal.jst.go.jp/public/201602242030918338
  • Analysis of whitefly longevity aiming at the establishment of simple experimental systems for studying anti-aging mechanisms, 瀧川義浩, 坂本純一, 松田克礼, 野々村照雄, 豊田秀吉, 角谷晃司, 近畿大学先端技術総合研究所紀要, 20, 43, 48,   2015 03 31 , http://jglobal.jst.go.jp/public/201502235177925698
  • Analysis of whitefly longevity aiming at the establishment of simple experimental systems for studying anti-aging mechanisms, 瀧川 義浩, 坂本 純一, 松田 克礼, 野々村 照雄, 豊田 秀吉, 角谷 晃司, Memoirs of Institute of Advanced Technology, Kinki University, 20, 43, 48,   2015 03 , http://ci.nii.ac.jp/naid/120005736507
    Summary:[要旨] 本研究は, アンチエイジング解析を目的として, タバココナジラミを使用した寿命評価システムの確立について検討した. すなわち, 異なる寄主植物であるインゲンとキャベツで飼育したタバココナジラミの寿命について, 成虫の生存率が100% を下回る時期(ポイントA ), 50% ならびに0% に到達した時期(ポイントB およびC)の飼育日数を調査したところ, 幼虫期の環境より, むしろ成虫期における飼育環境がタバココナジラミの生存に影響を及ぼす傾向が認められ, 本システムの利用の有効性が確認された. [Abstract] In the present study, we described that whitefly was suitable to study anti-aging mechanisms. Moreover, we examined point A, B and C represent the days showing the survival rates of 100, 50 and 0%. In comparative analysis of survivability of the whitefly on different combinations of host plants before and after emergence and stage I and II, there was difference in the test points between pea bean and cabbage seedlings in adult stage.近畿大学先端技術総合研究所紀要編集委員会
  • ResveratrolおよびFisetinにより誘導する抗加齢関連遺伝子の発現解析, 角谷晃司, 西川恵未, 中村恵実, 中村恭子, 瀧川義浩, 早川堯夫, 日本生薬学会年会講演要旨集, 61st, 281,   2014 08 27 , http://jglobal.jst.go.jp/public/201402235757842385
  • ResveratrolおよびFisetinによる誘導する抗加齢関連遺伝子の発現解析, 角谷 晃司, 西川 恵未, 中村 恵実, 中村 恭子, 瀧川 義浩, 早川 堯夫, 日本生薬学会年会講演要旨集, 61回, 281, 281,   2014 08
  • サフラン茎組織から分化させた柱頭様組織のクロシン産生, 森健太郎, 松尾早希子, 安村美紀, 古川明典, 中村恭子, 福田浩三, 角谷晃司, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 32nd, 103,   2014 07 30 , http://jglobal.jst.go.jp/public/201402255632695837
  • カンゾウのフラボノイド系グルコース転移酵素遺伝子の単離と発現解析, 古川明典, 宮崎潤一郎, 桑原侑己, 角谷晃司, 日本生薬学会年会講演要旨集, 60th, 219, 219,   2013 08 20 , http://jglobal.jst.go.jp/public/201402206867943519
  • グルコアルカロイドtomatineのNa+/K+ATPase阻害効果及び寿命延長効果の検討, 森健太郎, 田中優華, 角谷晃司, 日本生薬学会年会講演要旨集, 60th, 160,   2013 08 20 , http://jglobal.jst.go.jp/public/201402292784525360
  • グルコアルカロイドtomatineのNa+/K+ATPase阻害効果及び寿命延長効果の検討, 森 健太郎, 田中 優華, 角谷 晃司, 日本生薬学会年会講演要旨集, 60回, 160, 160,   2013 08
  • Identification of Bemisia tabaci biotypes and detection of TYLCV from diseased tomato plants and viruliferous biotype Q whiteflies, 松田克礼, 瀬戸本真実, 吉本尚民, 野々村照雄, 角谷晃司, 瀧川義浩, 豊田秀吉, 近畿大学農学部紀要, 46, 55, 58,   2013 03 30 , http://jglobal.jst.go.jp/public/201302290003290770
  • カンゾウ(Glycyrrhiza glabra L.)のフラボノイド系ポリフェノール配糖化酵素遺伝子の単離と解析, 桑原侑己, 東江直樹, 瀧川義浩, 早川堯夫, 角谷晃司, 日本農芸化学会大会講演要旨集(Web), 2013, 2C16P15 (WEB ONLY),   2013 03 05 , http://jglobal.jst.go.jp/public/201302242332078842
  • 〈Original〉Identification of Bemisia tabaci biotypes and detection of TYLCV from diseased tomato plants and viruliferous biotype Q whiteflies, 松田 克礼, 瀬戸本 真実, 吉本 尚民, 野々村 照雄, 角谷 晃司, 瀧川 義浩, 豊田 秀吉, 近畿大学農学部紀要 = Memoirs of the Faculty of Agriculture of Kinki University, 46, 55, 58,   2013 03 01 , http://ci.nii.ac.jp/naid/120005734780
    Summary:[Synopsis] A typical yellow leaf curl disease severely occurred in tomato plants of our greenhouses. The symptom was similar to the disease caused by tomato yellow leaf curl virus (TYLCV). To identify a causal pathogen, tomato plants showing the typical symptom and whiteflies on the plants were tested for their viruliferousness with a commercial TYLCV detection kit. All tomato plants and whiteflies tested were positive for infection with TYLCV. Moreover, biotypes of collected whiteflies were examined using the same PCR method. The present survey revealed that 31 whiteflies were biotype B and five whiteflies were biotype Q of Bemisia tabaci.
  • 冬虫夏草(Cordyceps sinensis)菌糸抽出タンパク質のMatrix metalloproteinase阻害作用, 角谷晃司, 鎌田夏樹, 瀧川義浩, 日本生薬学会年会講演要旨集, 59th, 140, 140,   2012 08 31 , http://jglobal.jst.go.jp/public/201302205067201375
  • 各種サラシア属植物のITS領域の比較, 角谷晃司, 塩崎有里, 石伏史明, 谷恭輔, 瀧川義浩, 村岡修, 吉川雅之, 日本薬学会年会要旨集, 132nd, 2, 202,   2012 03 05 , http://jglobal.jst.go.jp/public/201202277641414335
  • ナス科植物アルカロイドトマチンの細胞増殖抑制効果と寿命延長効果, 角谷晃司, 立川由佳, 池内隼人, 小西実可子, 田中優華, 瀧川義浩, 日本生薬学会年会講演要旨集, 58th, 218, 218,   2011 09 01 , http://jglobal.jst.go.jp/public/201302268134603230
  • Cultivation of Wild Tomato Species and Preliminary Extraction of Nutraceutical Substances Useful for Prolongment of Insect Longevity, 瀧川義浩, 河村洋樹, 角谷晃司, 野々村照雄, 松田克礼, 豊田秀吉, 近畿大学先端技術総合研究所紀要, 16, 7, 18,   2011 03 31 , http://jglobal.jst.go.jp/public/201102212707811681
  • ナス科植物アルカロイドの腫瘍細胞増殖抑制効果とアンチエイジング効果の検討, 角谷晃司, 辻村亜貴, 田中宏範, 木坊子敏生, 田中優華, 瀧川義浩, 日本農芸化学会大会講演要旨集, 2011, 222,   2011 03 05 , http://jglobal.jst.go.jp/public/201102205661267926
  • トマチンの腫瘍細胞増殖抑制効果とアンチエイジング効果, 角谷晃司, 辻村亜貴, 田中宏範, 木坊子敏生, 田中優華, 瀧川義浩, 日本薬学会年会要旨集, 131st, 2, 259, 259,   2011 03 05 , http://jglobal.jst.go.jp/public/201102207487790102
  • 各種サラシア属植物のITS領域の遺伝子解析, 角谷晃司, 石伏史明, 谷恭輔, 瀧川義浩, 村岡修, 吉岡雅之, 日本農芸化学会大会講演要旨集, 2011, 304,   2011 03 05 , http://jglobal.jst.go.jp/public/201102209787230896
  • 静電場スクリーンを用いた花粉症予防に関する研究, 桑原侑己, 今関尭之, 角谷晃司, 瀧川義浩, 野々村照雄, 松田克礼, 豊田秀吉, 日本農芸化学会大会講演要旨集, 2011, 167,   2011 03 05 , http://jglobal.jst.go.jp/public/201102278181825675
  • トマチンの腫瘍細胞増殖抑制効果とアンチエイジング効果の検討, 角谷晃司, 辻村亜貴, 田中宏範, 木坊子敏生, 瀧川義浩, 日本農芸化学会関西支部講演会講演要旨集, 466th, 49,   2010 10 02 , http://jglobal.jst.go.jp/public/201002281920350803
  • トマトうどんこ病菌の分生子柄に形成された偽鎖生分生子は高い発芽力を有し同調的に分化する, 野々村照雄, 松田克礼, 角谷晃司, 瀧川義浩, 豊田秀吉, 日本植物病理学会大会プログラム・講演要旨予稿集, 2010, 70,   2010 03 30 , http://jglobal.jst.go.jp/public/201002237000053000
  • 大和シャクヤクの効率的な育苗方法, 角谷晃司, 大野加奈, 日本薬学会年会要旨集, 130th, 2, 227,   2010 03 05 , http://jglobal.jst.go.jp/public/201002236066967855
  • 既知ポリフェノール類のSIRT1活性促進効果, 角谷晃司, 中村文亮, 吉田明寿香, 瀧川義浩, 早川堯夫, 日本薬学会年会要旨集, 129th, 3, 160, 160,   2009 03 05 , http://jglobal.jst.go.jp/public/200902203337813968
  • 各種エリシター処理によるResveratrol生合成関連遺伝子の発現解析, 角谷晃司, 岩崎友美, 瀧川義浩, 早川堯夫, 日本農芸化学会大会講演要旨集, 2009, 303,   2009 03 05 , http://jglobal.jst.go.jp/public/200902231737339681
  • 大和シャクヤクの生長点培養, 角谷晃司, 豊坂加奈, 福田眞三, 日本生薬学会年会講演要旨集, 55th, 144,   2008 09 01 , http://jglobal.jst.go.jp/public/200902243393192447
  • アルゴンエキシマランプ照射によるヒメツリガネゴケの生育に及ぼす影響, 瀧川義浩, 戸出真由美, 瀧川靖雄, 佐々木亘, 角谷晃司, 日本農芸化学会大会講演要旨集, 2008, 59,   2008 03 05 , http://jglobal.jst.go.jp/public/200902250332608396
  • 冬虫夏草(Cordyceps sinensis)菌糸の分離と培養条件の検討, 角谷晃司, 齋藤裕介, 瀧川義浩, 森川敏生, 二宮清文, 村岡修, 吉川雅之, 掛樋一晃, 日本農芸化学会大会講演要旨集, 2008, 83,   2008 03 05 , http://jglobal.jst.go.jp/public/200902278310222500
  • 大和シャクヤクの生長点培養における褐変化の抑制, 角谷晃司, 豊坂加奈, 福田眞三, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 26th, 205,   2008 , http://jglobal.jst.go.jp/public/201002243711323719
  • Resveratrol合成関連酵素を利用したResveratrolおよびその前駆体4-coumaroyl CoAの生合成, 角谷 晃司, 野々村 照雄, 松田 克礼, 豊田 秀吉, 瀧川 義浩, 日本生薬学会年会講演要旨集, 54回, 98, 98,   2007 09
  • Resveratrol合成関連酵素を利用したResvertarolおよびその前駆体4‐coumaroyl CoAの生合成, 角谷晃司, 永本真也, 野々村照雄, 松田克礼, 豊田秀吉, 瀧川義浩, 日本生薬学会年会講演要旨集, 54th, 98,   2007 09 01 , http://jglobal.jst.go.jp/public/200902216138345798
  • 腫瘍細胞表面で発現するCD98hc抗原領域の探索, 角谷晃司, 瀧川義浩, 前田博祥, 益子高, 豊田秀吉, 松田克礼, 野々村照雄, 日本農芸化学会大会講演要旨集, 2007, 179,   2007 03 05 , http://jglobal.jst.go.jp/public/200902215833962750
  • 抗体医薬品のコストダウンを目指した基礎検討, 段林正明, 池田達哉, 角谷晃司, 伊藤邦彦, 橋本嘉幸, 益子和恵, 益子高, 日本薬学会年会要旨集, 127th, 2, 31, 31,   2007 03 05 , http://jglobal.jst.go.jp/public/200902219928412370
  • 酵母細胞膜表面で固定化したResveratrol合成関連酵素による,Resveratrolおよびその前駆体4‐coumaroyl CoAの生合成, 角谷晃司, 永本真也, 谷本惠津子, 野々村照雄, 松田克礼, 豊田秀吉, 日本農芸化学会大会講演要旨集, 2007, 38,   2007 03 05 , http://jglobal.jst.go.jp/public/200902228483062560
  • RAPD分析による大和トウキと北海トウキの識別, 角谷晃司, 青山知佳, 野々村照雄, 松田克礼, 豊田秀吉, 橋本隆, 大木宏之, 福田眞三, 福田浩三, 日本生薬学会年会講演要旨集, 53rd, 138, 138,   2006 09 01 , http://jglobal.jst.go.jp/public/200902228442814344
  • アルギニントランスポーターを利用した出芽酵母細胞膜上における固定化酵素の作出, 永本真也, 角谷晃司, 野々村照雄, 松田克礼, 豊田秀吉, 日本薬学会年会要旨集, 126th, 3, 18, 18,   2006 03 06 , http://jglobal.jst.go.jp/public/200902204277566547
  • Development of DNA Markers for Discriminating Isolates of Fusarium oxysporum f. sp. radicislycopersici on the Basis of Single Nucleotide Polymorphism of Polygalacuturonase Genes (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Paper, Okada M, Nonomura T, Kakutani K, Matsuda Y, Toyoda H, Annals of the Phytopathological Society of Japan, 72, 1,   2006 02 25 , http://ci.nii.ac.jp/naid/110004702713
  • Selection and Production of Functional Plant using Molecular Techniques,   2005 11
  • (147) Development of DNA Markers for Discriminating Isolates of Fusarium oxysporum f. sp. radicislycopersici on the Basis of Nucleotide Substitution of Endo-and Exo-polygalacturonase Genes(Abstracts of the Papers Presented at the 2005 Annual Meeting in Sh, Okada M, Nonomura T, Tajima H, Sanada M, Kakutani K, Matsuda Y, Toyoda H, Annals of the Phytopathological Society of Japan, 71, 3,   2005 08 25 , http://ci.nii.ac.jp/naid/110002770113
  • (174) Consecutive Observation of Germination by Tomato Powdery Mildew Oidium neolycopersici with a High-Fidelity Digital Microscope(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka), Oichi W, Matsuda Y, Sameshima T, Nonomura T, Kakutani K, Nishimura H, Kusakari S, Toyoda H, Annals of the Phytopathological Society of Japan, 71, 3,   2005 08 25 , http://ci.nii.ac.jp/naid/110002770140
  • (175) Screening of Wild Lycopersicon Species for Resistance to Japanese Isolate of Tomato Powdery Mildew Oidium neolycopersici(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka), Sakano Y, Matsuda Y, Mori Y, Tarumoto K, Nonomura T, Kakutani K, Nishimura H, Kusakari S, Toyoda H, Annals of the Phytopathological Society of Japan, 71, 3, 231, 232,   2005 08 25 , 10.1270/jsbbs.55.355, http://ci.nii.ac.jp/naid/110002770141
  • (176) Suppression of Chitinase Gene Expression Induced in Detached Inner Epidermis by Invading Conidia of Barley Powdery Mildew(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka), Matsuda Y, Fujita K, Moriura N, Wada M, Nonomura T, Kakutani K, Toyoda H, Annals of the Phytopathological Society of Japan, 71, 3,   2005 08 25 , http://ci.nii.ac.jp/naid/110002770142
  • Selection and Production of Functional Plant using Molecular Techniques,   2005 06
  • Direct RT-PCR amplification of mature mRNAs in cytoplasm micropipetted from barley coleoptile epidermal cell-A model system for analyzing gene expression in host cells attacked by powdery mildew, Moriura Nobuyuki, Matsuda Yoshinori, Fujita Kazuhisa, Wada Mizuki, Kakutani Koji, Toyoda Hideyoshi, Memoirs of the Faculty of Agriculture of Kinki University, 38, 9, 18,   2005 03 31 , http://ci.nii.ac.jp/naid/110004615569
    Summary:Single-cell RT-PCR was used to detect gene expression in situ in single selected cells of detached barley coleoptile epidermis inoculated with Blumeria graminis f. sp. hordei. The cytoplasm was removed with a micropipette using a light microscope and directly used for RT-PCR, followed by nested PCR. Three intron-containing genes, vacuolar ATPase B-subunit gene, cytosolic triosephosphate isomerase gene, and glycolitic glyceraldehyde-3-phosphate dehydrogenase gene were constantly expressed in this tissue and were therefore used as indicators, because shorter-size PCR-products produced by splicing are easy to detect. By simultaneously amplifying both target and indicator genes, the transcription of some stimuli-induced genes such as chitinase 2 and acidic β-1, 3-glucanase genes could be precisely detected in powdery-mildew-invaded and noninvaded, neighboring cells of the coleoptile epidermis. In addition, micropipetting only the cytoplasm without the nucleus prevented contamination with genomic DNA, which leads to miss-amplification of corresponding genomic DNA sequences of the intron-less genes during RT-PCR and subsequent nested PCR. Thus, this technique can be successfully used with coleoptile epidermal cells on which the powdery mildew fungus has attempted to invade and/or form a haustorium or with epidermal cells in the vicinity of infected cells.
  • トマトおけるTy3/gypsy型レトロトランスポゾン逆転写酵素領域の分子進化系統解析, 角谷晃司, 笹尾真理, 南条綾子, 中川真樹, 高畑智香子, 樫尾智子, 玉井智子, 森浦展行, 田中正起, 近畿大学薬学総合研究所紀要, 13, 103, 111,   2005 03 05 , http://jglobal.jst.go.jp/public/200902266152128390
  • Molecular Evolution Analysis of the Reverse Transcriptase Region of the Ty3/gypsy Retrotransposons in Lycopersicon esculentum, Kakutani Koji, Sasao Mari, Nanjo Ayako, Bulletin of Pharmaceutical Research and Technology Institute, 13, 103, 111,   2005 03 05 , http://ci.nii.ac.jp/naid/40007000279
  • (66) Suppression of Fusarium oxysporum by 5-fluoroindole (5-FI) and Molecular Breeding of 5-FI Resistant Plants (V). : Analysis of Expression of a Modified Anthranilate Synthetase Gene Introduced into Arabidopsis and Tomato Plants(Abstracts of the Papers, Komaki S, Nonomura T, Kakutani K, Matsuda Y, Toyoda H, Annals of the Phytopathological Society of Japan, 70, 3,   2004 08 25 , http://ci.nii.ac.jp/naid/110002767726
  • (119) Phylogenetic Analysis of Fusarium oxysporum with Four Polygalacuturonase Genes(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004), Miyamoto Y, Nonomura T, Okada M, Kakutani K, Matsuda Y, Toyoda H, Annals of the Phytopathological Society of Japan, 70, 3,   2004 08 25 , http://ci.nii.ac.jp/naid/110002767779
  • (158) Consecutive Monitoring for Condiogenesis by Oidium neolycopersici Tomato Leaves with a High-Fidelity Digital Microscope(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004), Oichi W, Matsuda Y, Sameshima T, Nonomura T, Kakutani K, Nishimura H, Kusakari S, Toyoda H, Annals of the Phytopathological Society of Japan, 70, 3, 232, 233,   2004 08 25 , 10.1007/s10327-004-0136-5, http://ci.nii.ac.jp/naid/110002767818
  • (159) Molecular Discrimination of Different Powdery Mildews Simultaneously Attacking Plant Leaves by Nested-PCR Amplification of rDNA ITS Sequences from Single Appressorium-forming Conidia(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004), Inoue K., Matsuda Y., Sameshima T., Nonomura T., Kakutani K., Nishimura H., Kusakari S., Toyoda H., Annals of the Phytopathological Society of Japan, 70, 3, 233, 233,   2004 08 25 , http://ci.nii.ac.jp/naid/110002767819
  • (160) Digital Microscopic Observation of Naked Powdery Mildews on Leaves and Direct PCR-amplification of Mature mRNAs from Infecting Single Conidia(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004), Sameshima T, Matsuda Y, Fujita K, Oichi W, Nonomura T, Kakutani K, Nishimura H, Kusakari S, Toyoda H, Annals of the Phytopathological Society of Japan, 70, 3,   2004 08 25 , http://ci.nii.ac.jp/naid/110002767820
  • ミヤコグサ(Lotus japonicus)におけるトランスポーター遺伝子の発現解析, 瀧川義浩, 角谷晃司, 森浦展行, 横山大聡, 野々村照雄, 松田克礼, 豊田秀吉, 日本植物生理学会年会要旨集, 45th, 243,   2004 03 20 , 10.14841/jspp.2004.0.560.0, http://jglobal.jst.go.jp/public/200902229836947370
  • ミヤコグサにおけるトランスポーター遺伝子の発現解析, 森浦展行, 角谷晃司, 瀧川義浩, 横山大聡, 佐藤修正, 浅水恵里香, 田畑哲之, 益子高, 野々村照雄, 日本農芸化学会大会講演要旨集, 2004, 303,   2004 03 05 , http://jglobal.jst.go.jp/public/200902262461919240
  • 異なる支持体を用いたカンゾウの水耕栽培によるグリチルリチン生産, 角谷晃司, 近畿大学薬学総合研究所紀要, 12, 133, 138,   2004 03 05 , http://jglobal.jst.go.jp/public/200902266301194112
  • 緑色蛍光タンパク質生産遺伝子発現を利用したトマト毛状根形成効率の遺伝子工学的解析, 福元健志, 野々村照雄, 松田克礼, 小牧伸一郎, 森浦展行, 沢辺昭義, 角谷晃司, 豊田秀吉, 近畿大学薬学総合研究所紀要, 12, 99, 106,   2004 03 05 , http://jglobal.jst.go.jp/public/200902266840074538
  • Glycyrrhizin Production of Licorice by Nutricultures using Several Material, Kakutani Koji, Bulletin of Pharmaceutical Research and Technology Institute, 12, 133, 138,   2004 03 05 , http://ci.nii.ac.jp/naid/110001180584
  • (47) Analysis of Variations in Reverse Transcriptase Domain of Gypsy-like Retrotransposon in Tomato (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Jap, Tanaka M, Kakutani K, Toda A, Nonomura T, Matsuda Y, Toyoda H, Annals of the Phytopathological Society of Japan, 70, 1, 68, 69,   2004 02 25 , http://ci.nii.ac.jp/naid/110002767574
  • (50) Biological Control of Powdery Mildew and Gray Mold of Tomato by Chitinolytic Phylloepiphytes (V)Biological Control of Phyllophagous Ladybird Beetles by Chitinolytic Bacteria (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Pa, Matsuda Y, Otsu Y, Fujiwara K, Nonomura T, Sakuratani Y, Kakutani K, Tosa Y, Mayama S, Toyoda H, Annals of the Phytopathological Society of Japan, 70, 1,   2004 02 25 , http://ci.nii.ac.jp/naid/110002767577
  • (51) Biological Control of Powdery Mildew and Gray Mold of Tomato by Chitinolytic Phylloepiphytes (VI) Biological Control of Phyllophagous Ladybird Beetles by Entomopathogenic Bacteria (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts, Fujiwara K, Otsu Y, Matsuda Y, Nonomura T, Sakuratani Y, Kakutani K, Tosa Y, Mayama S, Toyoda H, Annals of the Phytopathological Society of Japan, 70, 1, 69, 70,   2004 02 25 , http://ci.nii.ac.jp/naid/110002767578
  • (52) Biological Control of Powdery Mildew and Gray Mold of Tomato by Chitinolytic Phylloepiphytes (VII) : Stable Phylloplane Colonization by Entomopathogenic Bacterium Pseudomonas fluorescens KPM-018P (Abstracts Presented at the Meeting of the Kansai Divi, Azuma N, Matsuda Y, Fujiwara K, Otsu Y, Nonomura T, Kakutani K, Sawabe A, Tosa Y, Mayama S, Toyoda H, Annals of the Phytopathological Society of Japan, 70, 1,   2004 02 25 , http://ci.nii.ac.jp/naid/110002767579
  • Expression analysis of transporter genes in Lotus japonicus, Takikawa Yoshihiro, Kakutani Koji, Moriura Nobuyuki, Yokoyama Hiroaki, Nonomura Teruo, Matsuda Yoshinori, Toyoda Hideyoshi, Plant and Cell Physiology Supplement, 2004, 0, 560, 560,   2004 , 10.14841/jspp.2004.0.560.0, http://ci.nii.ac.jp/naid/130006988595
    Summary:We attempted to analyze gene expression in the absence and presence of nitrogen using a <I>Lotus japonicus</I> Gifu B-129. For the gene expression analysis by RT-PCR, we used 12 transporter genes of <I>Lotus japonicus</I> provided by Kazusa DNA Research Institute. Namely, plants were grown on nitrogen-free medium for 60 days and total RNA was extracted from the roots, stems and leaves for RT-PCR, respectively. As a result, of the 12 genes, 6 genes exhibited constitutive expression in roots, stems and leaves, respectively. Moreover, of remaining 6 genes, each of 2 genes was specifically expressed in leaves and roots. In contrast, despite nitrogen-supplement, 6 genes exhibited constitutive expression in roots, stems and leaves, respectively. However, of remaining 6 genes, the expression level of 4 genes was suppressed within 24 hrs in roots and leaves in the presence of nitrogen. These genes are highly identical to amino acid and nitrate transporters.
  • PCR法で調べる遺伝子の情報, 角谷晃司, 日本農業気象学会全国大会日本生物環境調節学会大会合同大会講演要旨, 2003, 414,   2003 09 08 , http://jglobal.jst.go.jp/public/200902263784870734
  • Microinjection-based in situ PCR for Gene Cloning in Targeted Single Cells of Barley Coleoptile (IX) Gene-Expression Detection Mediated by RT-PCR of Needlesucked Cell Contents(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo), Fujita K, Matsuda Y, Samesima T, Wada M, Nonomura T, Kakutani K, Toyoda H, Annals of the Phytopathological Society of Japan, 69, 3,   2003 08 25 , http://ci.nii.ac.jp/naid/110002767062
  • Morphological and Molecular Characterization for a Japanese Isolate of Tomato Powdery Mildew Oidium neolycopersici and Its Host Range(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo), Kashimoto K, Matsuda Y, Matsutani K, Samejima T, Kakutani K, Nonomura T, Okada K, Kusakari S, Nakata K, Takamatsu S, Toyoda H, Annals of the Phytopathological Society of Japan, 69, 3,   2003 08 25 , http://ci.nii.ac.jp/naid/110002767073
  • Suppression of Fusarium oxysporum by 5-fluoroindole (5-FI) and Molecoular Breeding of 5-FI Resistant Plants (IX). Cloning of Modified Anthranilate Synthetase Gene (masa) from Arabidopsis thaliana(Abstracts of the Papers Presented at the 2003 Annual Meetin, Komaki S, Nonomura T, Fukumoto T, Kakutani K, Matsuda Y, Toyoda H, Annals of the Phytopathological Society of Japan, 69, 3,   2003 08 25 , http://ci.nii.ac.jp/naid/110002767200
  • 甘草(Glycyrrhiza glabra L.)のグルクロン酸転移酵素遺伝子の解析, 角谷晃司, 近畿大学薬学総合研究所紀要, 11, 105, 110,   2003 03 05 , http://jglobal.jst.go.jp/public/200902249345386955
  • Pricking RT-PCR for Cloning Genes Expressed in Targeted Infection Structures of Blumeria graminis f. sp. hordei (I), Sameshima T, Matuda Y, Fujita K, Kida K, Ikeda S, Kakutani K, Nonomura T, Toyoda H, Annals of the Phytopathological Society of Japan, 69, 1, 57, 58,   2003 02 25 , http://ci.nii.ac.jp/naid/110002716742
    Summary:本菌は絶対寄生菌であることから,特定の分子ステージにあるうどんこ病菌を大量に得ることは非常に困難であり,その為,それぞれの器官で発現する遺伝子の解析が必ずしも容易ではない.そこで,本実験では,Pricking RT-PCR法を用い,単一の器官を標的として,それらで発現する遺伝子の解析を試みた.まず,あらかじめ作製したEST(うどんこ病菌感染イタリアンライグラスから作製したライブラリー)をクラスター分類し,塩基配列を決定した11,137個のグローンから,構成的に発現するキチン合成酵素遺伝子(chs1,chs2)とβ-tubulin遺伝子(tub2)のホモログを得た.次に,これらを特異的に増幅するPCR-プライマーを分生胞子や付着器に導入し,得られたPCR産物の塩基配列を決定したところ,遺伝子内のイントロン配列がスプライスされたPCR産物が得られた.このような結果から,本法はin situ・RT-PCR法として,うどんこ病菌の単一の分化器官における遺伝子発現解析に適用できるものと結論した.
  • Produce of physiologically functional plants introduced the CD98 gene for tumor-specific protein, Kakutani Koji, Moriura Nobuyuki, Iwasaki Kenta, Hata Taichi, Masuko Takasi, Toyoda Hideyoshi, Plant and Cell Physiology Supplement, 2003, 0, 252, 252,   2003 , 10.14841/jspp.2003.0.252.0, http://ci.nii.ac.jp/naid/130006989242
    Summary:In an attempt to produce physiologically functional plants exhibiting an anti-cancer effect, we prepared transgenic plants transformed with the CD98 gene for a tumor-specific, membrane-located protein. The CD98 protein plays a significant role on amino acid transport and integrin-mediated adhesion, and is strongly produced in tumorous cells of kidney, small intestine, testis and ovary. In this study, the CD98 gene was introduced into <I>Louts japonicus</I> using an <I>Agrobacterium</I> -mediated method, and its expression was detected by RT-PCR and Western blotting analysis. As a result, hygromycin-resistance lines selected were shown to successfully transcribe the introduced gene and translate the CD98 protein. Thus, the present study suggests that our approach provides an experimental basis for production of edible vaccine against CD98.
  • Cloning of Ty1/copia-like and Ty3/gyspy-like retrotransposons in tomato plants, KAKUTANI K, NANJO A, SASAO M, MATSUDA Y, NONOMURA T, TOYODA H, 育種学研究 = Breeding research, 4,   2002 08 26 , http://ci.nii.ac.jp/naid/10010403276
  • (26)Microinjection-based in situ PCR for Gene Cloning in Targeted Single Cells of Barley Coleoptile (VIII) Utilization of Constitutively Expressed Genes as a Marker for an Effective in situ RT-PCR, Hirai Y, Matsuda Y, Aiba K, Fujita K, Sameshima T, Kakutani K, Nonomura T, Toyoda H, Annals of the Phytopathological Society of Japan, 68, 2,   2002 08 25 , http://ci.nii.ac.jp/naid/110002752616
  • (96)In vitro Suppression of Mycelial Growth of Fusarium oxysporum by Extracellular Chitosanase of Sphingobacterium multivorum and Cloning of the Chitosanase Gene, Iida Y, Matsuda Y, Shinogi T, Kakutani K, Nonomura T, Toyoda H, Annals of the Phytopathological Society of Japan, 68, 2,   2002 08 25 , http://ci.nii.ac.jp/naid/110002752686
  • (272)Cloning of Ty1/copia-like and Ty3/gypsy-like Retrotransposons in Tomato Plants, Kakutani K, Nakagawa M, Nanjo A, Sasao M, Takahata T, Matsuda Y, Nonomura T, Toyoda H, Annals of the Phytopathological Society of Japan, 68, 2,   2002 08 25 , http://ci.nii.ac.jp/naid/110002752862
  • (273)Expression Analysis of Retrotransposons in Tomato, Nanjo A, Kakutani K, Nakagawa M, Sasao M, Takahata T, Matsuda Y, Nonomura T, Toyoda H, Annals of the Phytopathological Society of Japan, 68, 2, 229, 230,   2002 08 25 , http://ci.nii.ac.jp/naid/110002752863
  • トマトレトロトランスポゾンの発現解析, 南条綾子, 角谷晃司, 中川真樹, 笹尾真理, 野々村照雄, 松田克礼, 豊田秀吉, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 20th, 73,   2002 07 29 , http://jglobal.jst.go.jp/public/200902131097534259
  • 単一細胞RT‐PCR法による標的細胞発現遺伝子の解析 単一細胞内RT‐PCRにおける指標遺伝子の検索, 藤田和久, 松田克礼, 鮫島武, 木多景子, 角谷晃司, 野々村照雄, 豊田秀吉, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 20th, 46,   2002 07 29 , http://jglobal.jst.go.jp/public/200902146836270858
  • ガン特異的タンパクCD98遺伝子を導入した形質転換体の作出, 角谷晃司, 森浦展行, 益子高, 野々村照雄, 松田克礼, 豊田秀吉, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 20th, 160,   2002 07 29 , http://jglobal.jst.go.jp/public/200902181815775623
  • Microinjection-based In situ PCR for Gene Cloning in Targeted Single Cells of Barley Coleoptile. (VII) Control of Gene Amplification by Different Primers and Annealing Conditions(Abstracts Presented at the Meeting of the Kansai Division), Hirai Y, Matsuda Y, Aiba K, Fujita K, Kakutani K, Nonomura T, Toyoda H, Annals of the Phytopathological Society of Japan, 68, 1,   2002 04 25 , http://ci.nii.ac.jp/naid/110002752526
  • Analysis of Optimal Conditions for Treatment of Leaf Primordia of Detached Tomato Meristems with Chemical Mutagens and Tissue Culture of Variegated Leaves Differentiated from Mutagen-Treated Leaf Primordia., 角谷晃司, 池田千亜紀, 野々村照雄, 松田克礼, 豊田秀吉, 生物環境調節, 40, 1, 99, 105,   2002 03 31 , 10.2525/ecb1963.40.99, http://jglobal.jst.go.jp/public/200902182179114596
  • キャピラリー電気泳動による糖タンパク質糖鎖の超微量分析 (2) DNAシークエンサーを利用する分析, 木下充弘, 清一雄, 下戸友里, 角谷晃司, 掛樋一晃, 日本薬学会年会要旨集, 122nd, 3, 78, 78,   2002 03 05 , http://jglobal.jst.go.jp/public/200902102665152684
  • キャピラリー電気泳動による糖タンパク質糖鎖の超微量分析 (1), 木下充弘, 清一雄, 下戸友里, 角谷晃司, 掛樋一晃, 日本薬学会年会要旨集, 122nd, 3, 78, 78,   2002 03 05 , http://jglobal.jst.go.jp/public/200902112536183277
  • アグロバクテリウム法を用いたシナカンゾウ(Glycyrrhiza echinata)の効率的な形質転換, 角谷晃司, 尾崎和男, 渡辺斉, 友田勝巳, 近畿大学薬学総合研究所紀要, 10, 47, 53,   2002 03 05 , http://jglobal.jst.go.jp/public/200902125387919324
  • Sphingobacterium multivolum KST‐009からのキトサナーゼ遺伝子のクローニングとその構造解析, 松田克礼, 飯田祐一郎, 篠木武, 瀧川義浩, 角谷晃司, 野々村照雄, 豊田秀吉, 日本農芸化学会大会講演要旨集, 2002, 132,   2002 03 05 , http://jglobal.jst.go.jp/public/200902137154446122
  • バクテリオファージを利用したキチナーゼ遺伝子導入Enterobacter cloacae KPM‐007E/chiの迅速検出, 瀧川義浩, 森裕文, 大津康成, 松田克礼, 野々村照雄, 角谷晃司, 土佐幸雄, 真山滋志, 豊田秀吉, 日本農芸化学会大会講演要旨集, 2002, 180,   2002 03 05 , http://jglobal.jst.go.jp/public/200902187344796445
  • Classification of Glycyrrhiza Plants by Random Amplified Polymorphic DNA Analysis. Discrimination of Glycyrrhiza glabra L. and G.uralensis Fischer., 角谷晃司, 尾崎和男, 渡辺斉, 芝野真樹雄, 草野源次郎, 友田勝巳, Nat Med, 55, 6, 287, 293,   2001 12 20 , http://jglobal.jst.go.jp/public/200902159286258221
  • キャピラリーDNAシーケンサーを用いる糖タンパク質糖鎖の超微量分析, 木下充弘, 清一雄, 下戸友里, 中野三弥子, 角谷晃司, 小田泰雄, 掛樋一晃, キャピラリー電気泳動シンポジウム講演要旨集, 21st, 95, 96,   2001 12 12 , http://jglobal.jst.go.jp/public/200902158635829103
  • キャピラリー電気泳動による糖タンパク質のグライコフォーム解析, 木下充弘, 清一雄, 下戸友里, 中野三弥子, 角谷晃司, 掛樋一晃, キャピラリー電気泳動シンポジウム講演要旨集, 21st, 63, 64,   2001 12 12 , http://jglobal.jst.go.jp/public/200902184017841985
  • カンゾウ属植物のRandom Amplified Polymorphic DNA(RAPD)分析による分類 特に,Glycyrrhiza glabra L.とG.uralensis Fischerの鑑別, 角谷 晃司, 尾崎 和男, 渡辺 斉, 芝野 真樹雄, 草野 源次郎, 友田 勝巳, Natural Medicines, 55, 6, 287, 293,   2001 12
    Summary:標題の識別目的に両者のRAPD分析を行った結果,各々の種に特異的な増幅DNA断片が得られ,この断片の塩基配列から合成したプライマーが識別プライマーとして利用できることが示唆された
  • カンゾウ培養細胞で発現している配糖化酵素遺伝子の解析(2), 黄 鵬宇, 林 宏明, 井上 謙一郎, 角谷 晃司, 友田 勝巳, 日本生薬学会年会講演要旨集, 48回, 66, 66,   2001 09
  • カンゾウ属植物のRAPD分析による分類 特に,Glycyrrhiza glabra L.種とG.uralensis F.種の識別, 角谷 晃司, 友田 勝巳, 尾崎 和男, 渡辺 斉, 日本生薬学会年会講演要旨集, 48回, 136, 136,   2001 09
  • カンゾウ属植物のRAPD分析による分類 特に,Glycyrrhiza glabra L.種とG.uralensis F.種の識別, 角谷晃司, 友田勝巳, 尾崎和男, 渡辺斉, 日本生薬学会年会講演要旨集, 48th, 136,   2001 08 07 , http://jglobal.jst.go.jp/public/200902163237690735
  • カンゾウ培養細胞で発現している配糖化酵素遺伝子の解析 (2), 黄鵬宇, 林宏明, 井上謙一郎, 角谷晃司, 友田勝巳, 日本生薬学会年会講演要旨集, 48th, 66,   2001 08 07 , http://jglobal.jst.go.jp/public/200902197425546210
  • オオムギうどんこ病抵抗性発現におけるトリプトファンデカルボキシラーゼ遺伝子の役割, 瀧川義浩, 松田克礼, 木多景子, 角谷晃司, 野々村照雄, 豊田秀吉, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 19th, 106,   2001 07 29 , http://jglobal.jst.go.jp/public/200902152637833860
  • マイクロインジェクション・RT‐PCR法による標的細胞発現遺伝子の解析 オオムギcDNAマクロアレイによるうどんこ病菌感染誘導性遺伝子の発現解析, 平井康晴, 松田克礼, 森浩一, 角谷晃司, 野々村照雄, 豊田秀吉, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 19th, 107,   2001 07 29 , http://jglobal.jst.go.jp/public/200902190840316055
  • カンゾウ(Glycyrrhiza glabra L.)根のグルクロン酸転移酵素の精製と諸性質, 角谷晃司, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 19th, 93,   2001 07 29 , http://jglobal.jst.go.jp/public/200902192630828820
  • Burkholderia cepaciaからクローニングしたキチナーゼ遺伝子の塩基配列決定, 松田克礼, 豊田秀吉, 野々村照雄, 角谷晃司, 池田成志, 玉井隆行, 桑原精宏, 大内成志, 近畿大学農学総合研究所報告, 9, 129, 140,   2001 03 15 , http://jglobal.jst.go.jp/public/200902173250548919
  • 甘草のグルクロン酸転移酵素の精製と性質, 角谷晃司, 渡辺斉, 友田勝巳, 日本農芸化学会誌, 75, 191,   2001 03 05 , http://jglobal.jst.go.jp/public/200902182807637560
  • DNA sequence of chitinase gene cloned from Burkholderia cepacia, 松田 克礼, 豊田 秀吉, 野々村 照雄, 角谷 晃司, 池田 成志, 玉井 隆行, 桑原 精宏, 大内 成志, Bulletin of the Institute for Comprehensive Agricultural Sciences, Kinki University, 9, 129, 140,   2001 03 , http://ci.nii.ac.jp/naid/120005736285
    Summary:[Synopsis] In this study, we cloned a chitinase gene from Burkholderia cepacia and identified a nucleotide sequence. A genomic library was constructed by the use of λEMBL3, and then phage clones producing chitinase were selected by using 4-MU chitotrioside as a substrate. The inserted DNA was subcloned into a pBluescript vector with restriction enzymes to make pBlueCAK12 (4.4 kb) clone including a chitinase gene. The DNA fragment was deleted by exonuclease III and the chitinase-coding region was determined by chitinase activity detected by the use of 4-MU chitotrioside. The nucleotide sequence of chitinase gene (2.98 kb) was determined by primer walking method, in which ORF of chitinase gene was amplified by specific primers including the expected start and stop codons. The amplified DNA was inserted into a pBluescript vector, and then chitinase activity was detected by the use of 4-MU chitotrioside.
  • カンゾウ属植物のRandom Amplified Polymorphic DNA(RAPD)分析による分類 特に,Glycyrrhiza glabra L.種とG.uralensis F.種の識別, 角谷晃司, 尾崎和男, 渡辺斉, 友田勝巳, 近畿大学薬学総合研究所紀要, 9, 95, 102,   2000 12 14 , http://jglobal.jst.go.jp/public/200902170215000111
  • Classification of Glycyrrhiza Plants by Randam Amplified PolymorphicDNA Analysis Discrimination of Glycyrrhiza glabra L. species and G.uralensis F. species, Kakutani Koji, Ozaki Kazuo, Watanabe Hitoshi, Tomoda Katsumi, Bulletin of Pharmaceutical Research and Technology Institute, 9, 95, 102,   2000 12 14 , http://ci.nii.ac.jp/naid/110000560722
  • 甘草主根の配糖化酵素遺伝子のクローニング, 角谷晃司, 友田勝巳, 渡辺斉, 日本生薬学会年会講演要旨集, 47th, 211,   2000 08 08 , http://jglobal.jst.go.jp/public/200902100832752378
  • カンゾウ培養細胞で発現している配糖化酵素遺伝子の解析, 林宏明, 黄鵬宇, 井上謙一郎, 角谷晃司, 友田勝巳, 日本生薬学会年会講演要旨集, 47th, 162,   2000 08 08 , http://jglobal.jst.go.jp/public/200902178986430682
  • ジスルフィラムの亜セレン酸惹起白内障ラット水晶体iNOS mRNA発現に対する影響, 伊藤吉将, 鍋倉智裕, 角谷晃司, 寺尾求馬, 友広雅之, 日本薬学会年会要旨集, 120th, 2, 185,   2000 03 05 , http://jglobal.jst.go.jp/public/200902116454399790
  • Somatic Embryogenesis and Plantlet Regeneration on Several Species of Licorice, Kakutani Koji, Ozaki Kazuo, Watanabe Hitoshi, Bulletin of Pharmaceutical Research and Technology Institute, 8, 73, 78,   1999 12 14 , http://ci.nii.ac.jp/naid/110000560707
  • RAPD法によるカンゾウ属植物の分類, 角谷晃司, 尾崎和男, 渡辺斉, 友田勝巳, 日本農芸化学会関西支部講演会講演要旨集, 411th, 94,   1999 10 01 , http://jglobal.jst.go.jp/public/200902101506116140
  • 甘草のグリチルリチン生合成酵素遺伝子の解析 (2), 角谷晃司, 友田勝巳, 渡辺斉, 日本生薬学会年会講演要旨集, 46th, 130,   1999 08 19 , http://jglobal.jst.go.jp/public/200902157445071127
  • スペインカンゾウ(Glycyrrhiza glabra L.)のグルクロン酸転移酵素遺伝子の解析, 角谷晃司, 渡辺斉, 友田勝巳, 日本農芸化学会関西支部講演会講演要旨集, 406th, 49,   1998 10 , http://jglobal.jst.go.jp/public/200902142051680996
  • Agrobacterium tumefaciensを用いたシナカンゾウへの外来遺伝子の導入, 角谷晃司, 友田勝巳, 渡辺斉, 日本生薬学会年会講演要旨集, 45th, 249,   1998 08 , http://jglobal.jst.go.jp/public/200902133886233010
  • 甘草(Glycyrrhiza glabra L.)の主根に存在するグルクロン酸転移酵素遺伝子の解析, 角谷晃司, 渡辺斉, 友田勝巳, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 16th, 55,   1998 07 , http://jglobal.jst.go.jp/public/200902125326664005
  • Preparation of licorice seedling by node culture, and Glycyrrhizin production by several nutricultures using the seedling, Koji Kakutani, Kazuo Ozaki, Hitoshi Watanabe, Katsumi Tomoda, Natural Medicines, 51, 447, 451,   1997 12 01 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031434390&origin=inward
    Summary:Seedlings of Glycyrrhiza glabra L. were efficiently propagated from the meristem tissue of buds by the node culture method, and transferred to nutriculture apparatus following acclimatization Attempts were made to produce glycyrrhizin in the tap-root of G. glabra L. by several nutricultures using the seedlings. In water culture, many adventitious roots were formed, but the tap-root did not swell. In spray culture, plant height did not extend and only thin roots were formed. However, in rockwool culture, the tap-root swelled well and its glycyrrhizin content reached 1.2% after 6-month-culture. This demonstrated that efficient production of glycyrrhizin, which is not possible by tissue culture or hairy root culture, is possible by a systematic culture of G. glabra L.
  • Preparation of Licorice Seedling by Node Culture, and Glycyrrhizin Production by Several Nutricultures Using the Seedling, KAKUTANI KOJI, OZAKI KAZUO, WATANABE HITOSHI, TOMODA KATSUMI, Natural Medicines, 51, 5, 447, 451,   1997 10 20 , http://ci.nii.ac.jp/naid/110008731779
    Summary:Seedlings of Glycyrrhiza glabra L. were efficiently propagated from the meristem tissue of buds by the node culture method, and transferred to nutriculture apparatus following acclimatization. Attempts were made to produce glycyrrhizin in the tap-root of G. glabra L. by several nutricultures using the seedlings. In water culture, many adventitious roots were formed, but the tap-root did not swell. In spray culture, plant height did not extend and only thin roots were formed. However, in rockwool culture, the tap-root swelled well and its glycyrrhizin content reached 1.2% after 6-month-culture. This demonstrated that efficient production of glycyrrhizin, which is not possible by tissue culture or hairy root culture, is possible by a systematic culture of G. glabra L.
  • Preparation of Licorice Seedling by Node Culture, and Glycyrrhizin Production by Several Nutricultures Using the Seedling., 角谷晃司, 尾崎和男, 渡辺斉, 友田勝巳, Nat Med, 51, 5, 447, 451,   1997 10 , http://jglobal.jst.go.jp/public/200902144078776610
  • PRTI 4th Exhibition The Present and the Past of Medicinal Plant, Kakutani Koji, Bulletin of Pharmaceutical Research and Technology Institute, 6,   1997 , http://ci.nii.ac.jp/naid/110000560687
  • Growth Characteristic and Glycyrrhizin Production of Licorice by Several Nutricultures., 角谷晃司, 尾崎和男, 渡辺斉, 友田勝巳, 近畿大学薬学総合研究所紀要, 5, 101, 104,   1996 12 , http://jglobal.jst.go.jp/public/200902128063346169
  • Glycyrrhizin production by the hydroponic culture using licorice culture seedling., 角谷晃司, 渡辺斉, 友田勝巳, 日本農芸化学会関西支部講演会講演要旨集, 396th, 21,   1996 10 , http://jglobal.jst.go.jp/public/200902132266291189
  • Growth and glycyrrhizin production of licorice according to various hydroponic culture methods., 角谷晃司, 友田勝巳, 尾崎和男, 渡辺斉, 日本生薬学会年会講演要旨集, 43rd, 79,   1996 08 , http://jglobal.jst.go.jp/public/200902114232352008
  • Somatic embryogenesis and regeneration on several species of licorice., 角谷晃司, 渡辺斉, 尾崎和男, 友田勝巳, 植物細胞分子生物シンポジウム講演要旨集, 5th, 119,   1996 07 , http://jglobal.jst.go.jp/public/200902112294466040
  • 10th PRTI Science Forum Medicinal Plants in Andes and Amazon, Tomoda K, Kakutani K, Bulletin of Pharmaceutical Research and Technology Institute, 5,   1996 , http://ci.nii.ac.jp/naid/110000560671
  • Growth Characteristic and Glycyrrhizin Production of Licorice by Several Nutricultures, Kakutani Koji, Ozaki Kazuo, Watanabe Hitoshi, Bulletin of Pharmaceutical Research and Technology Institute, 5, 101, 104,   1996 , http://ci.nii.ac.jp/naid/110000560668
  • Field Test for Bacterial Wilt-resistance of Selfed Progenies of Leaf Callus-derived Resistant Regenerants of Tomato, TOYODA H, DOHGO M, KATO Y, NOMURA T, MATSUDA Y, TANPO H, CHATANI K, KAKUTANI K, OUCHI S, Annals of the Phytopathological Society of Japan, 61, 6,   1995 12 25 , http://ci.nii.ac.jp/naid/110002731800
  • Examination of various conditions of the preparation of licorice culture seedlings utilizing tissue culture techniques., 角谷晃司, 友田勝巳, 尾崎和男, 渡辺斉, 日本生薬学会年会講演要旨集, 42nd, 115,   1995 08 , http://jglobal.jst.go.jp/public/200902118485010087
  • Biological purification of eutrophied water by melon and tobacco hairy roots., 豊田秀吉, 細井好之, 西村貴宗, 角谷晃司, 松田克礼, 東浦優, 大内成志, 植物組織培養学会大会,シンポジウム講演要旨集, 14th, 152,   1995 07 , http://jglobal.jst.go.jp/public/200902104463454428
  • Efficient production of licorice young plants using tissue culture., 角谷晃司, 尾崎和男, 渡辺斉, 友田勝巳, 植物組織培養学会大会,シンポジウム講演要旨集, 14th, 177,   1995 07 , http://jglobal.jst.go.jp/public/200902181919271210
  • Isolation of a Promoter Region from Chromosomal DNA of Pseudomonas solanacearum for Constructing an Effective Plasmid Vector, KAKUTANI Koji, TOYODA Hideyoshi, TAWA Naoko, KAWAKAMI Takao, MATSUDA Yoshinori, OUCHI Seiji, Annals of the Phytopathological Society of Japan, 61, 2, 137, 140,   1995 04 25 , 10.3186/jjphytopath.61.137, http://ci.nii.ac.jp/naid/110002731948
    Summary:トマト青枯病菌の染色体からプロモーターを分離するため, プロモーター欠失型 lux 遺伝子オペロンを有するトランスポゾン Tn4431 を接合伝達法で本菌染色体に導入した。発光能を獲得した形質転換体から染色体ライブラリーを作製し, 得られたクローンを lux 遣伝子とハイブリダイズさせ, 陽性クローンの DNA を GUS 遺伝子に連結して, その遺伝子発現活性の有無からプロモーターを選択した。その結果, 高いプロモーター活性の認められるサブクローンが得られたので, その全塩基配列を決定した。また, このプロモーターを既存のプラスミドに導入し, さらに若干の制限酵素部位を付加して, 青枯病菌で有効に機能できる形質転換ベクターを構築した。
  • Production of Disease Resistant Plants by Plant Cell Technology. I. Breeding of Disease Resistant Plants by the Use of Somaclonal Variation., 松田克礼, 豊田秀吉, 大内成志, 茶谷和行, 角谷晃司, 近畿大学農学総合研究所報告, 3, 59, 70,   1995 03 , http://jglobal.jst.go.jp/public/200902116212273767
  • 8th PRTI Science Forum Role of Active Oxygen and NO in Keeping of Life, Tomoda K, Kakutani K, Bulletin of Pharmaceutical Research and Technology Institute, 4,   1995 , http://ci.nii.ac.jp/naid/110000560657
  • PRTI 3th Exhibition, Kakutani K, Bulletin of Pharmaceutical Research and Technology Institute, 4, 100, 101,   1995 , http://ci.nii.ac.jp/naid/110000560659
  • Isolation of a Promoter Region from Chromosomal DNA of Pseudomonas solanacearum for Constructing an Effective Plasmid Vector., KAKUTANI Koji, TOYODA Hideyoshi, TAWA Naoko, KAWAKAMI Takao, MATSUDA Yoshinori, OUCHI Seiji, Japanese Journal of Phytopathology, 61, 2, 137, 140,   1995 , 10.3186/jjphytopath.61.137, http://ci.nii.ac.jp/naid/130003914290
    Summary:トマト青枯病菌の染色体からプロモーターを分離するため,プロモーター欠失型<i>lux</i>遺伝子オペロンを有するトランスポゾンTn4431を接合伝達法で本菌染色体に導入した。発光能を獲得した形質転換体から染色体ライブラリーを作製し,得られたクローンを<i>lux</i>C遺伝子とハイブリダイズさせ,陽性クローンのDNAをGUS遺伝子に連結して,その遺伝子発現活性の有無からプロモーターを選択した。その結果,高いプロモーター活性の認められるサブクローンが得られたので,その全塩基配列を決定した。また,このプロモーターを既存のプラスミドに導入し,さらに若干の制限酵素部位を付加して,青枯病菌で有効に機能できる形質転換ベクターを構築した。
  • Some Characteristics of Virulent Bacteriophages Isolated from Pseudomonas solanacearum-Infested Soil, KAKUTANI Koji, TOYODA Hideyoshi, GOTO Sachio, OUCHI Seiji, Annals of the Phytopathological Society of Japan, 60, 4, 531, 534,   1994 08 25 , 10.3186/jjphytopath.60.531, http://ci.nii.ac.jp/naid/110002737256
    Summary:Virulent bacteriophages (phages) were isolated from a field soil infested with the host bacterium, Pseudomonas solanacearum (strain K-101) and examined for their physical and chemical properties. The phages were classified into two types on the basis of their plaque morphologies (small obscure or large clear plaques against the indicator bacterium K-101), and the phages were isolated and purified through a series of plaque isolation. An electron microsopic observation indicated that the phage isolate forming a small plaque had a polygonal head with three tails and the other a smaller head with two tails. The electrophoretic patterns of DNA fragments obtained by the digestion with some restriction endonucleases were completely different between these two types of isolated phages. The RFLP was detected among the small plaque-forming phage isolates, although there was no difference in morphology of phage particle and infectivity against some different strains of the host bacterium.
  • Evaluation of Resistance in Selfed Progeny of Bacterial-Wilt Resistant Tomato Produced through Tissue Culture, TOYOTA H, KAWAKAMI T, DOGO M, KAKUTANI K, KATO Y, OUCHI S, Annals of the Phytopathological Society of Japan, 60, 3,   1994 06 25 , http://ci.nii.ac.jp/naid/110002737084
  • Antibacterial Activity of Indole and Its Related Compounds against Pseudomonas solanacearum (V) Identification of a Major Metabolite of Antibacterial 3-Indolepropionic Acid in Tomato Leaves, MATSUDA Kazuhiko, TOYODA Hideyoshi, NISHIDA Takatsugu, NISHIO Hitomi, DOGO Mitsue, KAKUTANI Koji, KOMAI Koichiro, OUCHI Seiji, Annals of the Phytopathological Society of Japan, 60, 2, 233, 235,   1994 04 25 , 10.3186/jjphytopath.60.233, http://ci.nii.ac.jp/naid/110002736904
    Summary:青枯病菌に対して選択的に抗細菌活性を示す3-インドールプロピオン酸(IPA)の代謝反応をトマト切枝を用いて調べた。薄層クロマトグラフィーを用いて分析したところ, トマト葉中にとり込まれたIPAは, 時間の経過とともにIPAよリRf値の低い化合物に変換されることがわかった。新たに検出された化合物はトマト葉中でIPAが代謝されたために生じたものと考え, IPAを24時間処埋したトマト葉から同代謝物を単離した後, 化学構造を解析した。その結果, IPAの主要代謝物は 1-O-(3-indolepropionyl)-β-D-glucopyranoseであると同定した。IPAは2.5μg/mlで青枯病菌の増殖を完全に抑制するのに対し, IPAの主要代謝物として同定されたグルコース配糖体は100μg/mlでも全く抗細菌活性を示さなかった。
  • Analysis of resistance mechanism in somaclonal variants of tomato against wilt-inducing bacterial pathogen, Pseudomonas solanacearum., 角谷晃司, 豊田秀吉, 加藤靖也, 道後充恵, 大内成志, 日本植物組織培養学会植物組織培養シンポジウム講演要旨集, 4th, 142, 143,   1994 , http://jglobal.jst.go.jp/public/200902131574066491
  • 4th PRTI Science Forum Erythropoietin-Form its Detection till Clinical Applications, Tomoda K, Kakutani K, Bulletin of Pharmaceutical Research and Technology Institute, 3,   1994 , http://ci.nii.ac.jp/naid/110000560641
  • PRTI 2th Exhibition, Tomoda K, Kakutani K, Bulletin of Pharmaceutical Research and Technology Institute, 3,   1994 , http://ci.nii.ac.jp/naid/110000560644
  • Antibacterial Activity of Indole and Its Related Compounds against Pseudomonas solanacearum. (V). Identification of a Major Metabolite of Antibacterial 3-Indolepropionic Acid in Tomato Leaves., MATSUDA Kazuhiko, TOYODA Hideyoshi, NISHIDA Takatsugu, NISHIO Hitomi, DOGO Mitsue, KAKUTANI Koji, KOMAI Koichiro, OUCHI Seiji, Japanese Journal of Phytopathology, 60, 2, 233, 235,   1994 , 10.3186/jjphytopath.60.233, http://ci.nii.ac.jp/naid/130003914216
    Summary:青枯病菌に対して選択的に抗細菌活性を示す3-インドールプロピオン酸(IPA)の代謝反応をトマト切枝を用いて調べた。薄層クロマトグラフィーを用いて分析したところ,トマト葉中にとり込まれたIPAは,時間の経過とともにIPAよりRf値の低い化合物に変換されることがわかった。新たに検出された化合物はトマト葉中でIPAが代謝されたために生じたものと考え,IPAを24時間処理したトマト葉から指代謝物を単離した後,化学構造を解析した。その結果,IPAの主要代謝物は1-O-(3-indolepropionyl)-&beta;-D-glucopyranoseであると指定した。IPAは2.5&mu;g/mlで青枯病菌の増殖を完全に抑制するのに対し,IPAの主要代謝物として指定されたグルコース配糖体は100&mu;g/mlでも全く抗細菌活性を示さなかった。
  • Efficient Production of Licorice Young Plants Using Tissue Culture : Composition of Culture Media, Kakutani Koji, Nagase Yoshinori, Watanabe Hitoshi, Bulletin of Pharmaceutical Research and Technology Institute, 3, 54, 57,   1994 , http://ci.nii.ac.jp/naid/110000560636
  • Evaluation of resistance in selfed progeny of bacterial-wilt resistant tomato produced through tissue culture., 角谷晃司, 豊田秀吉, 道後充恵, 茶谷和行, 田中徳夫, 加藤靖也, 大内成志, 植物組織培養学会大会,シンポジウム講演要旨集, 13th, 157,   1993 07 , http://jglobal.jst.go.jp/public/200902111156672287
  • Antibacterial Activity of Indole Derivatives agains Pseudomonas solanacearum, TOYODA H, DOHGO M, MATSUDA K, KAKUTANI K, NISHIDA T, OUCHI S, Annals of the Phytopathological Society of Japan, 59, 3,   1993 06 25 , http://ci.nii.ac.jp/naid/110002736520
  • Cloning of the Coat Protein Gene of a Virulent Phage of Pseudomonas solanacearum, KAKUTANI K, TOYODA H, NISHIDA T, OHCHI S, Annals of the Phytopathological Society of Japan, 59, 1,   1993 02 25 , http://ci.nii.ac.jp/naid/110002736220
  • 1th PRTI Science Forum Plant Catalyzer and Pharmacy, Tomoda K, Kakutani K, Bulletin of Pharmaceutical Research and Technology Institute, 2,   1993 , http://ci.nii.ac.jp/naid/110000560628
  • PRTI Exhibition, Tomoda K, Kakutani K, Bulletin of Pharmaceutical Research and Technology Institute, 2, 183, 184,   1993 , http://ci.nii.ac.jp/naid/110000560631
  • Binary Microbe System for Biological Control of Fusarium Wilt of Tomato: Enhanced Root-Colonization of an Antifungal Rhizoplane Bacterium Supported by a Chitin-degrading Bacterium., TOYODA Hideyoshi, MORIMOTO Masayuki, KAKUTANI Koji, MORIKAWA Masaaki, FUKAMIZO Tamo, GOTO Sachio, TERADA Hikojiro, OUCHI Seiji, Japanese Journal of Phytopathology, 59, 4, 375, 386,   1993 , 10.3186/jjphytopath.59.375, http://ci.nii.ac.jp/naid/130003749978
    Summary:An antagonistic rhizoplane bacterium (<i>Serratia Marcescens</i>) and a chitin-degrading microbe (<i>Streptomyces anulatus</i>) were used for establishing a binary microbe system for biological control of Fusarium wilt disease of tomato. For effectively tracing root-colonization, the rhizoplane bacterium was genetically marked with the <i>lux</i> genes and the tetracycline resistance gene through bacterial conjugation. Successful colonization by the bacterium on roots from bacterized seeds was visualized by bioluminescence of the transconjugant into which the <i>lux</i> gene operon had been integrated. Antifungal activity of the transconjugant was stable and effectire even when the bacterium was colonized on tomato roots. Multiplication of the transconjugant in soil was supported by the chitin-degrading microbe which had been previously added in chitin-supplemented soil. The chitin-degrading microbe produced extracellular endochitinases and hydrolyzed chitin which was added to soil. Chitohydrolytic products supported the multiplication and root-colonization of the rhizoplane bacterium in soil. The binary microbe system comprising of chitin-degrading microbe and gene-marked transconjugant was effective in controlling of tomato wilt disease caused by <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i>.
  • Introduction of Foreign Genes and Analysis of Their Expression, Toyoda Hideyoshi, Kakutani Koji, Matsuda Yoshinori, Bulletin of Pharmaceutical Research and Technology Institute, 2, 44, 60,   1993 , http://ci.nii.ac.jp/naid/110000560614
  • Antibacterial Activities of Indole and Its Related Compounds against Pseudomonas solanacearum (III) In Vivo Assay for Antibacterial Activity of Indole Derivatives Using Detached Tomato Leaves and Genetically Marked Pathogen, KAKUTANI Koji, TOYODA Hideyoshi, MATSUDA Kazuhiko, NISHIDA Takatsugu, DOGO Mitsue, SAKA Hideki, HAMADA Masayuki, OUCHI Seiji, Annals of the Phytopathological Society of Japan, 58, 5, 784, 788,   1992 12 25 , 10.3186/jjphytopath.58.784, http://ci.nii.ac.jp/naid/110002736060
    Summary:植物体に処理したインドール誘導体の抗細菌活性を検討するため, トマト切枝葉によるアッセイ系を確立した。青枯病菌の染色体に検出マーカー遣伝子(lux オペロンと tet^r 遺伝子)を接合伝達法で導入し, 病原菌の増殖をテトラサイクリン抵抗性と発光能によって追跡した。次に, 遺伝子標識細菌を切枝葉に接種して病原性が保持されていることを確認した後, 各種濃度のインドール, 3-インドールプロピオン酸および3-インドールアクリル酸を切枝葉に処理して病原菌増殖に対する影響を検討した。その結果, 3-インドールプロピオン酸処理によって病原菌の増殖ならびに萎凋出現がもっとも効果的に抑制された。これらの効果から, 本法は各種化合物の in vivo 抗細菌活性の測定に有効であると考えた。
  • Structure-antibacterial Activity Relationships of 3-Indolepropionic Acid Derivatives and Related Com pounds, MATSUDA K, NISHIDA T, TOYODA H, DOGO M, SAKA H, KAKUTANI K, HAMADA M, OUCHI S, Annals of the Phytopathological Society of Japan, 58, 4,   1992 10 25 , http://ci.nii.ac.jp/naid/110002735890
  • In vivo Assay for Inhibitory Effect of Indole Derivatives on Pseudomonas solanacearum, KAKUTANI K, TOYODA H, MATSUDA K, DOUGO M, NISHIDA T, SAKA H, HAMADA M, OUCHI S, Annals of the Phytopathological Society of Japan, 58, 4,   1992 10 25 , http://ci.nii.ac.jp/naid/110002735891
  • Isolation of Promoter Sequence from Chromosomal DNA of DNA of Pseudomonas solanacearum, KAKUTANI K, TOYODA H, TAWA N, OUCHI S, Annals of the Phytopathological Society of Japan, 58, 4,   1992 10 25 , http://ci.nii.ac.jp/naid/110002735899
  • Antibacterial Activities of Indole and Its Related Compounds against Pseudomonas solanacearum(III). In Vivo Assay for Antibacterial Activity of Indole Derivatives Using Detached Tomato Leaves and Genetically Marked Pathogen., KAKUTANI Koji, TOYODA Hideyoshi, MATSUDA Kazuhiko, NISHIDA Takatsugu, DOGO Mitsue, SAKA Hideki, HAMADA Masayuki, OUCHI Seiji, Japanese Journal of Phytopathology, 58, 5, 784, 788,   1992 , 10.3186/jjphytopath.58.784, http://ci.nii.ac.jp/naid/130003914090
    Summary:植物体に処理したインドール誘導体の抗細菌活性を検討するため,トマト切枝葉によるアッセイ系を確立した。青枯病菌の染色体に検出マーカー遺伝子(<i>lux</i>オペロンと<i>tet</i><sup>r</sup>遺伝子)を接合伝達法で導入し,病原菌の増殖をテトラサイクリン抵抗性と発光能によって追跡した。次に,遺伝子標識細菌を切枝葉に接種して病原性が保持されていることを確認した後,各種濃度のインドール,3-インドールプロピオン酸および3-インドールアクリル酸を切枝葉に処理して病原菌増殖に対する影響を検討した。その結果,3-インドールプロピオン酸処理によって病原菌の増殖ならびに萎凋出現がもっとも効果的に抑制された。これらの効果から,本法は各種化合物の<i>in vivo</i>抗細菌活性の測定に有効であると考えた。
  • Antibacterial Activities of Indole against Pseudomonas solanacearum (II). Inhibitory Effect of Indole Derivatives on Bacterial Growth, TOYODA Hideyoshi, MATSUDA Kazuhiko, DOGO Mitsue, KAKUTANI Koji, AKAZA Keiji, YAMASHITA Shuji, IMANISHI Yuka, MATSUDA Yoshinori, HAMADA Masayuki, OUCHI Seiji, Annals of the Phytopathological Society of Japan, 57, 5, 716, 719,   1991 12 25 , 10.3186/jjphytopath.57.716, http://ci.nii.ac.jp/naid/110002735344
    Summary:青枯れ病防除薬剤の開発を目的として, インドールおよびその誘導体10種について, P. solanacearumに対する抑制効果を検討した。その結果, インドール, 3-インドールプロピオン酸(I-3P)および3-インドールアクリル酸(I-3A)に強い抑制作用が認められたので, さらに7系統のP. solanacearumと4種のPseudomonas属細菌を供試し, これらの化合物の細菌増殖に及ぼす効果を調べた。その結果, I-3PとI-3Aはインドールの5〜10倍の抑制効果を示し, P. solanacearumの増殖のみを選択的に抑制することが明らかとなった。
  • Antibacterial Activities of Indole against Pseudomonas solanacearum(II). Inhibitory Effect of Indole Derivaties on Bacterial Growth., TOYODA Hideyoshi, MATSUDA Kazuhiko, DOGO Mitsue, KAKUTANI Koji, AKAZA Keiji, YAMASHITA Shuji, IMANISHI Yuka, MATSUDA Yoshinori, HAMADA Masayuki, OUCHI Seiji, Japanese Journal of Phytopathology, 57, 5, 716, 719,   1991 , 10.3186/jjphytopath.57.716, http://ci.nii.ac.jp/naid/130003914008
    Summary:青枯れ病防除薬剤の開発を目的として,インドールおよびその誘導体10種ついて,<i>P. solanacearum</i>に対する抑制効果を検討した。その結果,インドール,3-インドールプロピオン酸(I-3P)および3-インドールアクリル酸(I-3A)に強い抑制作用が認められたので,さらに7系統の<i>P. solanacearum</i>と4種の<i>Pseudomonas</i>属細菌を供試し,これらの化合物の細菌増殖に及ぼす効果を調べた。その結果,I-3PとI-3Aはインドールの5&sim;10倍の抑制効果を示し,<i>P. solanacearum</i>の増殖のみを選択的に抑制することが明らかとなった。
  • Stable phylloplane colonization by entomopathogenic bacterium Pseudomonas fluorescens KPM-018P and biological control of phytophagous ladybird beetles Epilachna vigintioctopunctata (Coleoptera : Coccinellidae), Y Otsu, Y Matsuda, H Mori, H Ueki, T Nakajima, K Fujiwara, M Matsumoto, N Azuma, K Kakutani, T Nonomura, Y Sakuratani, T Shinogi, Y Tosa, S Mayama, H Toyoda, BIOCONTROL SCIENCE AND TECHNOLOGY, 14, 5, 427, 439,   2004 08 , 10.1080/09583150410001683538, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=3042569347&origin=inward
    Summary:An entomopathogenic bacterium was isolated from tomato leaves and used as a microbial agent to control larvae of phytophagous ladybird beetles Epilachna vigintioctopunctata. The isolate was identified as Pseudomonas fluorescens KPM-018P on the basis of its bacteriological characteristics. KPM-018P produced extracellular chitinase to form a transparent zone around their colonies by hydrolyzing chitin in a minimal medium. Pale-yellow colonies turned red after a change of incubation temperature. These characteristics were availed as markers for tracking KPM-018P. The bacteria produced biosurfactants that enabled the bacteria to stably colonize the hydrophobic leaf surface; they were recovered without any considerable decrease even after a suspension of KPM-018P was sprayed onto leaves. KPM-018P, transformed with the gfp gene and observed with fluorescence microscopy, stably dwelled in the junctions of epidermal cells of bacteria-sprayed leaves. Ingestion of KPM-018P-sprayed leaves by the larvae caused prompt death of these insects to eventually suppress their pupation. This method is thus effective for decreasing the population of larvae and adult insect pests in the subsequent generation. The study provides an experimental basis for the biocontrol of herbivorous insect pests using a leaf-inhabiting, entomopathogenic strain of P. fluorescens.
  • Somatic embryogenesis and plantlet regeneration on several species of licorice, Koji Kakutani, Kazuo Ozaki, Hitoshi Watanabe, Katsumi Tomoda, Plant Biotechnology, 16, 3, 239, 241,   1999 , 10.5511/plantbiotechnology.16.239, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=53149124066&origin=inward
    Summary:Leaf explants of four species of licorice (Glycyrrhiza glabra L., G. uralensis F., G. echinata L., and G. squamulosa F.) were cultured on Murashige and Skoog (MS) basal medium. G. glabra L. and G. uralensis F. formed adventitious roots from calli on the medium containing NAA (1mgl-1). Both calli induced from G. echinata L. and G. squamulosa F. formed somatic embryos on the medium containing NAA (1mgl-1) and BAP (1mgl-1), or 2,4- D (0.5mgl-1) alone. Plantlets regenerated from the somatic embryos were transplanted to soil.
  • Evaluation of stable resistance expression in self-pollinated progenies of bacterial wilt resistant regenerants obtained from leaf callus of tomato, Hideyoshi Toyoda, Nobuhiro Kita, Koji Kakutani, Yoshinori Matsuda, Mitsue Dogo, Yasunari Kato, Tsuyoshi Nomura, Miki Bingo, Hiroyuki Tampo, Kazuyuki Chatani, Kunihiko Shimizu, Seiji Ouchi, Plant Biotechnology, 14, 2, 105, 110,   1997 , 10.5511/plantbiotechnology.14.105, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0011840488&origin=inward
    Summary:The bacterial wilt resistant line LNSR-7 of tomato was isolated from self-pollinated progenies of leaf-callus derived regenerants by directly inoculating a bacterial wilt pathogen Pseudomonas solanaceanum into injured roots of tested plants. The subsequent self-pollinated progenies of the line were examined for their fruit quality and resistance expression under natural cultivation conditions in a pathogen-infested tomato field. During three generations of progenies, the tomato plants showing both the bacterial wilt resistance and the high fruit qualities comparable to the parental cultivar were selected in order to fix commercial characteristics of the line. The stable inheritance of the resistance in the subsequent self-pollinated progenies was further examined by directly inoculating the pathogen into the roots of test plants. Inoculated plants were planted in soil heavily infested with the pathogen to ensure exposure to the pathogen. Under these artificial inoculation conditions, the selected line was shown to be highly resistant to the disease. The resistance mechanism in the line was analyzed by examining multiplication and translocation of the pathogen in planta. The precise monitoring of infection behavior of the pathogen was successfully achieved using the genetically marked P. solanacearum. Consequently the present line LNSR-7 strictly limited secondary multiplication and translocation of the pathogen and suppressed the wilt induction by the pathogen,.